Deciphering Black Extrinsic Tooth Stain Composition in Children Using Metaproteomics

Hirtz, Christophe; Mannaa, Atef; Moulis, Estelle; Pible, Olivier; O’flynn, Robin; Armengaud, Jean; Jouffret, Virginie; Lemaistre, Camille; Dominici, Gabriel; Martinez, Alex Yahiaoui; Dunyach-Rémy, Catherine; Tiers, Laurent; Lavigne, Jean‐Philippe; Tramini, Paul; Goldsmith, Marie-christine; Lehmann, S.; Périère, Dominique Deville de; Vialaret, Jérôme

doi:10.1021/acsomega.1c04770

Cited by 6 publications

(2 citation statements)

References 40 publications

(75 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…NanoLC-MS/MS analyses were conducted using a Q-Exactive HF tandem mass spectrometer (Thermo Fisher Scientific) in data-dependent mode with a 60 min gradient acquisition as described . Proteotyping was performed as previously described. , Briefly, a database derived from the National Center for Biotechnology Information nonredundant (NCBInr) database and comprising only a restricted number of representatives per species as presented earlier was queried using Mascot Daemon version 2.6.1 search engine (Matrix Science). A second database comprising all of the identified genera from the first round search and their descendants was then created and searched for.…”

Section: Methodsmentioning

confidence: 99%

Label-Free Multiplex Proteotyping of Microbial Isolates

Chabas,

Pible,

Armengaud

et al. 2023

Anal. Chem.

Self Cite

View full text Add to dashboard Cite

To meet clinical diagnostic needs and for general microbiological screening, it is essential to be able to accurately and rapidly identify any microorganisms from complex microbiota. To gain insight into the individual components of microbiota, culturomics has been proposed as a means to systematically test hundreds of possible cultivation conditions and generate numerous microbial isolates with very distinct characteristics. Highthroughput identification methods must now be developed to quickly screen these isolates. Currently, most multiplexing methods involve labeling, which comes at a cost. In this paper, we present an innovative label-free multiplexing method for the identification of microorganisms using tandem mass spectrometry. The method is based on offline reversed-phase fractionation of individual peptidomes. Multiplexing is achieved by mixing fractions of staged hydrophobicity; thus, each sample is mapped to specific elution times. In this proof-of-concept study, multiplexed samples were analyzed by tandem mass spectrometry in a single run and microorganisms present in the mixture were resolved by phylopeptidomics proteotyping. Using this methodology, up to 21 microorganisms could be identified in a single 60 min run performed with a Q-Exactive HF high-resolution mass spectrometer, resulting in a rate of one microorganism identified per 3 min of mass spectrometry, without any need for the use of labeling reagents. This approach opens new perspectives for the application of high-throughput proteotyping of bacteria using tandem mass spectrometry in large culturomics projects.

show abstract

Section: Methodsmentioning

confidence: 99%

Label-Free Multiplex Proteotyping of Microbial Isolates

Chabas,

Pible,

Armengaud

et al. 2023

Anal. Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Following migration, one gel was transferred onto a PVDF membrane and hybridised with the mouse monoclonal anti-CRALBP antibody. The second gel was rinsed and stained in Coomassie blue staining solution (InstantBlue, Expedeon) for 1 h. The bands of interest were cut from the Coomassie stained gel using the western blot filter as a guide and an in-gel digestion using a protease (Sequencing grade Trypsin, Promega) was performed prior to proteomic analysis by nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS) using a QTOF Impact II (Bruker Daltonics) mass spectrometer, as described (59).…”

Section: Proteomic Analysismentioning

confidence: 99%

Dual CRALBP isoforms unveiled: iPSC-derived retinal modelling and AAV2/5-RLBP1 gene transfer raise considerations for effective therapy

Damodar,

Dubois,

Guillou

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Inherited retinal diseases (IRDs) are clinically and genetically heterogeneous disorders characterised by progressive vision loss. Over 270 causative genes have been identified and variants within the same gene can give rise to clinically distinct disorders. Human induced pluripotent stem cells (iPSCs) have revolutionised disease modelling, by allowing pathophysiological and therapeutic studies in the patient and tissue context. The IRD gene RLBP1 encodes CRALBP, an actor of the rod and cone visual cycles in the retinal pigment epithelium (RPE) and Muller cells, respectively. Variants in RLBP1 lead to three clinical subtypes: Bothnia dystrophy, Retinitis punctata albescens and Newfoundland rod-cone dystrophy. We modelled RLBP1-IRD subtypes by patient-specific iPSC-derived RPE and identified pertinent therapeutic read-outs. We developed an AAV2/5-mediated gene replacement strategy and provided a proof-of-concept in the ex vivo human models that was validated in an in vivo Rlbp1-/- murine model. Most importantly, we identified a previously unsuspected smaller CRALBP isoform that is naturally and differentially expressed in both human and murine retina. The new isoform arises from an alternative methionine initiation site and plays a role in the visual cycle. This work provides novel insights into CRALBP expression and RLBP1-associated pathophysiology and raises important considerations for successful gene supplementation therapy.

show abstract

Oral microbial profiles of extrinsic black tooth stain in primary dentition: A literature review

Dong,

Zhao,

et al. 2024

Journal of Dental Sciences

View full text Add to dashboard Cite

Deciphering Black Extrinsic Tooth Stain Composition in Children Using Metaproteomics

Cited by 6 publications

References 40 publications

Label-Free Multiplex Proteotyping of Microbial Isolates

Label-Free Multiplex Proteotyping of Microbial Isolates

Dual CRALBP isoforms unveiled: iPSC-derived retinal modelling and AAV2/5-RLBP1 gene transfer raise considerations for effective therapy

Oral microbial profiles of extrinsic black tooth stain in primary dentition: A literature review

Contact Info

Product

Resources

About