Pathogen enrichment device (PED) enables one-step growth, enrichment and separation of pathogen from food matrices for detection using bioanalytical platforms

Hahm, Byoung-Kwon; Kim, Hyochin; Singh, Atul K.; Bhunia, Arun K.

doi:10.1016/j.mimet.2015.07.016

Cited by 23 publications

(9 citation statements)

References 66 publications

(74 reference statements)

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“…Moreover, a brief culturing (enrichment) is recommended for all commercially available rapid methods. This is because culturing increases target microorganism numbers, thus essential for addressing ‘zero tolerance’ policy for many food‐borne pathogens, where a single viable cell per 25 g of analytical unit can be detected with high accuracy (Swaminathan and Feng, ; Hahm et al ., ). BARDOT fits well with the culturing method and can be used as a screening tool to isolate suspect colonies for further verification by polymerase chain reaction, next‐generation sequencing and mass‐spectrometry (Bhunia, ).…”

Section: Resultsmentioning

confidence: 97%

“…Recently, we also applied BARDOT to study the streptomycin‐induced stress response in Salmonella enterica serovar Typhimurium based on differential colony scatter patterns (Singh et al ., ). In addition, it was also used as a bioanalytical detection tool to validate performance of a sample processing and enrichment device, a pathogen enrichment device (Hahm et al ., ).…”

Section: Introductionmentioning

confidence: 97%

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Optical scatter patterns facilitate rapid differentiation of Enterobacteriaceae on CHROMagar^TM Orientation medium

Singh

Bhunia

2015

Microbial Biotechnology

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Summary E nterobacteriaceae family comprised pathogens and commensals and has a significant impact on food safety and public health. E nterobacteriaceae is often enumerated and presumptively identified on chromogenic media, such as CHROMagarTM Orientation medium based on colony profile; however, classification is highly arbitrary, and some could not be differentiated due to similar chromogen production. Here, we investigated the ability of the laser optical sensor, BARDOT (bacterial rapid detection using optical scattering technology) for rapid screening and differentiation of colonies of the major bacterial genera from E nterobacteriaceae on CHROMagarTM Orientation. A total of 36 strains representing 12 genera and 15 species were used to generate colony scatter image library that comprised 1683 scatter images. This library was used to differentiate mixed cultures of E nterobacteriaceae family – K lebsiella pneumoniae, E nterobacter spp., C itrobacter freundii and S erratia marcescens (KECS group); P roteus mirabilis, M organella morganii and P rovidencia rettgeri (PMP group); and non‐E nterobacteriaceae family: P seudomonas aeruginosa, A cinetobacter spp. and S taphylococcus aureus (PAS group) – and data show high accuracy (83–100%) for intra‐group classification of colonies in 10–22 h or even before visible production of chromogens. BARDOT successfully differentiated the major genera, including the ones that do not produce visually distinguishable chromogens on CHROMagarTM Orientation, providing a label‐free, real‐time on‐plate colony screening tool for E nterobacteriaceae.

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Section: Resultsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Optical scatter patterns facilitate rapid differentiation of Enterobacteriaceae on CHROMagar^TM Orientation medium

Singh

Bhunia

2015

Microbial Biotechnology

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“…coli O157:H7, Salmonella enterica, and L . monocytogenes , respectively could be detected via quantitative PCR (qPCR) after an enrichment period of 7 h [60]. This demonstrated that enrichment is a helpful step to remove inhibitors, improve the recovery of pathogens, and enhance the reaction stability for detection.…”

Section: Discussionmentioning

confidence: 99%

A Dual Filtration-Based Multiplex PCR Method for Simultaneous Detection of Viable Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus on Fresh-Cut Cantaloupe

