Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses

Abstract

Globally, many crop production areas are threatened by drought and salinity. Potato (Solanum tuberosum L.) is susceptible to these challenging environmental conditions. In this study, an in vitro approach was employed to compare the tolerance of potato cultivars ‘BARI-401’ (red skin) and ‘Spunta’ (yellow skin). To simulate ionic and osmotic stress, MS media was supplemented with lithium chloride (LiCl 20 mM) and mannitol (150 mM). GC-MS and spectrophotometry techniques were used to determine metabolite accumulation. Other biochemical properties, such as total phenols concentration (TPC), total flavonoids concentration (TFC), antioxidant capacity (DPPH free radical scavenging capacity), polyphenol oxidase (PPO), and peroxidase (POD) activities, were also measured. The two cultivars respond differently to ionic and osmotic stress treatments, with Spunta accumulating more defensive metabolites in response, indicating a higher level of tolerance. While further investigation of the physiological and biochemical responses of these varieties to drought and salinity is required, the approach taken in this paper provides useful information prior to open field evaluation.

Transcriptome-based analysis of salt-related genes during the sprout stage of common bean (Phaseolus vulgaris) under salt stress conditions

“…The fact that oxidoreductase activity was included among the enriched GO terms is consistent with the results of earlier research on Antarctic mosses under salt stress conditions [59] and Caragana korshinskii seedlings [60]. The SOD, POD and CAT activity levels have been applied as physiological indicators of oxidoreductase activity to evaluate plants exposed to salt stress [61][62][63]. The results of this study indicated that oxidoreductase activities differed substantially between N and R in response to salt stress.…”

Early Growth Stage Characterization and the Biochemical Responses for Salinity Stress in Tomato

“…For our result with these three tomato genotypes with varying salt tolerance level, we can conclude that the particular phenolic compound was not reactive in the present reaction. Similar biochemical responses were also observed in potato [71].…”

Growth and Biochemical Responses of Potato Cultivars under In Vitro Lithium Chloride and Mannitol Simulated Salinity and Drought Stress

“…The increasing POD activity in both cultivars was examined in this study by being placed in LiCl and mannitol treatments, with Spunta increasing at lower mannitol levels suggesting that an earlier stress response may allow it to respond more efficiently than BARI-401 to drought stress. This has been reported by us previously in a study where Spunta was found to respond differently than BARI-401 when treated with 20 mM of LiCl and 150 mM of mannitol with several biochemical and metabolomic profile changes [44]. Similarly, POD activity has been observed to increase in potato genotypes Agria, Diament, Kennebec, and Ajax when exposed to 50 mM of NaCl, while POD and CAT contribute to a ROS detoxification [58].…”

“…Similarly, assessing the TPC in two species of Achillea under drought found the contents to be increased under drought conditions [ 43 ]. Previously, we showed that the TPC were not altered under moderate LiCl and mannitol levels in BARI-401 and Spunta [ 44 ].…”

“…Root length (cm) was measured from stem to root tip. Potato shoots (2 g sample) were selected at random, and extraction was done according to [44]. Then, 20 mL of 80% methanol was mixed with the sample and shaken at 150 rpm for 12 h and was then filtered through Whatman filter paper No.…”