2007
DOI: 10.1007/s00248-006-9133-x
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Metabolic Activity and Phylogenetic Diversity of Reed (Phragmites australis) Periphyton Bacterial Communities in a Hungarian Shallow Soda Lake

Abstract: In the present study, the species composition and potential metabolic activities of bacterial communities of reed Phragmites australis (Cav.) (Trin. ex Steudel) periphyton from Lake Velencei were studied by cultivation-based and metabolic fingerprinting methods. Serially diluted spring biofilm samples were used to test the community-level physiological profiling (CLPP) using BIOLOG microplates, and for plating onto different media. On the basis of their morphological, biochemical, and physiological test result… Show more

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Cited by 27 publications
(16 citation statements)
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“…Final DNA elution was made into 100 ll distilled water. Partial 16S rDNA was amplified from 3 ll purified genomic DNA by PCR using universal bacterial 16S rDNA primers 338f (Suzuki & Giovannoni 1996) and 519r (Lane, 1991) in a Biometra T Personal thermocycler (Biometra Biomedizinische Analytik GmbH, Goettingen, Germany) as described by Borsodi et al (2007) with the following modification: the first denaturation step was carried out at 98°C for 5 min. A GC-clamp (5 0 CGCCCGCCGCGCGCGGCGGGC GGGGCGGGGCAGGGGGG 3 0 ) was connected to the 5 0 end of the 338f primer.…”
Section: Samplingmentioning
confidence: 99%
“…Final DNA elution was made into 100 ll distilled water. Partial 16S rDNA was amplified from 3 ll purified genomic DNA by PCR using universal bacterial 16S rDNA primers 338f (Suzuki & Giovannoni 1996) and 519r (Lane, 1991) in a Biometra T Personal thermocycler (Biometra Biomedizinische Analytik GmbH, Goettingen, Germany) as described by Borsodi et al (2007) with the following modification: the first denaturation step was carried out at 98°C for 5 min. A GC-clamp (5 0 CGCCCGCCGCGCGCGGCGGGC GGGGCGGGGCAGGGGGG 3 0 ) was connected to the 5 0 end of the 338f primer.…”
Section: Samplingmentioning
confidence: 99%
“…Earlier studies have focused on cell counts and the activity of bacteria in the reed rhizosphere using cultivation-based techniques (Borsodi et al, 2003). Others have focused on the community structure and diversity of bacteria associated with the reed rhizosphere in freshwaters using molecular methods (Borsodi et al, 2007;Ravit et al, 2007;Rusznyak et al, 2007;Vladar et al, 2008), but no study has examined the endophytic bacteria associated with reed roots and their possible roles in phytoremediation mediated by reed wetland.…”
Section: Introductionmentioning
confidence: 99%
“…In the soda lake (Borsodi et al, 2007) and alkaline lake (Joshi et al, 2008), only three phyla were found; Proteobacteria, Actinobacteria, and Firmicutes, but phylum Bacteroidetes was not detected. In the wetland of East Calcutta, 4 phyla were found; Proteobacteria, Actinobacteria, and Firmicutes, and Bacteroidetes, but, subclasses Alphaproteobacteria and Betaproteobacteria were not detected (Ghosh et al, 2007).…”
mentioning
confidence: 75%
“…Previous studies on the bacterial diversity were focused on either the isolates or cultivation-independent 16S rRNA gene clone library (Humayoun et al, 2003;Borsodi et al, 2007;Ghosh et al, 2007;Joshi et al, 2008). In this study, comprehensive study on the bacterial community structure of the Woopo wetland was performed on the isolates and cultivation-independent 16S rRNA gene clone library.…”
mentioning
confidence: 99%