Microbial and enzymatic methods for the removal of caffeine

Gokulakrishnan, S.; Krishnan, Chandraraj; Gummadi, Sathyanarayana N.

doi:10.1016/j.enzmictec.2005.03.004

Cited by 105 publications

(64 citation statements)

References 72 publications

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“…For biodegradation of organic compounds, such as nonylphenol, structural isomers of the metabolites were expected to occur in wastewater samples as the treatments involve complicated degradation processes. 25 A similar situation was expected in the biodegradation of caffeine, 11 resulting in isomeric metabolites with a similar fragment in mass spectrometry, and separation of structural isomers by LC could be feasible for the detection of caffeine metabolites in wastewater. In environmental samples, the complexity of co-existing materials and trace level concentrations of target analytes were expected, so solid phase extraction (SPE) is important to extract target compounds from samples.…”

Section: Introductionmentioning

confidence: 99%

“…Effective removals of caffeine and theophylline in activated sludge were previously reported by other studies, which could be attributed to sorption and biodegradation in activated sludge. 51 Xanthine was formed through the biodegradation of methylxanthines, 11 which could contribute to the increase in xanthine after A2O treatment. In order to clarify the transformation trend of caffeine in activated sludge, the distribution of caffeine derivatives in water phase and solid phase (activated sludge) should be further evaluated.…”

Section: Application To Wastewater Samplesmentioning

confidence: 99%

“…Secondly, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine and xanthine are formed from theophylline, paraxanthine and theobromine by demethylases (N-1 demethylase, N-7 demethylase and N-3 demethylase, respectively). 11 Caffeine metabolites were well studied in previous studies on human metabolism, and a few studies reported several caffeine metabolites such as theophylline and paraxanthine in wastewater influents and effluents. [12][13][14][15][16][17] In addition, the occurrence of theobromine was reported in natural water.…”

Section: Introductionmentioning

confidence: 99%

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Determination of Caffeine and Its Metabolites in Wastewater Treatment Plants Using Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

Echigo

Itoh

2018

ANAL. SCI.

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For caffeine and its seven major metabolites (i.e., theobromine, theophylline, paraxanthine, 1-methylxanthine, 3-methylxanthine, 7-methylxanthine, and xanthine), an optimized analytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) for their detection in wastewater samples was developed in this study. Extraction of these compounds (recoveries ranged from 60.3 to 83.2%) was made possible by combining universal polymeric reversedphase cartridge and polymeric strong cation exchange cartridge. This method was applied to the determination of caffeine and its metabolites in the influent and effluent of an anaerobic-anoxic-oxic (A2O) process. In the A2O influent, caffeine and its metabolites (except xanthine) ranged from 1.39 to 5.45 μg/L, and their concentrations in the A2O effluent ranged from 10.2 to 171.3 ng/L. The mass load of caffeine was 14.9 g/day/1000 inhabitants, considering the population served by the target wastewater treatment plant (WWTP). The concentration of caffeine derivatives in wastewater influent is a tool for estimating the population size in the area served by WWTPs.

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Section: Introductionmentioning

confidence: 99%

Section: Application To Wastewater Samplesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Determination of Caffeine and Its Metabolites in Wastewater Treatment Plants Using Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

Echigo

Itoh

2018

ANAL. SCI.

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“…to overcome these problems (Gokulakrishnan et al, 2005). Microbial caffeine degradation using free cells remains, as yet, a difficult challenge and requires a lot of research.…”

Section: Introductionmentioning

confidence: 99%

Enhanced caffeine degradation by immobilised cells of <i>Leifsonia</i> sp. strain SIU

Ibrahim

Shukor

Syed

et al. 2016

J. Gen. Appl. Microbiol.

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“…In addition, these methods are not specific to caffeine, therefore other flavor and aroma compounds are removed, resulting in poor quality coffee and tea. Thus, microbiological and enzymatic methods of caffeine removal are highly preferred [11].…”

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confidence: 99%

How induced cells of Pseudomonas sp. increase the degradation of caffeine

Gummadi

Santhosh

2006

Open Life Sciences

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Abstract:Decaffeination is an important process for the removal of caffeine from wastes generated by coffee and tea industries. Microbial degradation of caffeine is more useful than conventional chemical treatment because of its low cost and because it does not involve the use of toxic solvents. However, biodegradation of caffeine remains a problem because of the difficulty of finding a strain that can resist high concentration of caffeine in addition to be able to degrade caffeine at higher rates. In this study, we used the induced cells of Pseudomonas sp. for the degradation of caffeine. The induced cells (8 mg/ml) showed complete degradation of a initial concentration of caffeine of 1.2 g/l in 6 hours. The optimum pH was 7.0, the agitation rate was 180 rpm and the optimum temperature for degradation was 35• C.Under these conditions and in the presence of magnesium, complete degradation of 1.2 g/l of caffeine was accomplished in 4 hours. Additional trials determined that induced cells completely degraded an initial concentration of caffeine of 10 g/l in 26 hours. This is the first report on a strain that can degrade high concentrations of caffeine (e.g., 10 g/l) at the maximum rate of 0.385 g/l per hour. These results suggest that the strain can be used to successfully in developing a biological process for the degradation of caffeine.

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Microbial and enzymatic methods for the removal of caffeine

Cited by 105 publications

References 72 publications

Determination of Caffeine and Its Metabolites in Wastewater Treatment Plants Using Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

Determination of Caffeine and Its Metabolites in Wastewater Treatment Plants Using Solid-Phase Extraction and Liquid Chromatography–Tandem Mass Spectrometry

Enhanced caffeine degradation by immobilised cells of <i>Leifsonia</i> sp. strain SIU

How induced cells of Pseudomonas sp. increase the degradation of caffeine

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