Feng

Jiang

et al. 2016

PLoS ONE

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Fresh-cut cantaloupe is particularly susceptible to contamination with pathogenic bacteria, such as Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus. Therefore, development of rapid, yet accurate detection techniques is necessary to ensure food safety. In this study, a multiplex PCR system and propidium monoazide (PMA) concentration were optimized to detect all viable pathogens in a single tube. A dual filtration system utilized a filtration membrane with different pore sizes to enrich pathogens found on fresh-cut cantaloupe. The results revealed that an optimized multiplex PCR system has the ability to effectively detect three pathogens in the same tube. The viable pathogens were simultaneously detected for PMA concentrations above 10 μg/ml. The combination of a nylon membrane (15 μm) and a micro pore filtration membrane (0.22 μm) formed the dual filtration system used to enrich pathogens. The achieved sensitivity of PMA-mPCR based on this dual filtration system was 2.6 × 103 cfu/g for L. monocytogenes, 4.3 × 10 cfu/g for E. coli O157:H7, and 3.1 × 102 cfu/g for S. aureus. Fresh-cut cantaloupe was inoculated with the three target pathogens using concentrations of 103, 102, 10, and 1 cfu/g. After 6-h of enrichment culture, assay sensitivity increased to 1 cfu/g for each of these pathogens. Thus, this technique represents an efficient and rapid detection tool for implementation on fresh-cut cantaloupe.

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“…Over the last several decades, there have been significant developments in rapid Salmonella detection methods using multiple novel approaches reducing preenrichment or detection time (e.g., In-situ immuno-gold nanoparticle network ELISA biosensors, immunomagnetic separation, automated microfiltration system, pathogen enrichment device, laser optical sensor, modified media, isothermal amplication) [79][80][81][82][83][84][85][86][87][88][89]. (Figure 2).…”

Section: Salmonella Detectionmentioning

confidence: 99%

Salmonella in Shell Eggs: Mechanisms, Prevention and Detection

Seockmo¹,

Eduardo²,

Thomas³

et al. 2016

J Nutr Food Sci

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Contaminated shell egg is one of the key Salmonella infection routes for human, causing food-borne illnesses. Multiple salmonellosis infections due to egg contamination still occur in developed countries even though preventive methods, such as vaccination or washing followed by rinsing process, are carried out following certified national standards. Recent outbreaks indicated that current strategies for Salmonella control need to be optimized to further minimize contamination of commercial eggs. Therefore, there is a critical need to develop more sensitive and rapid Salmonella detection methods. In this review, we address (i) egg production; (ii) preventive methods that minimize contamination; (iii) mechanisms of Salmonella contamination; (iv) Salmonella detection methods.

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Pathogen enrichment device (PED) enables one-step growth, enrichment and separation of pathogen from food matrices for detection using bioanalytical platforms

Cited by 23 publications

References 66 publications

Optical scatter patterns facilitate rapid differentiation of Enterobacteriaceae on CHROMagar^TM Orientation medium

Optical scatter patterns facilitate rapid differentiation of Enterobacteriaceae on CHROMagar^TM Orientation medium

A Dual Filtration-Based Multiplex PCR Method for Simultaneous Detection of Viable Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus on Fresh-Cut Cantaloupe

Salmonella in Shell Eggs: Mechanisms, Prevention and Detection

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Pathogen enrichment device (PED) enables one-step growth, enrichment and separation of pathogen from food matrices for detection using bioanalytical platforms

Cited by 23 publications

References 66 publications

Optical scatter patterns facilitate rapid differentiation of Enterobacteriaceae on CHROMagarTM Orientation medium

Optical scatter patterns facilitate rapid differentiation of Enterobacteriaceae on CHROMagarTM Orientation medium

A Dual Filtration-Based Multiplex PCR Method for Simultaneous Detection of Viable Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus on Fresh-Cut Cantaloupe

Salmonella in Shell Eggs: Mechanisms, Prevention and Detection

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Optical scatter patterns facilitate rapid differentiation of Enterobacteriaceae on CHROMagar^TM Orientation medium

Optical scatter patterns facilitate rapid differentiation of Enterobacteriaceae on CHROMagar^TM Orientation medium