Microbacterium wangchenii sp. nov., isolated from faeces of Tibetan gazelles (Procapra picticaudata) on the Qinghai-Tibet Plateau

Abstract

Two strains of Gram-stain-positive, aerobic, non-spore-forming, non-motile, rod-shaped bacteria (designated dk512T and dk508) were isolated from the faeces of Tibetan gazelle (Procapra picticaudata) collected from the Qinghai-Tibet Plateau, PR China. The 16S rRNA gene sequences of the strains showed the highest identity to Microbacterium saccharophilum K-1T (98.0 and 97.9 % similarity, respectively). The phylogenetic analysis based on 16S rRNA gene sequences revealed that dk512T and dk508 were members of the genus Microbacterium , and most closely related to strains Microbacterium mitrae M4-8T and Microbacterium hatanonis FCC-01T. The strains grew optimally on brain-heart infusion (BHI) agar with 5.0 % (v/v) sheep blood at 30 °C, pH 7.0 and with 1.0 % (w/v) NaCl. The genome of type strain dk512T was 3.8 Mb with a G+C content of 70.6 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain dk512T and previously characterized Microbacterium species were <95 and <70 %, respectively. In strain dk512T, the detected primary cellular fatty acids were anteiso-C15 : 0 and anteiso-C17 : 0, the main respiratory quinones were MK-9 (37.9 %) and MK-10 (35.7 %), and the polar lipids included diphosphatidylglycerol, phosphatidylglycerol and three unidentified glycolipids. The major cell-wall sugars were rhamnose, ribose and galactose. Alanine, glutamic acid, glycine and ornithine were in the cell-wall peptidoglycan. Based on phenotypic data and phylogenetic inference, these two strains represent a novel species of the genus Microbacterium , named here as Microbacterium wangchenii sp. nov, where dk512T is designated the type strain (=CGMCC 1.16590T=JCM 33494T=KCTC 49313T).

Microbacterium tenebrionis sp. nov. and Microbacterium allomyrinae sp. nov., isolated from larvae of Tenebrio molitor L. and Allomyrina dichotoma, respectively

“…Members of the genus are widely distributed in diverse environments such as soil, sediment or activated sludge, clinical specimens, fresh water, and seawater [2]. Some species have been isolated from animal faeces [4, 5] and plants [6, 7]. A few species have been recovered from the insects such as a moss Eacles imperials [8, 9], dead Saperda caracharias [10, 11] and Protaetia brevitarsis seulensis [12].…”

Gordonia jinghuaiqii sp. nov. and Gordonia zhaorongruii sp. nov., isolated from Tibetan Plateau wildlife

“…While studying the microflora diversity of Tibetan Plateau wildlife (PR China; e.g. wild ass, antelope, gazelle, vultures, plateau pika, marmot and snow finch), quite a few novel species have been uncovered in our laboratory, including Nocardioides yefusunii , Streptococcus pantholopis , Microbacterium wangchenii , Actinomyces liubingyangii , Luteimonas chenhongjianii , Lactobacillus xujianguonis and Vagococcus xieshaowenii [11–17]. The 16S rRNA gene sequences (described below) of these four hitherto unknown micro-organisms (zg-686 T , zg-691, HY186 T and HY189) isolated from marmot ( Marmota himalayana ) and wild ass ( Equus kiang ) have low similarity to those of known species in the GenBank database.…”

Identification of Nocardioides marmotae sp. nov. and Nocardioides faecalis sp. nov., two new members of the genus Nocardioides

“…Culturomics has become an essential strategy for investigating microbial diversity, which has been employed previously by our research group in the isolation and characterization of several novel microbial species, including Cellulomonas shaoxiangyii , Luteimonas chenhongjianii , Microbacterium wangchenii , Nocardioides dongxiaopingii and Vagococcus xieshaowenii [22–26]. During a study exploring the Nocardioides microbial diversity on the Qinghai–Tibet Plateau that included wildlife ( Procapra picticaudata , Equus kiang , Pantholops hodgsonii , Ochotona curzoniae , etc.…”