The Anti-Proliferative Activity of Vitis Vinifera Leaves of Water Extract Alone and in Combination With Doxorubicin Against Liver Cancer Cell Line.

Abstract

The aim of this study was to investigate the capability of Vitis vinifera leaves of water extracts alone and in combination with doxorubicin to inhibit HepG2 liver cancer cell line proliferation and to identify the possible mechanism of action. The anti proliferative activity of the water extracts tested by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay on liver cancer cell line (HepG2). Dose response relationship was shown. Significant cytotoxic activity of water extracts has identified on HepG2, the IC 50 was 20.8μg/ml. When in combination with doxorubicin Dose response relationship was shown. No significant cytotoxic activity of the water extracts combination; the IC 50 was 31.5μg/ml. These finding showed that water extracts combination did not show any cytotoxic action at the applied dose, so no toxic effect against the HepG2 cell line can be expected in vitro (contrast action).

Antioxidant, antimicrobial, and anticancer activity of an extract of two pollution-tolerant green microalgae

“…respectively. A significant cytotoxic effect against the HepG2 an d WI-38 cellline is suggested via an IC50 value of less tha n 100 μg/mL [49], and this implies that the two have powerful cytotoxi c impacts on the HepG2 cell line. It can be said that extract Tetradesmus obliquus has a more cytotoxic effect with 36.44 μg/mL IC50 value.…”