Melatonin receptor 1A gene RsaI and inhibin alpha subunit gene HaeII polymorphisms in Honamli and Hair goat breeds reared in Western Mediterranean region of Turkey

Ağaoğlu, Özgecan Korkmaz; Saatcı, Mustafa; Akyüz, Bilal; Elmaz, Özkan; Çolak, Mehmet; Balkan, Bedih; Zeytünlü, Emel

doi:10.3906/vet-1409-31

Cited by 7 publications

(10 citation statements)

References 22 publications

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“…In Peranakan Etawa population, GG, AG and AA genotypes frequencies were 84.87%, 13.45% and 1.68%, respectively ( Table 1). The results of Peranakan Etawa are similar to those previously reported by Agaoglu et al (2015) which identifies the genetic diversity of the INHA genes in the Honamli and Hair goat populations in Turkey. Agaoglu et al (2015) report that GG and AG are common genotypes, whereas AA genotype and A allele are found in relatively small frequencies.…”

Section: Resultssupporting

confidence: 89%

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Genetics Polymorphisms of INHA in Local Goat Populations in South-West Sulawesi Region of Indonesia

Dagong¹,

Bugiwati²,

Rahim³

et al. 2020

American Journal of Animal and Veterinary Sciences

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Inhibin subunit alpha (INHA) is one important gene that control reproductive characteristics of animal. The aim of this research was to identify the genetic diversity of INHA gene of Kacang and Peranakan Etawa (PE) goat as local goat breed which were maintained by farmers at goat development centers in Sulawesi region, Indonesia. DNA samples were collected from 137 heads Kacang breed from Jeneponto, South Sulawesi and 119 heads of Peranakan Etawa (PE) from Polewali Mandar regency, West Sulawesi. The PCR-RFLP technique with HaeII restriction enzyme was used to identify the genetic diversity base on the INHA gene. A and G alleles and AA, AG and GG genotypes of INHA gene were could identified in the local goat populations. Frequency of G allele, as predominant allele, were found in both populations (0.9343 of Kacang and 0.9160 of Peranakan Etawa goats). Both of local goat populations were shown low genetic diversity with average observed heterozygosity (H o) of INHA gene were 0.13. Nevertheless, the data obtained in this study can be used as preliminary information to determine some development and improvement strategies of genetic quality of local goats that exist in this region.

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Section: Resultssupporting

confidence: 89%

“…INHA gene fragment identified by using PCR-RFLP method. Fragments of INHA gene was amplified with forward and reverse primer sequences as follow F: 5'-CCACACAGGACTGGACAGACA-3' and R: 5'-GCAGGAACAGAGAGGACAACG-3' (Agaoglu et al, 2015) with a predicted 217 bp length of amplicon product.…”

Section: Pcr Amplification and Genotyping Of Inha Genementioning

confidence: 99%

Genetics Polymorphisms of INHA in Local Goat Populations in South-West Sulawesi Region of Indonesia

Dagong¹,

Bugiwati²,

Rahim³

et al. 2020

American Journal of Animal and Veterinary Sciences

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“…However, melatonin receptor 1A (MTNR1A) is the main receptor that mediates the reproductive and circadian actions of melatonin in sheep. Polymorphisms in the MTNR1A gene have been documented to be associated with seasonal reproduction in various breeds of sheep (Carcangiu et al, 2009a;Ahmad et al, 2015), goat (Agaoglu et al, 2015), pig (Ramírez et al, 2009), deer (Yang et al, 2014) and buffalo (Barbosa et al, 2017). Arylalkylamine N-acetyltransferase (AA-NAT) is called "the Timezyme" because it plays a unique role in vertebrate timekeeping (Klein, 2007) and is considered the rate-limiting enzyme in melatonin biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

Genetic polymorphism in melatonin receptor 1A and arylalkylamine N-acetyltransferase and its impact on seasonal reproduction in Egyptian sheep breeds

Fathy¹,

Gouda

Gafer³

et al. 2018

Arch. Anim. Breed.

