Endophytic bacterial diversity in roots of Phragmites australis in constructed Beijing Cuihu Wetland (China)

Li, Yan Hong; Zhu, Jin; Zhai, Zhen Hua; Zhang, Qiang

doi:10.1111/j.1574-6968.2010.02015.x

Cited by 46 publications

(34 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All of them were sulfate-reducing bacteria, and their distributions were influenced by salinity and plant nutrient [45]. They played important roles in the metabolism of nitrogen, phosphorus, sulfur, and some organic compounds in wetland systems [18, 46]. Anaeromyxobacter was also the genus owning more than 1.0% proteobacterial sequences.…”

Section: Resultsmentioning

confidence: 99%

A Meta-Analysis of the Bacterial and Archaeal Diversity Observed in Wetland Soils

et al. 2014

The Scientific World Journal

View full text Add to dashboard Cite

This study examined the bacterial and archaeal diversity from a worldwide range of wetlands soils and sediments using a meta-analysis approach. All available 16S rRNA gene sequences recovered from wetlands in public databases were retrieved. In November 2012, a total of 12677 bacterial and 1747 archaeal sequences were collected in GenBank. All the bacterial sequences were assigned into 6383 operational taxonomic units (OTUs 0.03), representing 31 known bacterial phyla, predominant with Proteobacteria (2791 OTUs), Bacteroidetes (868 OTUs), Acidobacteria (731 OTUs), Firmicutes (540 OTUs), and Actinobacteria (418 OTUs). The genus Flavobacterium (11.6% of bacterial sequences) was the dominate bacteria in wetlands, followed by Gp1, Nitrosospira, and Nitrosomonas. Archaeal sequences were assigned to 521 OTUs from phyla Euryarchaeota and Crenarchaeota. The dominating archaeal genera were Fervidicoccus and Methanosaeta. Rarefaction analysis indicated that approximately 40% of bacterial and 83% of archaeal diversity in wetland soils and sediments have been presented. Our results should be significant for well-understanding the microbial diversity involved in worldwide wetlands.

show abstract

Section: Resultsmentioning

confidence: 99%

A Meta-Analysis of the Bacterial and Archaeal Diversity Observed in Wetland Soils

et al. 2014

The Scientific World Journal

View full text Add to dashboard Cite

show abstract

“…Plant roots were washed with tap water to remove soil. Surface sterilization of roots was performed according to Li et al (2010), with slight modifications. In this study, 2% sodium hypochlorite and sterile DEPC-treated water were used.…”

Section: Methodsmentioning

confidence: 99%

The Effects of Cropping Regimes on Fungal and Bacterial Communities of Wheat and Faba Bean in a Greenhouse Pot Experiment Differ between Plant Species and Compartment

et al. 2017

View full text Add to dashboard Cite

Many bacteria and fungi in the plant rhizosphere and endosphere are beneficial to plant nutrient acquisition, health, and growth. Although playing essential roles in ecosystem functioning, our knowledge about the effects of multiple cropping regimes on the plant microbiome and their interactions is still limited. Here, we designed a pot experiment simulating different cropping regimes. For this purpose, wheat and faba bean plants were grown under controlled greenhouse conditions in monocultures and in two intercropping regimes: row and mixed intercropping. Bacterial and fungal communities in bulk and rhizosphere soils as well as in the roots and aerial plant parts were analyzed using large-scale metabarcoding. We detected differences in microbial richness and diversity between the cropping regimes. Generally, observed effects were attributed to differences between mixed and row intercropping or mixed intercropping and monoculture. Bacterial and fungal diversity were significantly higher in bulk soil samples of wheat and faba bean grown in mixed compared to row intercropping. Moreover, microbial communities varied between crop species and plant compartments resulting in different responses of these communities toward cropping regimes. Leaf endophytes were not affected by cropping regime but bacterial and fungal community structures in bulk and rhizosphere soil as well as fungal community structures in roots. We further recorded highly complex changes in microbial interactions. The number of negative inter-domain correlations between fungi and bacteria decreased in bulk and rhizosphere soil in intercropping regimes compared to monocultures due to beneficial effects. In addition, we observed plant species-dependent differences indicating that intra- and interspecific competition between plants had different effects on the plant species and thus on their associated microbial communities. To our knowledge, this is the first study investigating microbial communities in different plant compartments with respect to multiple cropping regimes using large-scale metabarcoding. Although a simple design simulating different cropping regimes was used, obtained results contribute to the understanding how cropping regimes affect bacterial and fungal communities and their interactions in different plant compartments. Nonetheless, we need field experiments to properly quantify observed effects in natural ecosystems.

show abstract

“…About 3 g of plant material, approximately more than one thousand of entire plants was collected after absorbing enough water, and then mixed together and immediately transported to the laboratory for surface disinfection as described previously (Li et al , 2010). The plants were first washed many times with tap water to remove attached substratum.…”

Section: Methodsmentioning

confidence: 99%

A primary assessment of the endophytic bacterial community in a xerophilous moss (Grimmia montana) using molecular method and cultivated isolates

Liu¹,

Liu²,

Liu³

et al. 2014

Braz. J. Microbiol.

Self Cite

View full text Add to dashboard Cite

Investigating the endophytic bacterial community in special moss species is fundamental to understanding the microbial-plant interactions and discovering the bacteria with stresses tolerance. Thus, the community structure of endophytic bacteria in the xerophilous moss Grimmia montana were estimated using a 16S rDNA library and traditional cultivation methods. In total, 212 sequences derived from the 16S rDNA library were used to assess the bacterial diversity. Sequence alignment showed that the endophytes were assigned to 54 genera in 4 phyla (Proteobacteria, Firmicutes, Actinobacteria and Cytophaga/Flexibacter/Bacteroids). Of them, the dominant phyla were Proteobacteria (45.9%) and Firmicutes (27.6%), the most abundant genera included Acinetobacter, Aeromonas, Enterobacter, Leclercia, Microvirga, Pseudomonas, Rhizobium, Planococcus, Paenisporosarcina and Planomicrobium. In addition, a total of 14 species belonging to 8 genera in 3 phyla (Proteobacteria, Firmicutes, Actinobacteria) were isolated, Curtobacterium, Massilia, Pseudomonas and Sphingomonas were the dominant genera. Although some of the genera isolated were inconsistent with those detected by molecular method, both of two methods proved that many different endophytic bacteria coexist in G. montana. According to the potential functional analyses of these bacteria, some species are known to have possible beneficial effects on hosts, but whether this is the case in G. montana needs to be confirmed.

show abstract

Endophytic bacterial diversity in roots of Phragmites australis in constructed Beijing Cuihu Wetland (China)

Cited by 46 publications

References 46 publications

A Meta-Analysis of the Bacterial and Archaeal Diversity Observed in Wetland Soils

A Meta-Analysis of the Bacterial and Archaeal Diversity Observed in Wetland Soils

The Effects of Cropping Regimes on Fungal and Bacterial Communities of Wheat and Faba Bean in a Greenhouse Pot Experiment Differ between Plant Species and Compartment

A primary assessment of the endophytic bacterial community in a xerophilous moss (Grimmia montana) using molecular method and cultivated isolates

Contact Info

Product

Resources

About