Metabolomics for laboratory diagnostics

Buják, Renata; Struck-Lewicka, Wiktoria; Markuszewski, Michał J.; Kaliszan, Roman

doi:10.1016/j.jpba.2014.12.017

Cited by 291 publications

(239 citation statements)

References 94 publications

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“…Metabolomics aims to identify and (semi-)quantify the small molecule metabolites present in a studied sample [1]. This approach has been extensively applied in biomedical research to find disease biomarkers [1], study therapeutic effects of drugs or natural substances with potential therapeutic capabilities [2], or explore metabolic pathways perturbed by a particular disease or condition [3].…”

Section: Introductionmentioning

confidence: 99%

LC-MS-Based Metabolic Fingerprinting of Aqueous Humor

Pietrowska

Dmuchowska

Samczuk

et al. 2017

Journal of Analytical Methods in Chemistry

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Aqueous humor (AH) is a transparent fluid which fills the anterior and posterior chambers of the eye. It supplies nutrients and removes metabolic waste from avascular tissues in the eye. Proper homeostasis of AH is required to maintain adequate intraocular pressure as well as optical and refractive properties of the eye. Application of metabolomics to study human AH may improve knowledge about the molecular mechanisms of eye diseases. Until now, global analysis of metabolites in AH has been mainly performed using NMR. Among the analytical platforms used in metabolomics, LC-MS allows for the highest metabolome coverage. The aim of this study was to develop a method for extraction and analysis of AH metabolites by LC-QTOF-MS. Different protocols for AH preparation were tested. The best results were obtained when one volume of AH was mixed with one volume of methanol : ethanol (1 : 1). In the final method, 2 µL of extracted sample was analyzed by LC-QTOF-MS. The method allowed for reproducible measurement of over 1000 metabolic features. Almost 250 metabolites were identified in AH and assigned to 47 metabolic pathways. This method is suitable to study the potential role of amino acids, lipids, oxidative stress, or microbial metabolites in development of ocular diseases.

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Section: Introductionmentioning

confidence: 99%

LC-MS-Based Metabolic Fingerprinting of Aqueous Humor

Pietrowska

Dmuchowska

Samczuk

et al. 2017

Journal of Analytical Methods in Chemistry

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show abstract

“…Metabolomics refers to the systematic identification and quantification (or semi-quantification) of the small molecule metabolites present in a biological system (cell, tissue, organ, biological fluid, or organism) at a specific point in time [76]. This latest -omics platform is based on high throughput analytical techniques and biostatistical tools.…”

Section: Analytical Tools For Metabolomicsmentioning

confidence: 99%

“…Methods used for this step include liquid chromatography (LC), gas chromatography (GC) and capillary electrophoresis (CE) [82]. Considering complexity of the metabolome none of available technologies is able to provide information about complete set of metabolites in biological fluid or tissue [76]. Therefore all methodologies provide complementary information and to increase metabolites coverage in the sample of interest more than one platform should be used.…”

Section: Analytical Tools For Metabolomicsmentioning

confidence: 99%

“…CE-MS is suitable for polar and charged molecules while GC-MS for volatile and thermally stable analytes (time consuming derivatization step is required to achieve more metabolites with such properties). LC-MS is more versatile technique and depending on the type of column less polar and non-polar (reversed phase chromatography) or polar (hydrophilic interactions or ion-exchange chromatography) metabolites can be measured [76].…”

Section: Analytical Tools For Metabolomicsmentioning

confidence: 99%

“…Both strategies require initial hypothesis about metabolite or group of metabolites which might be altered in response to studied parameter (e.g. disease, pharmacotherapy, intervention) [76]. However, the greatest potential for discovery of novel findings has metabolic fingerprinting.…”

Section: Analytical Tools For Metabolomicsmentioning

confidence: 99%

See 2 more Smart Citations

Metabolomics — A wide-open door to personalized treatment in chronic heart failure?

Marcinkiewicz-Siemion

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Discovery and identification of potential biomarkers for alcohol‐induced oxidative stress based on cellular metabolomics

Wei

Fei

et al. 2017

Biomedical Chromatography

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Biomarkers involved in alcohol-induced oxidative stress play an important role in alcoholic liver disease prevention and diagnosis. Alcohol-induced oxidative stress in human liver L-02 cells was used to discover the potential biomarkers. Metabolites from L-02 cells induced by alcohol were measured by high-performance liquid chromatography and mass spectrometry. Fourteen metabolites that allowed discrimination between control and model groups were discovered by multivariate statistical data analysis (i.e. principal components analysis, orthogonal partial least-squares discriminate analysis). Based on the retention time, UV spectrum and LC-MS findings of the samples and compared with the authentic standards, eight biomarkers involved in alcohol-induced oxidative stress, namely, malic acid, oxidized glutathione, γ-glutamyl-cysteinyl-glycine, adenosine triphosphate, phenylalanine, adenosine monophosphate, nitrotyrosine and tryptophan, were identified. These biomarkers offered important targets for disease diagnosis and other researches.

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Metabolomics for laboratory diagnostics

Cited by 291 publications

References 94 publications

LC-MS-Based Metabolic Fingerprinting of Aqueous Humor

LC-MS-Based Metabolic Fingerprinting of Aqueous Humor

Metabolomics — A wide-open door to personalized treatment in chronic heart failure?

Discovery and identification of potential biomarkers for alcohol‐induced oxidative stress based on cellular metabolomics

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