Transcriptome-based analysis of salt-related genes during the sprout stage of common bean (Phaseolus vulgaris) under salt stress conditions

Abstract

a cereals germplasm Resources innovation laboratory, college of agriculture, national coarse cereals engineering Research center, heilongjiang Bayi agricultural university, Daqing, heilongjiang, pR china; b Bean crops laboratory, crop Resources institute of heilongjiang academy of agricultural Sciences, harbin, heilongjiang, pR china; c cereals germplasm Resources innovation laboratory, college of agriculture, national coarse cereals engineering Research center, Daqing, heilongjiang, pR china ABSTRACT Salt stress is an important factor restricting crop growth. Thus, genes related to plant responses to high salinity at the sprout stage of common bean (Phaseolus vulgaris) should be identified and characterized. In this study, on the basis of the common bean germination index, 0.4% NaCl and day 5 of the incubation period were selected as the optimal salt concentration and sampling time, respectively. Salt-tolerant and salt-sensitive plant materials suitable for the local region were selected as representative common bean materials with contrasting salt-related phenotypes. A total of 441 differentially expressed genes (DEGs) were identified by analyzing the generated RNA-seq data. The enriched Gene Ontology terms and Kyoto Encyclopedia of Genes and Genomes pathways indicated the DEGs were associated with oxidoreductase activities and phenylpropanoid biosynthesis. Furthermore, the superoxide dismutase, peroxidase, and catalase activities and the lignin content, which are related to oxidoreductase activities and phenylpropanoid biosynthesis, were revealed to be significantly associated with common bean salt tolerance. The six DEGs with salt tolerance-related Arabidopsis thaliana homologs may be important candidate genes mediating the salt stress responses of common bean during the sprout stage.

Molecular mechanisms of flavonoid accumulation in germinating common bean (Phaseolus vulgaris) under salt stress

“…As common abiotic stress, salt stress is important and accelerates the accumulation of certain plant metabolites ( 50 , 51 ). Several studies have demonstrated that 70 mmol/L NaCl is the appropriate salt concentration for inducing salinity stress in the common bean ( 22 , 52 ). Rutin is an important class of secondary metabolites widely found in plants and contributes to plant growth and development ( 16 ).…”

“…In contrast, stress might inhibit plant germination and reduce flavonoid accumulation. The salt-tolerant common bean germinated better than the salt-sensitive variety, while oxidase enzyme activities of salt-sensitive variety produced insignificant differences at the sprouting stage, limiting plant growth and vitality ( 52 ). Here, short-term salt stress was more conducive to rutin accumulation in germinating common beans, and 3d+S could be used to study the mechanism of rutin accumulation under salt stress.…”

“…Blue to red color represents expression from low to high. demonstrated that 70 mmol/L NaCl is the appropriate salt concentration for inducing salinity stress in the common bean (22,52). Rutin is an important class of secondary metabolites widely found in plants and contributes to plant growth and development (16).…”

RNA-Seq Analysis Demonstrates Different Strategies Employed by Tiger Nuts (Cyperus esculentus L.) in Response to Drought Stress

“…And then RNA was extracted by RNApure Plant Kit (CW0559, CWBIO, Beijing, China) following the manufacturer's instructions. The RNA was measured in 1% agarose gel electrophoresis and NanoDrop instrumentation (OneC, Thermo, Waltham, MA, USA) using the methods of Zhang [20], which determined whether the quality of the RNA was qualified. The qualified RNA of these samples underwent RNA-Seq by Bio-maker (Beijing, China), while those with no reference genome were analyzed by Biocloud (https://international.biocloud.net/, accessed on 1 January 2022).…”

“…ABA could induce the starch and sucrose metabolism to enhance stress tolerance [ 19 ]. Although drought stress affects all plant growth and development stages, the bud stage is the most sensitive and affects subsequent plant growth periods [ 20 ].…”