Microbial biofloc as source of probiotic bacteria for the culture of Litopenaeus vannamei

Ferreira, Gabriela Lícia Santos; Bolívar, Norha; Pereira, Scheila Anelise; Guertler, Cristhiane; Vieira, Felipe do Nascimento; Mouriño, José Luiz Pedreira; Seiffert, Walter Quadros

doi:10.1016/j.aquaculture.2015.06.006

Cited by 100 publications

(69 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Generally, the increase in C/N ratio by supplementing external carbon results in a reduction in toxic inorganic nitrogen in aquaculture systems (Crab, Defoirdt, Bossier, & Verstraete, 2012;Xu et al, 2016;Yu et al, 2016). In some studies, the existence of probiotics can promote fish growth or inhibit pathogens, depending on the proportion of probiotics in the system and C/N ratio (Ferreira et al, 2015;Yu et al, 2016). In the other studies, a high C/N ratio benefited the growth of some heterotrophic bacteria that could become opportunistic fish pathogens (Aguilera-Rivera et al, 2014;Murray & Peeler, 2005).…”

mentioning

confidence: 99%

The effect of C/N ratio on bacterial community and water quality in a mussel-fish integrated system

et al. 2018

View full text Add to dashboard Cite

A 30‐day experiment was conducted to evaluate the effect of C/N ratio on water quality and bacterial community in an integrated system comprising one molluscan species (pearl mussel Hyriopsis cumingii) and two fish species (gibel carp Carassius gibelio and silver carp Hypophthalmichthys molitrix) at five C/N ratios (6, 8, 10, 12 and 14). The mussel and fishes were reared in the experimental tanks (400 L), but gibel carp received formulated feed. Water quality in the experimental tanks was analysed on day 0, 10, 20 and 30, and bacterial community in the water column and sediment was analysed on day 30. Total nitrogen, total phosphorus and total organic carbon accumulated in the tanks over time. Ammonia and nitrite decreased with the increase in C/N ratio. Bacterial community in the water column and sediment changed at the phylum and genus levels with the increase in C/N ratio, and the critical C/N ratio causing a functional shift of bacterial community occurred at 10 in water column and 12 in sediment. The increase in C/N ratio benefited the growth of both potential probiotics and pathogenic bacteria. The high C/N ratio enhanced the bacterial functions of chemoheterotrophy and hydrocarbon degradation, but depressed the functions of nitrification and denitrification in the water column and sediment respectively. This study reveals that the C/N ratio can be used as a tool to manipulate the bacterial community and water quality in the mussel‐fish integrated system.

show abstract

mentioning

confidence: 99%

The effect of C/N ratio on bacterial community and water quality in a mussel-fish integrated system

et al. 2018

View full text Add to dashboard Cite

show abstract

“…However, most studies on probiotics and marine crustaceans have been performed on a small laboratory scale and under controlled laboratory conditions (Vieira et al, 2007(Vieira et al, , 2008(Vieira et al, , 2010Pham et al, 2014;Ferreira et al, 2015). Because laboratory conditions do not always match the conditions on commercial farms, there is a need for studies on the use of probiotics on commercial farms.…”

Section: Introductionmentioning

confidence: 99%

“…Gatesoupe (1999) defined a probiotic as "microbial cells that are administered in such a way as to be kept alive, with the aim of improving health". The use of probiotics produces benefits for marine shrimp by improving the balance of the intestinal microbiota (Vieira et al, 2008), survival (Pham et al, 2014), resistance to infection by pathogens (AguileraRivera et al, 2014), immune stimulation (Ferreira et al, 2015), and diet digestibility (Buglione Neto, 2009). Unlike antibiotics, this method does not leave any residues in the shrimp meat (Kesarcodi-Watson et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Use of probiotic-supplemented diet on a Pacific white shrimp farm

et al. 2016

Self Cite

View full text Add to dashboard Cite

-The objective of this study was to evaluate the use of a diet supplemented with Lactobacillus plantarum on performance, immunological parameters, and bacterial microbiota of the digestive tract of white leg shrimp (Litopenaeus vannamei). We used six earthen ponds (1.2 ha) stocked with 14 shrimp m −2 (3 g mean weight). Three ponds received a diet supplemented with L. plantarum and three were maintained as control. The survival and feed efficiency of shrimp fed the supplemented diet were greater than that observed in shrimp fed the control diet, with survival values of 83.02±6.12% and 74.65±9.07% and feed efficiencies of 117.97±4.45% and 104.46±7.30%, respectively. However, we observed no differences in weight gain. The intestines of shrimp fed probiotics had lower counts of Vibrio spp. and higher counts of lactic acid bacteria, compared with those of control shrimp. Diets supplemented with L. plantarum alter the intestinal bacterial microbiota of shrimp, resulting in increased survival and feed efficiency.

show abstract

“…The working mechanism in culturing P. similis using FWD is somewhat similar to Biofloc technique. Biofloc technique utilizes a micro-aggregate of organic material to enhance probiotic bacteria bloom (Aguilera-Rivera et al, 2014;Avnimelech, 2012;Ferreira et al, 2015) which are then used to confer growth benefits to the culture species. Fish waste has previously been used as a source of microbial organisms, amino acids and enzymes (Faid et al, 1997;Ghaly et al, 2013;Liao et al, 1997;Rebah & Miled, 2013).…”

Section: Discussionmentioning

confidence: 99%

Fish-processing wastes as an alternative diet for culturing the minute rotiferProales similisde Beauchamp

et al. 2018

View full text Add to dashboard Cite

We evaluated the suitability of fish waste diet (FWD) for culturing the minute rotifer Proales similis through the observation of their population growth and particle size selective feeding. A total of five treatments either with Nannochloropsis oculata or FWD (0.75 and 0.50 g/L) or the combination of N. oculata and FWD were set up in triplicates. P. similis were cultured in diluted natural seawater (8 g/L salinity) and 26 ± 1°C with the diet treatments being applied randomly. We daily monitored the rotifer density, the number of bacteria and the water quality in all the cultures. The population density of P. similis increased exponentially in all treatments, while the mean growth in FWD 0.75 g/L was significantly higher (p < .05) than that in the control. Addition of N. oculata to FWD resulted to lowered P. similis population growth. Bacterial colony count was high in FWD and introduction of P. similis to the diet decreased their density. The estimated bacteria ingestion rates were generally in the range of 6.03 × 102–1.24 × 104 bacteria/rotifer/hr and there was a positive linear relationship between bacterivory and rotifer population growth. We also observed a shift in the particle size distribution with a reduction in the frequency and concentration of small‐sized particles (<2.5 μm) at day 6. These results accentuate the potential of fish‐processing waste as diet for culturing P. similis which feeds on bacteria and small particles (≤2.5 μm) that are by‐products of degradation of this diet.

show abstract

Microbial biofloc as source of probiotic bacteria for the culture of Litopenaeus vannamei

Cited by 100 publications

References 36 publications

The effect of C/N ratio on bacterial community and water quality in a mussel-fish integrated system

The effect of C/N ratio on bacterial community and water quality in a mussel-fish integrated system

Use of probiotic-supplemented diet on a Pacific white shrimp farm

Fish-processing wastes as an alternative diet for culturing the minute rotiferProales similisde Beauchamp

Contact Info

Product

Resources

About