Membrane filter-assisted surface enhanced Raman spectroscopy for the rapid detection of E. coli O157:H7 in ground beef

Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

“…Singleplex and multiplex detection of E. coli, S. aureus, Campylobacter, Listeria, and Bacillus anthracis spores coupled with immunomagnetic separation (IMS), microfluidics, and filtration have been reported (19,43,77,104,121,134). Detection has been evaluated in some food matrices, including ground beef, peanut butter, apple juice, and orange juice (19,43,77,121). Representative LODs were 15 to 35 CFU/ml in pure culture (134) and 10 2 CFU/ml in apple juice (77) without enrichment and 10 CFU/ml in ground beef after 2 h of enrichment (19).…”

“…Detection has been evaluated in some food matrices, including ground beef, peanut butter, apple juice, and orange juice (19,43,77,121). Representative LODs were 15 to 35 CFU/ml in pure culture (134) and 10 2 CFU/ml in apple juice (77) without enrichment and 10 CFU/ml in ground beef after 2 h of enrichment (19). Such low LODs in real food samples, even with a short enrichment period, are exceptional.…”

Pathogen enrichment device (PED) enables one-step growth, enrichment and separation of pathogen from food matrices for detection using bioanalytical platforms

“…Biosensor platforms have been used for detection of foodborne pathogens, including fiber optic, surface plasmon resonance (SPR), Raman, Fourier transformed infrared (FTIR) spectroscopy, flow cytometry, and impedance-based microfluidic devices (Bae and Bhunia, 2013;Bhunia, 2014;Bisha and Brehm-Stecher, 2009;Cho et al, 2015a;Najafi et al, 2014;Sharma and Mutharasan, 2013;Velusamy et al, 2010). Here we employed a forward light scattering sensor, BARDOT (bacterial rapid detection using optical scattering technology) for detection of colonies of target pathogens.…”

Precision Target Guide Strategy for Applying SERS into Environmental Monitoring

“…The combination of SERS-active structures with magnetic materials is an effective method to endow the substrate with facile recycle property, many different magnetic structures were applied into the SERS detection of pollutant, the improved ability of recycle and separation benefits a lot to the fast and convenient observation [17,48,58,59]. In addition to the magnetic field-assisted separation strategy, some other techniques were also performed for effectively separating the substrates captured with targets form the matrix, by using such as a membrane filter, [60] filter paper [61] and even commercial tap-based substrates [61]. Besides the mentioned traditional methods for sample concentration and separation, there are several other typical strategies that have been applied effectively, such as electrochemical concentration, hydrophobic concentration, paper-based substrate for separation and hydrogel-based structure for target gathering.…”