Oxidative stress, antioxidant activity and Fe(III)-chelate reductase activity of five Prunus rootstocks explants in response to Fe deficiency

Molassiotis, Athanassios; Diamantidis, Grigorios; Therios, Ioannis; Tsirakoglou, V.; Dimassi, K.

doi:10.1007/s10725-005-6396-z

Cited by 37 publications

(11 citation statements)

References 38 publications

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“…It is likely that these symptoms are linked to leaf chlorophyll and Fe concentrations as explained by Chen et al (). Analogous findings have also been reported in a broad range of crops such as peach (Molassiotis et al ), pear (Morales et al ), Chinese cabbage (Ding et al ), strawberry (Pestana et al ) and peanut (Zhang et al ). Exogenous MT enhanced chlorophyll content in the leaves of pepper exposed to Fe‐deficiency, but chlorophyll values were still lower than those in control plants (Fig.…”

Section: Discussionsupporting

confidence: 75%

Integrative roles of nitric oxide and hydrogen sulfide in melatonin‐induced tolerance of pepper (Capsicum annuum L.) plants to iron deficiency and salt stress alone or in combination

Kaya

Higgs

Ashraf

et al. 2019

Physiologia Plantarum

245

109

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There seems to be no report in the literature on the effect of melatonin (MT) in relieving the detrimental effects of combined application of salt stress (SS) and iron deficiency (ID). Therefore, the effect of MT on the accumulation/synthesis of endogenous nitric oxide (NO) and hydrogen sulphide (H2S) and how far these molecules are involved in MT‐improved tolerance to the combined application of ID and SS in pepper (Capsicum annuum L) were tested. Hence, two individual trials were set up. The treatments in the first experiment comprised: Control, ID (0.1 mM FeSO4), SS (100 mM NaCl) and ID + SS. The detrimental effects of combined stresses were more prominent than those by either of the single stress, with respect to growth, oxidative stress and antioxidant defense attributes. Single stress or both in combination improved the endogenous H2S and NO, and foliar‐applied MT (100 µM) led to a further increase in NO and H2S levels. In the second experiment, 0.1 mM scavenger of NO, 2‐(4‐carboxyphenyl)‐4,4,5,5‐tetramethylimidazoline‐1‐oxyl‐3‐oxide potassium salt (cPTIO) and that of H2S, hypotuarine (HT) were applied along with MT to get further evidence whether NO and H2S are involved in MT‐induced tolerance to ID and SS. MT combined with cPTIO and HT under a single or combined stress showed that NO effect was reversed by the NO scavenger, cPTIO, alone but the H2S effect was inhibited by both scavengers. These findings suggested that tolerance to ID and SS induced by MT may be involved in downstream signal crosstalk between NO and H2S.

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Section: Discussionsupporting

confidence: 75%

Integrative roles of nitric oxide and hydrogen sulfide in melatonin‐induced tolerance of pepper (Capsicum annuum L.) plants to iron deficiency and salt stress alone or in combination

Kaya

Higgs

Ashraf

et al. 2019

Physiologia Plantarum

245

109

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“…Bicarbonate can maintain a high pH (7.5–8.0) in the medium and, in this way, can affect Fe solubility and ferric reductase activity, since this latter one has an optimum pH around 5.0 (Römheld and Marschner , Moog and Brüggemann ). Nonetheless, and without discarding the importance of this pH buffer effect, there are some results showing that bicarbonate could also affect Fe nutrition by limiting the enhancement of ferric reductase activity and other Fe responses (Romera et al , Molassiotis et al , Lucena et al , ). The limitation of Fe responses by bicarbonate could be related to its negative effect on the expression of the transcription factors SlFER or AtFIT , and consequently on that of Fe acquisition genes, such as FRO , IRT and CsHA1 (Lucena et al ).…”

Section: Introductionmentioning

confidence: 99%

Hypoxia and bicarbonate could limit the expression of iron acquisition genes in Strategy I plants by affecting ethylene synthesis and signaling in different ways

