Methanolic extract of Pereskia bleo (Kunth) DC. (Cactaceae) induces apoptosis in breast carcinoma, T47-D cell line

Tan, Mei Lan; Sulaiman, Shaida Fariza; Najimuddin, Nazalan; Samian, Mohammed Razip; Muhammad, Tengku Sifzizul Tengku

doi:10.1016/j.jep.2004.09.025

Cited by 93 publications

(75 citation statements)

References 31 publications

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“…[51], Arnebia nobilis [64], Cimicifuga foetida [65], Brassica oleracea [35], Calendula officinalis [25], Pereskia bleo (Kunth) DC. (Cactaceae) [61], and Catha edulis (Khat) [16].…”

Section: Mediummentioning

confidence: 99%

Anti-inflammatory, pro-apoptotic, and anti-proliferative effects of a methanolic neem (Azadirachta indica) leaf extract are mediated via modulation of the nuclear factor-κB pathway

et al. 2010

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Azadirachta indica (neem tree) is used in traditional Indian medicine for its pharmacological properties including cancer prevention and treatment. Here, we studied a neem extract's anti-inflammatory potential via the nuclear factor-jB (NF-jB) signaling pathway, linked to cancer, inflammation, and apoptosis. Cultured human leukemia cells were treated with a methanolic neem leaf extract with or without tumor necrosis factor (TNF)-a stimulation. Inhibition of NF-jB activity was demonstrated by luciferase assay and electrophoretic mobility shift assay (EMSA). Inhibition of viability by neem extracts was assessed by luminescent assays. Western blot analysis allowed assessing the inhibitory effect of the neem extract on TNF-a-induced degradation of inhibitor of jB (IjB) and nuclear translocation of the NF-jB p50/p65 heterodimer. Inhibition of IjB kinase (IKK) activity was shown as well as the effect of neem extract on the induction of apoptotic cell death mechanisms by nuclear fragmentation analysis and flow cytometry analysis. In conclusion, our data provide evidence for a strong effect of the neem extract on proinflammatory cell signaling and apoptotic cell death mechanisms, contributing to a better understanding of the mechanisms triggered by Azadirachta indica.

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“…[51], Arnebia nobilis [64], Cimicifuga foetida [65], Brassica oleracea [35], Calendula officinalis [25], Pereskia bleo (Kunth) DC. (Cactaceae) [61], and Catha edulis (Khat) [16].…”

Section: Mediummentioning

confidence: 99%

Anti-inflammatory, pro-apoptotic, and anti-proliferative effects of a methanolic neem (Azadirachta indica) leaf extract are mediated via modulation of the nuclear factor-κB pathway

et al. 2010

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“…Na Pereskia grandifolia, a vitamina E fitona e o 2,4-ditert-butilfenol (SRI NURESTRI et al, 2009) e na Pereskia bleo os compostos dihidroactinidiolide, 2,4-ditert-butilfenol, -tocopherol e fitol (SRI NURESTRI et al, 2008), apresentaram efeito citotóxico sobre as células carcinomas epidermoides nasofaríngea humana (KB), carcinoma cervical humano (CasKi), carcinoma do cólon humano (HCT116), carcinoma mamário dependente de hormônio (MCF7) e carcinoma de pulmão humano (A549). TAN et al (2005) observaram significante atividade citotóxica das folhas de Pereskia bleo sobre as linhagens T47D. ER et al (2007) observaram que o extrato aquoso e metanólico das folhas de Pereskia bleo não apresentaram efeito significativo antiproliferativo contra as células neoplásicas mamárias de ratos (4T1), não sendo condizente com o argumento utilizado por comunidades da Malásia, em que as folhas são utilizadas para o tratamento do câncer.…”

Section: Resultsunclassified

Utilização de cactáceas do gênero Pereskia na alimentação humana em um município de Minas Gerais

Almeida

Corrêa

2012

Cienc. Rural

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“…This is a well-recognized criterion for a compound to be judged as cytotoxic, as defined by the National Cancer Institute (NCI). [11] In the present study the growth passages of the HepG 2 cells that were used in all experiments were between4-7 to avoid advanced passages that may has lost their parental characteristics or morphology, thereby producing unreliable data. [12] Water decreases the viability of HepG 2 liver cancer cell line.…”

Section: Serial Dilution Of Methanol Extractmentioning

confidence: 99%

The Anti-Proliferative Activity of Vitis Vinifera Leaves of Water Extract Alone and in Combination With Doxorubicin Against Liver Cancer Cell Line.

Ali¹

2017

WJPR

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The aim of this study was to investigate the capability of Vitis vinifera leaves of water extracts alone and in combination with doxorubicin to inhibit HepG2 liver cancer cell line proliferation and to identify the possible mechanism of action. The anti proliferative activity of the water extracts tested by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay on liver cancer cell line (HepG2). Dose response relationship was shown. Significant cytotoxic activity of water extracts has identified on HepG2, the IC 50 was 20.8μg/ml. When in combination with doxorubicin Dose response relationship was shown. No significant cytotoxic activity of the water extracts combination; the IC 50 was 31.5μg/ml. These finding showed that water extracts combination did not show any cytotoxic action at the applied dose, so no toxic effect against the HepG2 cell line can be expected in vitro (contrast action).

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Methanolic extract of Pereskia bleo (Kunth) DC. (Cactaceae) induces apoptosis in breast carcinoma, T47-D cell line

Cited by 93 publications

References 31 publications

Anti-inflammatory, pro-apoptotic, and anti-proliferative effects of a methanolic neem (Azadirachta indica) leaf extract are mediated via modulation of the nuclear factor-κB pathway

Anti-inflammatory, pro-apoptotic, and anti-proliferative effects of a methanolic neem (Azadirachta indica) leaf extract are mediated via modulation of the nuclear factor-κB pathway

Utilização de cactáceas do gênero Pereskia na alimentação humana em um município de Minas Gerais

The Anti-Proliferative Activity of Vitis Vinifera Leaves of Water Extract Alone and in Combination With Doxorubicin Against Liver Cancer Cell Line.

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