Developments in Rapid Detection Methods for the Detection of Foodborne Campylobacter in the United States

Abstract

The accurate and rapid detection of Campylobacter spp. is critical for optimal surveillance throughout poultry processing in the United States. The further development of highly specific and sensitive assays to detect Campylobacter in poultry matrices has tremendous utility and potential for aiding the reduction of foodborne illness. The introduction and development of molecular methods such as polymerase chain reaction (PCR) have enhanced the diagnostic capabilities of the food industry to identify the presence of foodborne pathogens throughout poultry production. Further innovations in various methodologies, such as immune-based typing and detection as well as high throughput analyses, will provide important epidemiological data such as the identification of unique or region-specific Campylobacter. Comparable to traditional microbiology and enrichment techniques, molecular techniques/methods have the potential to have improved sensitivity and specificity, as well as speed of data acquisition. This review will focus on the development and application of rapid molecular methods for identifying and quantifying Campylobacter in U.S. poultry and the emergence of novel methods that are faster and more precise than traditional microbiological techniques.

Co-occurrence of Campylobacter Species in Children From Eastern Ethiopia, and Their Association With Environmental Enteric Dysfunction, Diarrhea, and Host Microbiome

“…The fastidious nature of Campylobacter and difficulty of isolation, rendered culture and isolation unreliable for estimation of prevalence (32). By consequence, other studies utilized different approaches to estimate the prevalence of Campylobacter in stool samples, including enzyme immunoassay (EIA), PCR, and shotgun metagenomics (7,33,34). Two different culture independent approaches (conventional PCR and MeTRS) were used in this study to estimate the prevalence of Campylobacter in the child stool samples.…”

The use of photography to perform an online consumer test on the freshness of chicken breast and the extension of shelf life

“…The loss of adequacy might occur due to microbial, chemical, and/or physical modifications that result in detectable sensory traits recognized as spoilage and/or loss of the safety status of the product (Vorst et al, ). Chicken breast might be contaminated with pathogens (Pulido‐Landínez, ; Ricke et al, ); however, as it is not a ready‐to‐eat product, microbial safety is not a major concern when defining its shelf life (Pereira, Silva, Matos, & Patarata, ). Moreover, it has been demonstrated that pathogens do not compete well with the spoilage microbiota of fresh meat during storage (EFSA BIOHAZ Panel, ).…”

Highly Sensitive Detection ofCampylobacter spp.in Chicken Meat using a Silica Nanoparticle Enhanced Dot Blot DNA Biosensor

“…Culture-independent molecular methods, such as PCR, real-time PCR and qPCR, are used as alternatives to colony growth (De Boer et al 2015;Fontanot et al 2014;Gosselin-Théberge et al 2016;Liu et al 2017;Ricke et al 2019). However, PCR-based methods may also provide false-negative responses because of the sensitivity of DNA polymerase to inhibitors present in food matrices and enrichment broths (Schrader et al 2012;Vidic et al 2019).…”