Expression of an alfalfa (Medicago sativa L.) peroxidase gene in transgenic Arabidopsis thaliana enhances resistance to NaCl and H2O2

Teng, Ke; Xiao, G Z; Guo, Weijun; Yuan, Jing-Zhe; Li, J; Chao, Yuehui; Han, Liebao

doi:10.4238/gmr.15028002

Cited by 10 publications

(2 citation statements)

References 27 publications

(33 reference statements)

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“…Overexpression of SOD gene increase SOD activity in rubber tree leaves, and activated all ROS scavenging enzymes (Leclercq et al 2012). Plant transgenic-POD was significantly higher tolerance to H2O2 than wild-type plant (Teng et al 2016). The high concentration and activity of antioxidant enzymes in the plant tissue were proved enhancing tolerance to stress such as over-exploitation.…”

Section: Resultsmentioning

confidence: 99%

Physiological status of high and low metabolism Hevea clones in the difference stage of tapping panel dryness

et al. 2018

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Tistama R, Mawaddah PAS, Ade-Fipriani L, Junaidi. 2019. Physiological status of high and low metabolism Hevea clones in the difference stage of tapping panel dryness. Biodiversitas 20: 267-273. Tapping panel dryness (TPD) caused productivity of rubber trees drop sharply. However, the increase of TPD stage has not been completely elucidated, especially in physiological aspects. TPD incident was higher occurred in high metabolism than low metabolism clones. The incident has been classified based on visual observation. This research aimed to explore the physiological characters to identifying the physiological changes of each TPD stage in the two metabolism types of rubber clones, IRR 42 (low metabolism) and IRR 118 (high metabolism). The physiological parameters such as thiol, Pi content and peroxidase activity were specifically in each clone and the tissue types (bark or laticifer). The physiological pattern of IRR 42 was a difference with that of IRR 118 for the increase of the TPD stages. Phosphate inorganic content in the latex and bark were a decline in the TPD affected trees, whereas the sucrose content was relatively constant. The pattern of changes of thiol content in IRR 42 was reverse with IRR 118, both in the latex or bark tissue. Peroxidases activity in the latex and bark negatively correlated with the TPD stage. The decline of Pi and peroxidase activity can be used to identify the TPD incident in rubber trees.

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Section: Resultsmentioning

confidence: 99%

Physiological status of high and low metabolism Hevea clones in the difference stage of tapping panel dryness

et al. 2018

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“…Many studies have confirmed that the overexpression of transcription factor (TF) genes (i.e., MxbHLH18 and MxWRKY53) [23,24], antioxidant enzyme genes (i.e., AhCuZnSOD and AtAPX3) [25,26], osmotic regulation genes (i.e., MdHXK1 and AtBAG6) [27,28] and ion transporter genes (i.e., OsNHX7 and CNGC1) [29,30] changed salinity tolerance in transgenic plants. In alfalfa, several genes that participate in the salinity stress response, such as MsCBL4 [31], MsPP2CA1 [32] and MsPOD [33] have also been reported.…”

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confidence: 99%

Comparative physiology and transcriptome analysis to identify the important coding and non-coding RNAs imparting tolerance to salinity stress in alfalfa (Medicago sativa L.)

Rong

et al. 2023

Preprint

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Background: Salinity is among the most harmful agents that impacts alfalfa’s productivity. However, little is known regarding how salinity tolerance trait is regulated by lncRNAs in alfalfa. To evaluate the mRNAs and lncRNAs associated with tolerance to salinity stress in alfalfa, the root transcriptomics of two alfalfa cultivars differing in salinity tolerance, i.e., GN5 (tolerant to salinity) and GN3 (sensitive to salinity), were investigated under 0 and 150 mM NaCl treatments. Results: A total of 172,986 mRNAs and 117,677 lncRNAs were identified, of which 2288 mRNAs and 1466 lncRNAs were significantly differentially expressed in four comparisons (GN5-150 vs. GN5-0, GN3-150 vs. GN3-0, GN5-0 vs. GN3-0, and GN5-150 vs. GN3-150). Based on GO and KEGG pathway analysis, the expression levels of several osmotic and ionic stress-related genes, including HPCA1-LRR, PP2C60, PP2C71, CRK1, APX3, HXK2, BAG6, and ARF1, were higher in GN5 than in GN3. CNGC1 was significantly downregulated by NaCl treatment in GN5. The co-expressed network analysis indicated that TCONS_00113549, TCONS_00399794, TCONS_00297228, TCONS_00004647, TCONS_00033214 and TCONS_00285177 in alfalfa roots regulated 66 genes including the four genes mentioned above. Therefore, the above nine genes and six lncRNAs might contribute to the difference in salinity tolerance between GN5 and GN3. Conclusions: We discovered a broad survey of mRNAs and lncRNAs associated with the difference of salinity tolerance between GN5 and GN3. These findings would be useful for further functional analysis as potential targets to improve resistance to salinity stress via genetic engineering.

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Heterologous expression of a novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene, ZjGRP, caused salt sensitivity in Arabidopsis

et al. 2016

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A novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene was cloned in this study and its overexpression caused salt sensitivity in transgenic Arabidopsis. Glycine-rich RNA-binding proteins (GRPs) play crucial roles in diverse plant developmental processes. However, the mechanisms and functions of GRPs in salinity stress responses remain largely unknown. In this study, rapid amplification of cDNA end (RACE) PCR methods was adopted to isolate ZjGRP from Zosyia japonica, a salt-tolerant grass species. ZjGRP cDNA was 456 bp in length, corresponding to 151 amino acids. ZjGRP was localized in the nucleus and cytoplasm, and was found particularly abundantly in stomatal guard cells. Quantitative real-time PCR showed that ZjGRP was expressed in the roots, stems, and leaves of Zoysia japonica, with the greatest expression seen in the fast-growing leaves. Furthermore, expression of ZjGRP was strongly induced by treatment with NaCl, ABA, MeJA, and SA. Overexpression of ZjGRP in Arabidopsis reduced the rate of germination and retarded seedling growth. ZjGRP-overexpressing Arabidopsis thaliana exhibited weakened salinity tolerance, likely as a result of effects on ion transportation, osmosis, and antioxidation. This study indicates that ZjGRP plays an essential role in inducing salt sensitivity in transgenic plants.

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Expression of an alfalfa (Medicago sativa L.) peroxidase gene in transgenic Arabidopsis thaliana enhances resistance to NaCl and H2O2

Cited by 10 publications

References 27 publications

Physiological status of high and low metabolism Hevea clones in the difference stage of tapping panel dryness

Physiological status of high and low metabolism Hevea clones in the difference stage of tapping panel dryness

Comparative physiology and transcriptome analysis to identify the important coding and non-coding RNAs imparting tolerance to salinity stress in alfalfa (Medicago sativa L.)

Heterologous expression of a novel Zoysia japonica salt-induced glycine-rich RNA-binding protein gene, ZjGRP, caused salt sensitivity in Arabidopsis

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