Enhanced caffeine degradation by immobilised cells of &lt;i&gt;Leifsonia&lt;/i&gt; sp. strain SIU

Ibrahim, Salihu; Shukor, Mohd Yunus; Syed, Mohd Arif; Johari, Wan Lutfi Wan; Shamaan, Nor Aripin; Sabullah, Mohd Khalizan; Ahmad, Siti Aqlima

doi:10.2323/jgam.62.18

Cited by 12 publications

(6 citation statements)

References 33 publications

(36 reference statements)

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“…Similar results were reported by Fareed et al [ 15 ], where immobilized cells of Enterobacter cloacae strain TA7 effectively degraded in N -methyl carbamates pesticides more rapidly than freely suspended cells counterpart. At 300 mg/L of phenol, the freely suspended cells showed a lower specific degradation rate, whereas, the immobilized cells demonstrated a higher phenol degradation rate [ 22 ]. Pseudomonas putida strain (CCRC14365) was able to degrade 600 mg/L of phenolic compounds in the free cells system and up to 1000 mg/L with immobilized cells [ 23 ].…”

Section: Resultsmentioning

confidence: 99%

“…The presence of heavy metals in polluted areas is one of the main limiting factors for bioremediation because several organisms might not tolerate high concentrations of metals; therefore, their ability to degrade pollutants might reduce due to the effect of metals [ 31 ], and consequently, the need to assess the effect of heavy metals on the biodegradation of organic compounds. Many researchers reported the effects of heavy metal on the microbial growth; yet, there is very limited information concerning their effects on the degradation of pesticides [ 22 , 32 ].…”

Section: Resultsmentioning

confidence: 99%

“…After two hours, the beads were recurrently rinsed with 0.1% ( v / v ) tween 80 solution to get rid of the oil phase from the microbeads. The beads were kept overnight in distilled water at 4 °C before being harvested by filtration and used for the degradation of carbofuran experiments [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

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Enhanced Carbofuran Degradation Using Immobilized and Free Cells of Enterobacter sp. Isolated from Soil

et al. 2020

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Extensive use of carbofuran insecticide harms the environment and human health. Carbofuran is an endocrine disruptor and has the highest acute toxicity to humans than all groups of carbamate pesticides used. Carbofuran is highly mobile in soil and soluble in water with a lengthy half-life (50 days). Therefore, it has the potential to contaminate groundwater and nearby water bodies after rainfall events. A bacterial strain BRC05 was isolated from agricultural soil characterized and presumptively identified as Enterobacter sp. The strain was immobilized using gellan gum as an entrapment material. The effect of different heavy metals and the ability of the immobilized cells to degrade carbofuran were compared with their free cell counterparts. The results showed a significant increase in the degradation of carbofuran by immobilized cells compared with freely suspended cells. Carbofuran was completely degraded within 9 h by immobilized cells at 50 mg/L, while it took 12 h for free cells to degrade carbofuran at the same concentration. Besides, the immobilized cells completely degraded carbofuran within 38 h at 100 mg/L. On the other hand, free cells degraded the compound in 68 h. The viability of the freely suspended cell and degradation efficiency was inhibited at a concentration greater than 100 mg/L. Whereas, the immobilized cells almost completely degraded carbofuran at 100 mg/L. At 250 mg/L concentration, the rate of degradation decreased significantly in free cells. The immobilized cells could also be reused for about nine cycles without losing their degradation activity. Hence, the gellan gum-immobilized cells of Enterobacter sp. could be potentially used in the bioremediation of carbofuran in contaminated soil.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Enhanced Carbofuran Degradation Using Immobilized and Free Cells of Enterobacter sp. Isolated from Soil

et al. 2020

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“…A promising immobilisation technique that has gained increasing attention recently is cell entrapment (Bibi et al 2015;Ibrahim et al 2016;Mohanty and Jena 2017;Karamba et al 2017). Many immobilised FDMs have been successfully used in the production of keratinase that is highly necessary in the biodegradation and biotransformation of heavy metal-free feather wastes (Prakash et al 2010;Shrinavas et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Effective production of keratinase by gellan gum-immobilised Alcaligenes sp. AQ05-001 using heavy metal-free and polluted feather wastes as substrates

