Metalloproteinase 14 (MMP-14) and hsa-miR-410-3p expression in human inflamed dental pulp and odontoblasts

Abstract

The objective of this study is to evaluate MMP-14 expression in odontoblasts and in the bulk of dental pulp of teeth with pulpitis; to determine the expression of microRNA-410 (miR-410) in pulp tissue, since sequence analysis suggests that miR-410 has potential binding site on MMP-14’s 3′UTR, and hence, can regulate expression of the latter one. Tissue samples of dental pulp from teeth with pulpitis and healthy (control) were formalin fixed and paraffin embedded (FFPE). Samples were examined using immunohistochemical staining for MMP-14 and the expression of miR-410 was evaluated using qRT-PCR. In both, healthy and inflamed pulp odontoblasts stained more intensively than remaining pulp tissue, but this difference was not statistically significant. More positive staining was observed in inflamed pulps compared to healthy pulps. Expression of miR-410 was found significantly lower in inflamed pulps than in healthy ones. In the two examined zones, odontoblasts and remaining pulp, miR-410 was expressed on a similar level. No statistically significant correlation of miR-410 and MMP-14 expression was found. We showed that inflammation changes the MMP-14 expression in pulp tissue and odontoblasts. This study demonstrates for the first time miR-410 expression in human dental pulp and that expression of this microRNA was downregulated in inflamed dental pulp and odontoblasts.

Deciphering Black Extrinsic Tooth Stain Composition in Children Using Metaproteomics

“…Our findings show also that cathepsin (one of the host-induced enzymes that are intensely involved within caries development) is uniquely identified in BS category . Furthermore, our results show the unique identification of metalloproteinase in BS, and this matches with what was described earlier that metalloproteinase is promoting the formation of caries/BSs …”

Matrix Metalloproteinases in Dental and Periodontal Tissues and Their Current Inhibitors: Developmental, Degradational and Pathological Aspects

“…Cysteine cathepsin of dentine is able to activate latent MMPs. At increasing depth of carious lesion, cathepsin activity becomes stronger with greater collagenolytic potential as more MMPs become activated [ 45 ]. Regardless of the rate of progress of dental caries, endogenous dentine MMPs decrease with aging [ 4 ].…”

“…On the other hand, there is a more increased release of active MMPs in pulpitis than in healthy pulp tissue, indicating their role in pulp inflammation: released cytokines (IL-1β) and tumor necrosis factor-α (TNF-α) in pulp inflammation, activate MMP-1, MMP-2 and TIMP1 gene expression [ 45 ]. While MMP-2 expression was observed in the dental papilla cells, dental follicle, ameloblasts, odontoblasts and bone cells from the coronal and basal regions of the bony crypt [ 30 ], bacteroids and anaerobic bacteria can also stimulate excretion of MMP-1, MMP-2 and TIMP1 by the pulp cells [ 45 ]. The level of MMP-2 in root canal exudate of teeth with pulp necrosis or asymptomatic apical periodontitis is reduced gradually with root canal treatment procedures, which might validate MMP-2 as a biomarker [ 57 ].…”

Approaches Toward Targeting Matrix Metalloproteases for Prognosis and Therapies in Gynecological Cancer: MicroRNAs as a Molecular Driver

“…showed a higher MMP-14 expression in endometrial adenocarcinoma tissue with a decrease in miR-410 level, suggesting a regulatory effect of miR-410 in modulating EC cell progression although the mechanism is largely unknown ( 13 ). Studies in odontoblast cells suggest the presence of a probable binding site for miR-410 on 3’UTR of MMP-14 ( 82 ). In lung cancer, miR-410 has a tumor-suppressive role by inducing apoptosis through downregulating JAK/STAT3/SOCS3 signaling pathway ( 113 ).…”