Microbial lipases: An overview of screening, production and purification

Bharathi, Devaraj; Rajalakshmi, G.

doi:10.1016/j.bcab.2019.101368

Cited by 126 publications

(81 citation statements)

References 88 publications

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“…To separate lipase, cells are removed by either filtration or centrifugation then the purification strategies are applied to concentrate the cell-free culture. About 80% of these strategies use the precipitation technique with 60% of this percent involves the ammonium sulfate method [2,3]. Specificity is a discriminative character of lipases and it can be categorized into positional, substrate, and stereo-specificity.…”

Section: Introductionmentioning

confidence: 99%

Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production

Helal

Abdelhady

Abou-Taleb

et al. 2021

Journal of Genetic Engineering and Biotechnology

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Background Rhizopus species is among the most well-known lipase producers, and its enzyme is suitable for use in many industrial applications. Our research focuses on the production of lipase utilizing waste besides evaluating its applications. Results An extracellular lipase was partially purified from the culture broth of Rhizopus oryzae R1 isolate to apparent homogeneity using ammonium sulfate precipitation followed by desalting via dialysis. The partially purified enzyme was non-specific lipase and the utmost activity was recorded at pH 6, 40 °C with high stability for 30 min. The constants Km and Vmax, calculated from the Lineweaver-Burk plot, are 0.3 mg/mL and 208.3 U/mL, respectively. Monovalent metal ions such as Na+ (1 and 5 mM) and K+ (5 mM) were promoters of the lipase to enhance its activity with 110, 105.5, and 106.5%, respectively. Chitosan was used as a perfect support for immobilization via both adsorption and cross-linking in which the latter method attained immobilization efficiency of 99.1% and reusability of 12 cycles. The partially purified enzyme proved its ability in forming methyl oleate (biodiesel) through the esterification of oleic acid and transesterification of olive oil. Conclusion The partially purified and immobilized lipase from Rhizopus oryzae R1 approved excellent efficiency, reusability, and a remarkable role in detergents and biodiesel production.

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Section: Introductionmentioning

confidence: 99%

Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production

Helal

Abdelhady

Abou-Taleb

et al. 2021

Journal of Genetic Engineering and Biotechnology

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“…Geralmente esse grupo de enzimas não requerem a presença de cofatores, atuam em ampla faixa de pH, sendo estáveis em altas temperaturas, possuem elevadas especificidade, características desejáveis que fazem com que sejam altamente aplicáveis em processos industriais (BHARATHI, RAJALAKSHMI, 2019;THAPA et al, 2019;PATEL et al, 2019;NIYONZIMA, VEENA, SUNIL, 2020).…”

Section: Introductionunclassified

Detecção De Esterase E Lipase Produzidas Por Fungos Filamentosos Isolados De Solos Da Caatinga / Detection of Esterase and Lipase Produced by Filamentous Fungi Isolated of Caatinga Soil

França

Marinho

Cândido

et al. 2020

BJD

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RESUMO As esterases (E.C. 3.1.1.1) são enzimas pertencentes à classe das carboxil ester hidrolases, e atuam diretamente na clivagem e na formação de ligações ésteres, enquanto as lipases (EC 3.1.1.3) são hidrolases que catalisam a hidrólise de triacilgliceróis em ácidos graxos livres e glicerol. A utilização de enzimas nas indústrias permite o desenvolvimento de processos tecnológicos tão eficientes quanto os realizados pela natureza e sem impactos ambientais. Nesse contexto, o objetivo deste estudo foi identificar micro-organismos isoaldos da Caatinga com potencial promissor na produção das enzimas esterase e lipase. Amostras de fungos filamentosos (6 amostras) do gênero Aspergillus sp. denominados de SIS (10, 11, 12, 14, 92, 112) e 2 isolados de Penicillium sp. denominados de SIS (21, 23) foram cultivados em meios de cultura específico utilizando as temperaturas de 28°C e 35°C, durante 72 h, com acompanhamento diário. A presença do halo característico evidenciou a presença das enzimas testadas. Os melhores resultados obtidos indicaram que a produção de lipase e esterase com o Penicillium sp. (SIS 23), apresentando índice enzimático de 2,0cm à 35°C e á 28°C e 1,5 cm após 72 horas de cultivo destacando-se como um potencial produtor de esterase e lipase. Palavras-chave: detecção de enzimas microbianas, fungos filamentosos, enzimas hidrolíticas ABCTRACT Esterases (E.C. 3.1.1.1) are enzymes belong to the class of carboxyl ester hydrolases and act directly in cleavage and formation of ester bonds, while the lipases (EC 3.1.1.3) are hydrolases that catalyze the hydrolysis of triacylglycerols to free fatty acids and glycerol). The use of enzymes in industries allows the development of technological processes as efficient as those carried out by nature and without environmental impacts. In this context, the objective this study was identify microorganisms isolated from Caatinga with promising potential in production of enzymes esterase and lipase. Samples of filamentous fungi (6 samples) Aspergillus sp. genus, called SIS (10, 11, 12, 14, 92, 112) and 2 isolates of Penicillium sp., called SIS (21, 23) were grown in specific culture means using temperatures of 28 °C and 35 °C during 72 h, with daily monitoring. The presence of the characteristic halo showed the presence of the tested enzymes. The best results obtained indicated the production of lipase and esterase by Penicillium sp. (SIS 23) with enzyme index of 2.0 cm at 35 °C and at 28 ° C and 1.5 cm after 72 hours cultivation standing out as potential producer of esterase and lipase.

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“…Lipidic sources are fundamental inducers in lipase production and can also be used as the only carbon source in cultivation of lipase-producing microorganisms [43][44][45][46]. Many different vegetable oils are reported to be added in lipase production by fermentation, and fatty acid-derived surfactants, like Tween80, are popular enzyme inducers [45][46][47][48]. The high availability and low price of soapstock made it an interesting lipid source which was investigated in the last decades as profitable nutrient in large-scale lipase production.…”

Section: Lipase Production From Soapstockmentioning

confidence: 99%

Enzymatic Methods for the Manipulation and Valorization of Soapstock from Vegetable Oil Refining Processes

Casali

Brenna

Parmeggiani

et al. 2021

Sustainable Chemistry

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The review will discuss the methods that have been optimized so far for the enzymatic hydrolysis of soapstock into enriched mixtures of free fatty acids, in order to offer a sustainable alternative to the procedure which is currently employed at the industrial level for converting soapstock into the by-product known as acid oil (or olein, i.e., free fatty acids removed from raw vegetable oil, dissolved in residual triglycerides). The further biocatalyzed manipulation of soapstock or of the corresponding acid oil for the production of biodiesel and fine chemicals (surfactants, plasticizers, and additives) will be described, with specific attention given to processes performed in continuous flow mode. The valorization of soapstock as carbon source in industrial lipase production will be also considered.

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Microbial lipases: An overview of screening, production and purification

Cited by 126 publications

References 88 publications

Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production

Lipase from Rhizopus oryzae R1: in-depth characterization, immobilization, and evaluation in biodiesel production

Detecção De Esterase E Lipase Produzidas Por Fungos Filamentosos Isolados De Solos Da Caatinga / Detection of Esterase and Lipase Produced by Filamentous Fungi Isolated of Caatinga Soil

Enzymatic Methods for the Manipulation and Valorization of Soapstock from Vegetable Oil Refining Processes

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