Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Chen, Jing; Park, Bosoon

doi:10.4315/0362-028x.jfp-15-516

Cited by 34 publications

(13 citation statements)

References 128 publications

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“…The immune-based detection methodologies exploit the affinity of antibodies for specific target antigens found on the surface of the desired microorganism. The use of nucleic acid-based technologies recognizes unique and highly specific DNA or RNA sequences that can either be sequenced, amplified and visualized on a gel, or otherwise differentiated for detection, quantification, and molecular typing (Yolken, 1988; Manfreda and De Cesare, 2005; Maciorowski et al., 2006; Mandal et al., 2011; Gharst et al., 2013; Park et al., 2014; Välimaa et al., 2015; Baker et al., 2016; Chen and Park, 2016; Zeng et al., 2016). Both immune and nucleic acid-based approaches are summarized in Table 1 (Immune) and Table 2 (Nucleic-acid).…”

Section: Rapid Detection Of Campylobacter—general Conceptsmentioning

confidence: 99%

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

et al. 2019

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The accurate and rapid detection of Campylobacter spp. is critical for optimal surveillance throughout poultry processing in the United States. The further development of highly specific and sensitive assays to detect Campylobacter in poultry matrices has tremendous utility and potential for aiding the reduction of foodborne illness. The introduction and development of molecular methods such as polymerase chain reaction (PCR) have enhanced the diagnostic capabilities of the food industry to identify the presence of foodborne pathogens throughout poultry production. Further innovations in various methodologies, such as immune-based typing and detection as well as high throughput analyses, will provide important epidemiological data such as the identification of unique or region-specific Campylobacter. Comparable to traditional microbiology and enrichment techniques, molecular techniques/methods have the potential to have improved sensitivity and specificity, as well as speed of data acquisition. This review will focus on the development and application of rapid molecular methods for identifying and quantifying Campylobacter in U.S. poultry and the emergence of novel methods that are faster and more precise than traditional microbiological techniques.

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Section: Rapid Detection Of Campylobacter—general Conceptsmentioning

confidence: 99%

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

et al. 2019

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“…These images are illustrated in the results section. [2,14] Construction of membrane with MIP modifier Both graphite and gold were applied to construct the body of the microelectrode. However, because of the price of gold, a graphite microelectrode was used in this study.…”

Section: Sensor Simulationmentioning

confidence: 99%

“…While the most important factor for the food industry is food safety, this concern has been resolved, to a certain extent, through the application of biosensors. [2] The convergence of nanotechnology, in accordance with its many capabilities, and food science has led to more than 200 large worldwide companies investing in new products in the field of nanotechnology. Based on the tremendous potential for nanotechnology applications in the food industry, it is expected that this technology will initiate a major revolution in food and agricultural products that will have positive implications for the worldwide expansion of mechanized agriculture.…”

Section: Introductionmentioning

confidence: 99%

Staphylococcus aureus exotoxin detection using potentiometric nanobiosensor for microbial electrode approach with the effects of pH and temperature

Ahari

Hedayati

Akbari‐adergani

et al. 2017

International Journal of Food Properties

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Considering the ever-increasing population and trends in industrialization, it is difficult to detect the toxins produced in food products using traditional techniques. In this study, a potentiometric nanobiosensor technique using selective patterns for Staphylococcus aureus exotoxin was thoroughly considered. A molecular framework and polymer were produced using methacrylic acid (MAA) monomers, which formed covalent bonds between MAA monomers to produce a white polymer. In addition, hydrogen bonds formed between the amino acids of the exotoxin and the MAA functional groups, which functioned as selective sites for the polymer. To evaluate the effect of pH on the S. aureus exotoxin nanobiosensor, diluted solutions of NaOH and HNO 3 were applied for the upper and lower pH levels, respectively. The effect of temperature was tested using distilled water at fixed temperatures. The results showed that the molecular framework polymer (MFP) in the designed biosensor was able to detect an exotoxin density up to 10-3 M at 68 nm of synthesized molecularly imprinted polymer (MIP) during the first 32 days of the experiment (from a total of 56 days). The potential differences remained constants at an optimum pH range of 5.0-8.5 and at an optimum temperature range of 15°C-25°C. Therefore, we concluded that the pH and temperature can affect the precision of a potentiometric nanobiosensor for detecting S. aureus exotoxin.

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“…Quantum dots possess exceptional photophysical properties, such as dimensionally tuned narrow and symmetric emission, broad and strong quantum yield, and high stability to photochemical and chemical radiation, and thus occupy a new class in the group of inorganic fluorescent labeling tools [23,[25][26][27]. In addition to their optical properties, CdSe/ZnS QDs, which consist of a CdSe core and a ZnS sheath that increase the QDs' resistance to light and increase their quantum yield, are the most popular among scientists [28,29]. The use of such QDs is widespread in the life sciences, but several publications have reported the use of QDs in the food sciences, where they were mostly used to detect pathogenic bacteria, proteins, and rotaviruses.…”

Section: Introductionmentioning

confidence: 99%

“…The use of such QDs is widespread in the life sciences, but several publications have reported the use of QDs in the food sciences, where they were mostly used to detect pathogenic bacteria, proteins, and rotaviruses. They were also used in applying polyclonal antibody-bound QDs and to intensify the signal of detecting allergens [23,[25][26][27][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Use of IHF-QD Microscopic Analysis for the Detection of Food Allergenic Components: Peanuts and Wheat Protein

Kalčáková

Tremlová

Pospiech

et al. 2020

Foods

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The aim of the study was to analytically evaluate quantum dots in immunohistofluorescence (IHF-QD) microscopic imaging as detectors of food allergens—peanut and wheat. The experiment was designed as two in silico experiments or simulations: (a) models of pastry samples were prepared with the addition of allergenic components (peanut and wheat protein components) and without the addition of allergenic components, and (b) positive and negative commercial samples underwent food allergen detection. The samples from both simulations were tested by the ELISA and IHF-QD microscopic methods. The primary antibodies (secondary antibodies to a rabbit Fc fragment with labeled CdSe/ZnS QD) were labelled at 525, 585, and 655 nm emissions. The use of quantum dots (QDs) has expanded to many science areas and they are also finding use in food allergen detection, as shown in the study. The study indicated that differences between the ELISA and IHF-QD microscopic methods were not observable among experimentally produced pastry samples with and without allergenic components, although differences were observed among commercial samples. The important value of the study is certainly the differences found in the application of different QD conjugates (525, 585, and 655). The highest contrast was found in the application of 585 QD conjugates that can serve for the possible quantification of present food allergens—peanuts and wheat. The study clearly emphasized that QD can be used for the qualitative detection of food allergens and can represent a reliable analytical method for food allergen detection in different food matrixes.

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Recent Advancements in Nanobioassays and Nanobiosensors for Foodborne Pathogenic Bacteria Detection

Cited by 34 publications

References 128 publications

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

Staphylococcus aureus exotoxin detection using potentiometric nanobiosensor for microbial electrode approach with the effects of pH and temperature

Use of IHF-QD Microscopic Analysis for the Detection of Food Allergenic Components: Peanuts and Wheat Protein

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