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This study was carried out to detect polymorphisms in the melatonin receptor 1A (MTNR1A) and arylalkylamine N-acetyltransferase (AA-NAT) genes and their association with reproductive traits. Blood samples of 126 animals from three Egyptian sheep breeds were collected. DNA was extracted and subjected to PCR restriction fragment length polymorphism (RFLP) analysis using the RsaI and SmaI enzymes. Two alleles (C and T) and three genotypes (CC, CT and TT) for MTNR1A and for AA-NAT (A and G; GG, GA and AA) were detected. The alleles C and A and the genotypes CT and GA showed the highest frequencies for the MTNR1A and AA-NAT genes, respectively. Association analysis of the MTNR1A single nucleotide polymorphism (SNP) with ewe reproductive traits revealed significant associations in the Ossimi and Rahmani breeds with age at first lambing, and the C allele seemed to be the favorable allele. The results for the AA-NAT SNP demonstrated significant correlations in Ossimi with age at first lambing and litter size and in Rahmani with lambing interval; the G allele seemed to be the desirable allele. In the first conception season, ewes carrying CT exhibited a significantly lower age of first lambing in the unfavorable season. Additionally, GG ewes exhibited a significantly lower age of first lambing in the early favorable season, followed by the unfavorable season. To the best of our knowledge, this is the first study of these associations in Egyptian sheep breeds. In conclusion, the polymorphisms revealed in this study could be used as genetic markers to improve reproductive efficiency during the unfavorable season, and the obtained desirable genotypes could be considered in new genetic selection schemes.

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“…The first investigation on the goat MTNR1A polymorphism was conducted in 2002 and evidenced the existence of seven MnlI sites, yet none showed polymorphism [46]. Since this study, others have been conducted on different goat breeds [38,[47][48][49][50][51]. In general, no polymorphisms have been identified by the MnlI restriction enzyme, with all the goat breeds investigated showing an M/M genotype.…”

Section: Introductionmentioning

confidence: 83%

“…For RsaI enzyme, only two genotypes have been identified to date: R/R and R/r. Specifically, the R/R genotype is the most common, whereas the R/r genotype was found in only a limited number of studies and within a small proportion of the investigated goat breed populations [38,[46][47][48][49][50][51]. Regarding the relationship between gene polymorphism and reproductive activity, studies remain scarce and contradictory.…”

Section: Introductionmentioning

confidence: 99%

Polymorphism of the MTNR1A Melatonin Receptor Gene in Goat Breeds of Northern Italy

Agradi,

Luridiana,

Mura

et al. 2023

Animals

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Melatonin receptor MT1, encoded by the MTNR1A gene, is the main one involved in the seasonal regulation of reproductive activity. A correlation between this gene polymorphism and reproductive performance has been demonstrated in sheep. To date, no data about MTNR1A gene polymorphism are available regarding Italian goat breeds other than the Sarda goat. This study aimed to detect any PCR-RFLP polymorphic sites of MTNR1A using MnlI and RsaI enzymes in Northern Italian goat breeds, which are characterized by a pronounced reproductive seasonality. One-hundred-eight adult female goats belonging to four different breeds were included in the study (i.e., Frisa Valtellinese, n = 29; Orobica, n = 23; Lariana, n = 29; Camosciata delle Alpi, n = 27). Blood was sampled from each goat. Genomic DNA was extracted from each sample and the main part of exon II of MTNR1A gene was amplified by PCR and digested with MnlI and RsaI enzymes. Unexpectedly, none of the fragments were found to be polymorphic. The absence of polymorphism may be linked to the macro group of goat breeds that evolved during human migrations. Breeds of the Alpine–European strain would appear to show no polymorphism, as confirmed by our study, whereas breeds belonging to the Mediterranean–African or Asian–Middle Eastern strains do.

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Melatonin receptor 1A gene RsaI and inhibin alpha subunit gene HaeII polymorphisms in Honamli and Hair goat breeds reared in Western Mediterranean region of Turkey

Cited by 7 publications

References 22 publications

Genetics Polymorphisms of INHA in Local Goat Populations in South-West Sulawesi Region of Indonesia

Genetics Polymorphisms of INHA in Local Goat Populations in South-West Sulawesi Region of Indonesia

Genetic polymorphism in melatonin receptor 1A and arylalkylamine N-acetyltransferase and its impact on seasonal reproduction in Egyptian sheep breeds

Polymorphism of the MTNR1A Melatonin Receptor Gene in Goat Breeds of Northern Italy

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