Microbiota Emergencies in the Diagnosis of Lung Diseases: A Meta-Analysis

Abstract

Although many studies have reported that microbiota emergencies are deeply involved in the occurrence and subsequent progression of lung diseases, the present diagnosis of lung disease depends on microbiota markers, which is still poorly understood. Therefore, a meta-analysis was performed to confirm lung microbiota markers for the diagnosis of lung diseases. Literature databases were searched following the inclusion and exclusion criteria. There are 6 studies including 1347 patients and 26 comparisons to be enrolled, and then the diagnostic effect was evaluated using Stata 14.0 and Meta-disc 1.4 software. The pooled sensitivity (SEN), specificity (SPE), diagnostic likelihood ratio positive (DLR+), diagnostic likelihood ratio negative (DLR−), and diagnostic OR (DOR), as well as area under the curve (AUC) of microbiota markers in the diagnosis of lung diseases were 0.90 (95% CI: 0.83-0.94), 0.89 (95% CI: 0.76-0.95), 7.86 (95% CI: 3.39-18.21), 0.12 (95% CI: 0.06-0.21), 22.254 (95% CI: 12.83-39.59.14), and 0.95 (95% CI: 0.93-0.97), respectively. Subgroup analysis revealed that research based on Caucasian, adult, BAL fluid, PCR, pneumonia obtained higher AUC values. The microbiota markers have shown potential diagnosis value for lung diseases. But further large-scale clinical studies are still needed to verify and replicate the diagnostic value of lung microbiota markers.

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“… [ 82 ] [ 83 ] [ 84 ] microRNAs LAMP, LFA, RCA, DSN, EXPAR, SDA RT-qPCR, northern blot, NGS, microarrays Other techniques that have been used include single-molecule sequencing, ISH [ 85 ] [ 86 ] [ 87 ] Testosterone IFA ID-HPLC-LC.MS/MS, RIA, LC-MS/MS POC device reported is the ichroma II [ 57 ] [ 88 ] Pulmonary microbiota No commercial POC devices have been reported RT-PCR, ELISA, NGS The main detection strategy is the sequencing of the 16S rRNA gene. [ 89 ] [ 90 ] [ 91 ] ACE2 receptor No commercial POC devices have been reported ELISA RT-qPCR and IHC are used to evaluate the presence of ACE2 in tissues. [ 92 ] [ 93 ] [ 94 ] Vitamin D IFA, LFA RIA, competitive protein binding assay Other techniques have also been studied, such as LC-MS/MS, HPLC-UV, ELISA, CLIA.…”

Microbiota in the pathogenesis of COPD and its impact on the course of the disease

“…(Firmicutes), Aggregatibacter spp. (Proteobacteria), а также продукция медиаторов Th2 воспаления [67,68].…”