et al. 2019

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The ability of gellan gum-immobilised cells of the heavy metal-tolerant bacterium Alcaligenes sp. AQ05-001 to utilise both heavy metal-free and heavy metal-polluted feathers (HMPFs) as substrates to produce keratinase enzyme was studied. Optimisation of the media pH, incubation temperature and immobilisation parameters (bead size, bead number, gellan gum concentration) was determined for the best possible production of keratinase using the one-factor-at-a-time technique. The results showed that the immobilised cells could tolerate a broader range of heavy metal concentrations and produced higher keratinase activity at a gellan gum concentration of 0.8% (w/v), a bead size of 3 mm, bead number of 250, pH of 8 and temperature of 30 °C. The entrapped bacterium was used repeatedly for ten cycles to produce keratinase using feathers polluted with 25 ppm of Co, Cu and Ag as substrates without the need for desorption. However, its inability to tolerate/utilise feathers polluted with Hg, Pb, and Zn above 5 ppm, and Ag and Cd above 10 ppm resulted in a considerable decrease in keratinase production. Furthermore, the immobilised cells could retain approximately 95% of their keratinase production capacity when 5 ppm of Co, Cu, and Ag, and 10 ppm of As and Cd were used to pollute feathers. When the feathers containing a mixture of Ag, Co, and Cu at 25 ppm each and Hg, Ni, Pb, and Zn at 5 ppm each were used as substrates, the immobilised cells maintained their operational stability and biological activity (keratinase production) at the end of 3rd and 4th cycles, respectively. The study indicates that HMPF can be effectively utilised as a substrate by the immobilised-cell system of Alcaligenes sp. AQ05-001 for the semi-continuous production of keratinase enzyme.

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“…Another process involves decaffeination through microbial fermentation or digestion with cell-free enzymes. This bio-decaffeination process might be a promising approach because of its high specificity, ecofriendliness, and cost-effectiveness [12,13].…”

Section: Introductionmentioning

confidence: 99%

Different Catabolism Pathways Triggered by Various Methylxanthines in Caffeine-Tolerant Bacterium Pseudomonas putida CT25 Isolated from Tea Garden Soil

et al. 2018

Journal of Microbiology and Biotechnology

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The degradation efficiency and catabolism pathways of the different methylxanthines (MXs) in isolated caffeine-tolerant strain Pseudomonas putida CT25 were comprehensively studied. The results showed that the degradation efficiency of various MXs varied with the number and position of the methyl groups on the molecule (i.e., xanthine > 7-methylxanthine ≈ theobromine > caffeine > theophylline > 1-methylxanthine). Multiple MX catabolism pathways coexisted in strain CT25, and a different pathway would be triggered by various MXs. Demethylation dominated in the degradation of N-7-methylated MXs (such as 7methylxanthine, theobromine, and caffeine), where C-8 oxidation was the major pathway in the catabolism of 1-methylxanthine, whereas demethylation and C-8 oxidation are likely both involved in the degradation of theophylline. Enzymes responsible for MX degradation were located inside the cell. Both cell culture and cell-free enzyme assays revealed that N-1 demethylation might be a rate-limiting step for the catabolism of the MXs. Surprisingly, accumulation of uric acid was observed in a cell-free reaction system, which might be attributed to the lack of activity of uricase, a cytochrome c-coupled membrane integral enzyme.

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Enhanced caffeine degradation by immobilised cells of <i>Leifsonia</i> sp. strain SIU

Cited by 12 publications

References 33 publications

Enhanced Carbofuran Degradation Using Immobilized and Free Cells of Enterobacter sp. Isolated from Soil

Enhanced Carbofuran Degradation Using Immobilized and Free Cells of Enterobacter sp. Isolated from Soil

Effective production of keratinase by gellan gum-immobilised Alcaligenes sp. AQ05-001 using heavy metal-free and polluted feather wastes as substrates

Different Catabolism Pathways Triggered by Various Methylxanthines in Caffeine-Tolerant Bacterium Pseudomonas putida CT25 Isolated from Tea Garden Soil

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