García

García-Mateo

Lucena

et al. 2013

Physiologia Plantarum

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In a previous work, it was shown that bicarbonate (one of the most important factors causing Fe chlorosis in Strategy I plants) can limit the expression of several genes involved in Fe acquisition. Hypoxia is considered another important factor causing Fe chlorosis, mainly on calcareous soils. However, to date it is not known whether hypoxia aggravates Fe chlorosis by affecting bicarbonate concentration or by specific negative effects on Fe acquisition. Results found in this work show that hypoxia, generated by eliminating the aeration of the nutrient solution, can limit the expression of several Fe acquisition genes in Fe-deficient Arabidopsis, cucumber and pea plants, like the genes for ferric reductases AtFRO2, PsFRO1 and CsFRO1; iron transporters AtIRT1, PsRIT1 and CsIRT1; H(+) -ATPase CsHA1; and transcription factors AtFIT, AtbHLH38, and AtbHLH39. Interestingly, the limitation of the expression of Fe-acquisition genes by hypoxia did not occur in the Arabidopsis ethylene constitutive mutant ctr1, which suggests that the negative effect of hypoxia is related to ethylene, an hormone involved in the upregulation of Fe acquisition genes. As for hypoxia, results obtained by applying bicarbonate to the nutrient solution suggests that ethylene is also involved in its negative effect, since ACC (1-aminocyclopropane-1-carboxylic acid; ethylene precursor) partially reversed the negative effect of bicarbonate on the expression of Fe acquisition genes. Taken together, the results obtained show that hypoxia and bicarbonate could induce Fe chlorosis by limiting the expression of Fe acquisition genes, probably because each factor negatively affects different steps of ethylene synthesis and/or signaling.

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“…In our previous experiment, Fe deficiency significantly decreased chlorophyll concentration in peanut leaves . Such a large decrease in chlorophyll concentration can be ascribed to the role of Fe in the formation of δ-aminolevulinic acid and protochlorophyllide, the precursors of the chlorophyll biosynthesis (Molassiotis et al 2005). NO was suggested to be involved in plant responses triggered by Fe deficiency and to mediate Fe deficiency responses as a signal molecule (Leonor et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…These processes initiate chain reactions that produce more harmful oxygen radicals, which may destroy normal metabolism through oxidative damage of lipids, proteins, and nucleic acids when they are produced in excess (Molassiotis et al 2005). In addition, when plants were subjected to environmental stress, oxidative damage resulted in the level of membrane lipid peroxidation, which could be estimated by MDA content.…”

Section: Discussionmentioning

confidence: 99%

“…• − ) radicals in the electron transport to extracellular ferric chelates may be of significant importance for the acquisition of Fe by roots (Molassiotis et al 2005). In addition, Graziano and Lamattina (2005) proposed that the free radical NO as a candidate for the translation of the Fe deficiency signal and Zaharieva et al (2004) also suggested that responses to Fe deficiency may include a shift in the redox balance inside the root cell, along with an increased capacity of roots to reduce Fe.…”

Section: Discussionmentioning

confidence: 99%

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Role of exogenous nitric oxide in alleviating iron deficiency-induced peanut chlorosis on calcareous soil

Kong

Dong

et al. 2013

Journal of Plant Interactions

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This study examined the effects of exogenous nitric oxide (NO) on physiological characteristics of peanut (Arachis hypogaea L.) growing on calcareous soil. Sodium nitroprusside (SNP), a NO donor, was root application (directly; slow-release bag; slow-release capsule; slow-release particle) and foliar application. The results showed that SNP application alleviated iron (Fe) deficiency-induced chlorosis, increased the yield of peanut and increased the Fe concentration in peanut grain. SNP, especially supplied by slow-release particle improved the available Fe in soil by reducing pH of soil and increasing available Fe of soil. Furthermore, SNP application significantly increased the H + -ATPase and Fe 3 + reductase activities and increased the total Fe concentration in the leaves. Meanwhile, SNP application, especially foliar application enhanced the availability of Fe in the plant by significantly increasing the active Fe content and chlorophyll content in the leaves. In addition, SNP also increased the antioxidant activities, but decreased the superoxide anion (O 2• − ) generation rate and malondialdehyde content, which protected peanut against the Fe deficiency-induced oxidative stress. Therefore, these results support a physiological action of SNP on the availability, uptake and transport of Fe in the plant and foliar application SNP had the best effects in leaves and SNP supplied by slow-release particle had the best effects in roots. In addition, on the whole, the effects of SNP supplied by slow-release ways were better than directly supplied into the soil.

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Oxidative stress, antioxidant activity and Fe(III)-chelate reductase activity of five Prunus rootstocks explants in response to Fe deficiency

Cited by 37 publications

References 38 publications

Integrative roles of nitric oxide and hydrogen sulfide in melatonin‐induced tolerance of pepper (Capsicum annuum L.) plants to iron deficiency and salt stress alone or in combination

Integrative roles of nitric oxide and hydrogen sulfide in melatonin‐induced tolerance of pepper (Capsicum annuum L.) plants to iron deficiency and salt stress alone or in combination

Hypoxia and bicarbonate could limit the expression of iron acquisition genes in Strategy I plants by affecting ethylene synthesis and signaling in different ways

Role of exogenous nitric oxide in alleviating iron deficiency-induced peanut chlorosis on calcareous soil

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