Gordonia jinghuaiqii sp. nov. and Gordonia zhaorongruii sp. nov., isolated from Tibetan Plateau wildlife

Abstract

Four mesophilic and Gram-stain-positive strains (zg-686T/zg-691 and HY186T/HY189) isolated from Tibetan Plateau wildlife (PR China) belong to the genus Gordonia according to 16S rRNA gene and genomic sequence-based phylogenetic/genomic results. They have a DNA G+C content range of 67.4–68.3 mol% and low DNA relatedness (19.2–27.6 %) with all available genomes in the genus Gordonia . Strains zg-686T/zg-691 and HY186T/HY189 had C18 : 1ω9c, C18 : 0 10-methyl, C16 : 1 ω7c/C16 : 1ω6c and C16 : 0 as major cellular fatty acids. The polar lipids detected in strains zg-686T and HY186T included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidyl inositol mannoside and phosphatidylinositol. The respiratory quinones comprised MK8(H2) (10.8 %) and MK9(H2) (89.2 %) for strain zg-686T, and MK6 (7.7 %), MK8(H2) (8.4 %), MK8(H4) (3.1 %) and MK9(H2) (80.8 %) for strain HY186T. Optimal growth conditions were pH 7.0, 35–37 °C and 0.5–1.5 % NaCl (w/v) for strains pair zg-686T/zg-691, and pH 7.0, 28 °C and 1.5 % (w/v) NaCl for strains pair HY186T/HY189. Based on these genotypic and phenotypic results, these four strains could be classified as two different novel species in the genus Gordonia , for which the names Gordonia jinghuaiqii sp. nov. and Gordonia zhaorongruii sp. nov. are proposed. The type strains are zg-686T (=GDMCC 1.1715T =JCM 33890T) and HY186T (=CGMCC 4.7607T =JCM 33466T), respectively.

Gordonia pseudamarae sp. nov., a home for novel actinobacteria isolated from stable foams on activated sludge wastewater treatment plants

“…S3). In general, the chemotaxonomic properties of this isolate were in good agreement with those of Gordonia strains [1,14,54], including those classified previously as N. amarae [7,8]. To this end, isolate CON9 T produced meso-A 2 pm as the diamino acid of the peptidoglycan, arabinose, galactose and ribose as whole-organism sugars, and a polar lipid pattern composed of diphosphatidylglycerol, phosphatidylethanolamine, phoshphatidylinositol and phosphatidylinositol mannosides (Fig.…”

“…S3). In general, the chemotaxonomic properties of this isolate were in good agreement with those of Gordonia strains [1, 14, 54], including those classified previously as N. amarae [7, 8].…”

“… Gordonia is the type genus of the family Gordoniaceae [10, 11], which also includes the genera Jongsikchunia [11] and Williamsia [12]. Many of the 47 Gordonia species with validly published names (https://lpsn.dsmz.de/genus/gordonia) [13] have been defined using chemotaxonomic, morphological and phenotypic features [1, 14]. Gordonia species belong to a well-supported 16S rRNA gene clade that can be distinguished from lineages of other mycolic acid containing genera classified in the order Mycobacteriales [11, 15, 16], results underpinned by whole genome sequence data, as expressed in phylogenomic trees [11].…”

“…There is also evidence that they have the potential to produce bioactive and antimicrobial agents [20]. Gordonia species are widely distributed in natural habitats [19,21,22] and are associated with diverse invertebrates and vertebrates from marine and terrestrial sources [14,19]. They have also been implicated as opportunistic pathogens of humans [1,23,24].…”

Gordonia zhenghanii sp. nov. and Gordonia liuliyuniae sp. nov., isolated from bat faeces

“…The members are Gram-stain-positive, non-sporulating, non-motile, aerobic coccus or rod-shaped bacteria, with MK-9(H 2 ) or MK-9(H 4 ) as the major menaquinone, meso-diaminopimelic acid (meso-DAP) as the diamino acid of the peptidoglycan, and fatty acid patterns consisting of nonbranched saturated acids or additional tuberculostearic acid. Among the various sources of origin, several members of this taxon were isolated from clinical specimens [2][3][4][5] and might be considered as opportunistic pathogens, but most were isolated from the environment, including soil [6,7], faeces [8], water [9], sediment [10] and sludge [11]. Some environmental isolates were capable of producing secondary metabolites [12], such as natural rubber [13], benzothiophene [14] and pigment production [15].…”