Method for Activity Staining of Peroxidase and β-1,3-Glucanase Isozymes Polyacrylamide Electrophoresis Gels

Shimoni, Moshe

doi:10.1006/abio.1994.1295

Cited by 18 publications

(10 citation statements)

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“…Peroxidase activity was carried out by soaking the gel in 25 mL citrate-phosphate buffer, pH 5.4, containing 3% H 2 O 2 and 8 mL guaiacol. After 2 min, brown bands indicated peroxidase activity (Shimoni, 1994).…”

Section: Detection Of Peroxidase Activity On Polyacrylamide Gelmentioning

confidence: 99%

Peroxidase is involved in Pepper yellow mosaic virus resistance in Capsicum baccatum var. pendulum

Gonçalves

Rodrigues²,

Diz³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Pathogenesis-related proteins (PRs) are among the defense mechanisms of plants that work as an important barrier to the development of pathogens. These proteins are classified into 17 families according to their amino acid sequences, serology, and/or biological or enzyme activity. The present study aimed to identify PRs associated with the pathosystem of Capsicum baccatum var. pendulum: Pepper yellow mosaic virus (PepYMV). Forty-five-day-old plants from accession UENF 1624, previously identified as resistant to PepYMV, were inoculated with the virus. Control and infected leaves were collected for analysis after 24, 48, 72, and 96 h. The inoculated and control plants were grown in cages covered with anti-aphid screens. Proteins were extracted from leaf tissue and the presence of β-1,3-glucanase, chitinase, peroxidase, and lipid transport protein was verified.

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Section: Detection Of Peroxidase Activity On Polyacrylamide Gelmentioning

confidence: 99%

Peroxidase is involved in Pepper yellow mosaic virus resistance in Capsicum baccatum var. pendulum

Gonçalves

Rodrigues²,

Diz³

et al. 2013

Genet. Mol. Res.

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“…Staining of POD isoforms on the gels was conducted according to Shimoni (1994). Negative stain of SOD and CAT isoforms was performed as described by Beauchamp and Fridovich (1971), and Woodbury et al (1971), respectively.…”

Section: Polyacrylamide Gel Electrophoresis (Page)mentioning

confidence: 99%

Antioxidant and anatomical responses in shoot culture of the apple rootstock MM 106 treated with NaCl, KCl, mannitol or sorbitol

Molassiotis¹,

Sotiropoulos

Tanou³

et al. 2006

Biol. plant.

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To determine whether the major influence of high salinity is caused by the osmotic component or by salinity-induced specific ion toxicity, we compared the effects of mannitol, sorbitol, NaCl and KCl (all in concentratuions corresponded to osmotic potential -1.0 MPa) on the antioxidant and anatomical responses of the apple rootstock MM 106 explants grown in the Murashige and Skoog (MS) medium. All the compounds had a significant influence on explant's mineral composition and reduced the leaf water content, whereas mannitol and salts decreased chlorophyll (Chl) content and increased proline content. Superoxide dismutase (SOD), peroxidase (POD) and non-enzymatic antioxidant activities as well as H 2 O 2 content were increased in the leaves and stems. In addition, in the leaves of explants exposed to NaCl an additional Mn-SOD isoform was revealed, while specific POD isoforms were detected in the leaves and stems treated with NaCl or KCl. However, catalase activity was depressed in the salt-treated leaves. At the ultrastructural level, the NaCl-treated leaves had the thickest lamina, due to an extensive increase of the size of epidermal and mesophyll cells. Also, an increase of the relative volume of the intercellular spaces in response to NaCl was observed. The results suggest that Na accumulation is the first candidate for the distinct antioxidant and anatomical responses between saline and osmotically generated stress in the MM 106 explants.

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“…Mn-SOD activity was resistant to both inhibitors. Staining of POD isoforms was conducted by incubating the gels in 50 mM sodium acetate buffer (pH 5.0), with 3-amino-9-ethyl-carbazole in the presence of 10 mM H 2 O 2 (Shimoni 1994). CAT activity in native PAGE gels was determined in a reaction mixture containing 2% (w/v) potassium ferricyanide and 2% (w/v) ferric chloride (Woodbury et al 1971).…”

Section: Page Analysis Of Antioxidant Enzymesmentioning

confidence: 99%

Oxidative stress, antioxidant activity and Fe(III)-chelate reductase activity of five Prunus rootstocks explants in response to Fe deficiency

et al. 2005

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Method for Activity Staining of Peroxidase and β-1,3-Glucanase Isozymes Polyacrylamide Electrophoresis Gels

Cited by 18 publications

References 0 publications

Peroxidase is involved in Pepper yellow mosaic virus resistance in Capsicum baccatum var. pendulum

Peroxidase is involved in Pepper yellow mosaic virus resistance in Capsicum baccatum var. pendulum

Antioxidant and anatomical responses in shoot culture of the apple rootstock MM 106 treated with NaCl, KCl, mannitol or sorbitol

Oxidative stress, antioxidant activity and Fe(III)-chelate reductase activity of five Prunus rootstocks explants in response to Fe deficiency

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