Peroxidase is involved in Pepper yellow mosaic virus resistance in Capsicum baccatum var. pendulum

Gonçalves, Leandro Simões Azeredo; Rodrigues, Rosana; Diz, Mariângela S.S.; Robaina, Renata Rodrigues; Júnior, A. T. do Amaral; Carvalho, André O.; Gomes, Valdirene Moreira

doi:10.4238/2013.april.26.3

Cited by 19 publications

(12 citation statements)

References 41 publications

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“…Therefore, SDS-PAGE analysis showed similar protein patterns in PepYMV-infected and control plants. Similar results were reported by Gonçalves et al (2013), who evaluated the SDS-PAGE gel profile for chitinases and lipid transfer proteins in both control and PepYMVinoculated C. baccatum seedlings. These data showed similar protein profiles for the different inoculation times.…”

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confidence: 72%

“…Among the few studies related to biochemical defense mechanisms against PepYMV in peppers, Gonçalves et al (2013) reported an increase in peroxidase expression in C. baccatum when it is inoculated with the virus, indicating that pathogenesis-related (PR)-9 is involved in the defense mechanism for such plants. Proteinase inhibitors related to C. baccatum defense against PepYMV have not been studied.…”

Section: Introductionmentioning

confidence: 99%

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Trypsin inhibitors from Capsicum baccatum var. pendulum leaves involved in Pepper yellow mosaic virus resistance

et al. 2014

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ABSTRACT. Several plant organs contain proteinase inhibitors, which are produced during normal plant development or are induced upon pathogen attack to suppress the enzymatic activity of phytopathogenic microorganisms. In this study, we examined the presence of proteinase inhibitors, specifically trypsin inhibitors, in the leaf extract of Capsicum baccatum var. pendulum inoculated with PepYMV (Pepper yellow mosaic virus). Leaf extract from plants with the accession number UENF 1624, which is resistant to PepYMV, was collected at 7 different times (0, 24, 48, 72, 96, 120, and 144 h). Seedlings inoculated with PepYMV and control seedlings were grown in a growth chamber. Protein extract from leaf samples was partially purified by reversedphase chromatography using a C2/C18 column. Residual trypsin activity was assayed to detect inhibitors followed by Tricine-SDS-PAGE analysis to determine the N-terminal peptide sequence. Based on trypsin inhibitor assays, trypsin inhibitors are likely constitutively synthesized in C. baccatum var. pendulum leaf tissue. These inhibitors are likely a defense mechanism for the C. baccatum var. pendulumPepYMV pathosystem.

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confidence: 72%

Section: Introductionmentioning

confidence: 99%

Trypsin inhibitors from Capsicum baccatum var. pendulum leaves involved in Pepper yellow mosaic virus resistance

et al. 2014

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“…Genetic resource studies in pre-breeding and breeding have been conducted for C. baccatum, which were primarily aimed at morpho-agronomic characterization and disease resistance (Rêgo et al, 2009;Rodrigues et al, 2012;Bento et al, 2013;Gonçalves et al, 2013). Additionally, interspecific crossing for gene introgression of bacterial spot disease resistance from C. baccatum into C. annuum have been successfully obtained by Potnis et al (2012).…”

Section: Introductionmentioning

confidence: 99%

Genetic dissection of agronomic traits in Capsicum baccatum var. pendulum

et al. 2015

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ABSTRACT. Genetic mapping is very useful for dissecting complex agronomic traits. Genetic mapping allows for identification of quantitative trait loci (QTL), provide knowledge on a gene position and its adjacent region, and enable prediction of evolutionary mechanisms, in addition to contributing to synteny studies. The aim of this study was to predict genetic values associated with different agronomic traits evaluated in an F 2 population of Capsicum baccatum var. pendulum. Previously, a reference genetic map for C. baccatum was constructed, which included 183 markers (42 microsatellite, 85 inter-simple sequence repeat, and 56 random amplification of polymorphic DNA) arranged in 16 linkage groups. The map was used to identify QTL associated with 11 agronomic traits, including plant height, crown diameter, number of days to flowering, days to fruiting, number of fruits per plant, average fruit weight, fruit length, fruit diameter, fruit QTLs for agronomic traits in C. baccatum var. pendulum pulp thickness, soluble solids, and fruit dry weight. QTL mapping was performed by standard interval mapping. The number of small QTL effects ranged from 3-11, with a total of 61 QTL detected in 9 linkage groups. This is the first report involving QTL analysis for C. baccatum species.

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“…PODs play an important role in abiotic stress tolerance in various plant species, including rice (Oryza sativa) (Demiral and Turkan 2005), barley (Hordeum uhulgare) (Liang et al, 2003), tomato (Solanum lycopersicum) (Mittova et al, 2004), pepper (Capsicum baccatum) (Gonçalves et al, 2013), and Kentucky bluegrass (Poa pratensis) (Li et al, 2014). Some plant POD genes have been expressed using transgenic techniques.…”

Section: Introductionmentioning

confidence: 99%

Expression of an alfalfa (Medicago sativa L.) peroxidase gene in transgenic Arabidopsis thaliana enhances resistance to NaCl and H2O2

Teng¹,

Xiao²,

Guo³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Peroxidases (PODs) are enzymes that play important roles in catalyzing the reduction of H 2 O 2 and the oxidation of various substrates. They function in many different and important biological processes, such as defense mechanisms, immune responses, and pathogeny. The POD genes have been cloned and identified in many plants, but their function in alfalfa (Medicago sativa L.) is not known, to date. Based on the POD gene sequence (GenBank accession No. L36157.1), we cloned the POD gene in alfalfa, which was named MsPOD. MsPOD expression increased with increasing H 2 O 2 . The gene was expressed in all of the tissues, including the roots, stems, leaves, and flowers, particularly in stems and leaves under light/dark conditions. A subcellular analysis showed that MsPOD was localized outside the cells. Transgenic Arabidopsis with MsPOD exhibited increased resistance to H 2 O 2 and NaCl. Moreover, POD activity in the transgenic plants was significantly higher than that in wild-type Arabidopsis. These results show that MsPOD plays an important role in resistance to H 2 O 2 and NaCl.

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Peroxidase is involved in Pepper yellow mosaic virus resistance in Capsicum baccatum var. pendulum

Cited by 19 publications

References 41 publications

Trypsin inhibitors from Capsicum baccatum var. pendulum leaves involved in Pepper yellow mosaic virus resistance

Trypsin inhibitors from Capsicum baccatum var. pendulum leaves involved in Pepper yellow mosaic virus resistance

Genetic dissection of agronomic traits in Capsicum baccatum var. pendulum

Expression of an alfalfa (Medicago sativa L.) peroxidase gene in transgenic Arabidopsis thaliana enhances resistance to NaCl and H2O2

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