MEKC combined with SPE and sample stacking for multiple analysis of pesticides in water samples at the ng/L level

Ravelo‐Pérez, Lidia M.; Hernández-Borges, Javier; Cifuentes, Alejandro; Rodríguez‐Delgado, Miguel Ángel

doi:10.1002/elps.200600526

Cited by 29 publications

(25 citation statements)

References 31 publications

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“…Analytes are dissolved in a 1:3 solution made of water/100 mM sodium tetraborate at pH 8.5 v/ v. A high voltage (222 kV) is then applied and the electric current monitored to control sample matrix removal from the capillary. When the current becomes 95-99% of the value obtained with the BGE, the voltage is turned off and the polarity reversed to run the separation [21].…”

Section: Conditions For Repsmmentioning

confidence: 99%

“…This group of pesticides has been previously separated by our team [21] by MEKC and determined in different waters samples using a SPE procedure for off-line preconcentration and reversed-electrode polarity stacking mode (REPSM) as on-line preconcentration strategy. REPSM provided a high sensitivity improvement (LODs between 33.6 and 216 mg/L) with respect to nonstacking conditions (few mg/L).…”

Section: Introductionmentioning

confidence: 99%

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Multiple pesticide analysis in wine by MEKC combined with solid‐phase microextraction and sample stacking

et al. 2007

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In this work, a new method for the determination in white wines of 12 pesticides widely used in vine cultivars (namely, carbendazim, pirimicarb, metalaxyl, pyrimethanil, procymidone, nuarimol, azoxystrobin, tebufenozide, fenarimol, benalaxyl, penconazole, and tetradifon) using solid-phase microextraction (SPME) and MEKC with diode-array detection (DAD) was developed. The MEKC buffer consisted of 100 mM sodium tetraborate and 30 mM SDS at pH 8.5 with 6% v/v 1-propanol. Reversed-electrode polarity stacking mode (REPSM) was applied as on-line preconcentration strategy. In order to carry out an effective and sensitive determination of these pesticides in wine samples, an off-line SPME procedure was optimized by means of an experimental design. After studying the extraction performance of different SPME coatings, PDMS/divinylbenzene (PDMS/DVB) fibers were found the most appropriate for the extraction of most of these pesticides. Carbendazim and metalaxyl could not be extracted from wine samples. Calibration curves for extracted standards and fortified white wines were studied in order to determine the presence of a matrix effect. The combination of both preconcentration procedures (SPME and REPSM) allowed the determination of ten of these pesticides in white wines at concentrations between 0.054 and 0.113 mg/L. (i.e., levels well below the maximum residue limits (MRLs) allowed for these compounds in wine grapes). Ten homemade wines were they analyzed with the optimized method demonstrating the usefulness of the proposed procedure.

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Section: Conditions For Repsmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Multiple pesticide analysis in wine by MEKC combined with solid‐phase microextraction and sample stacking

et al. 2007

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“…Most recently, Ravelo-Pérez et al [86] achieved improved separations of pesticides. Authors demonstrated that the combined use of both, on-column and off-column pre-concentration techniques with MEKC-UV was beneficial to achieve sensitive and simultaneous detection of twelve pesticides, namely, carbendazinm, pirimicarb, metalaxyl, pyrimethanil, procymidone, nuarimol, azoxystrobin, tebufenozide, fenarimol, benalaxyl, penconazol, and tetradifon ( Figure 3).…”

Section: Toxins Contaminants Pesticides and Residuesmentioning

confidence: 99%

Recent advances in the application of capillary electromigration methods for food analysis

Cifuentes¹

2006

Electrophoresis

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“…Recently, RaveloPerez et al [72] proposed the development of a new analytical strategy that combines MEKC-UV analysis with SPE as an off-line preconcentration technique, and reversedelectrode polarity stacking mode (REPSM) as on-line stacking procedure for the simultaneous separation and ultrasensitive determination of 12 pesticides in mineral, stagnant and tap waters. However, GC and HPLC continue being the suggested techniques by official international organizations.…”

Section: Ce)mentioning

confidence: 99%

Thirty years of capillary electrophoresis in food analysis laboratories: Potential applications

2011

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CE has generated considerable interest in the research community since instruments were introduced by different trading companies in the 1990s. Nowadays, CE is popular due to its simplicity, speed, highly efficient separations and minimal solvent and reagent consumption; it can also be included as a useful technique in the nanotechnology field and it covers a wide range of specific applications in different fields (chemical, pharmaceutical, genetic, clinical, food and environmental). CE has been very well evaluated in research laboratories for several years, and different new approaches to improve sensitivity (one of the main drawbacks of CE) and robustness have been proposed. However, this technique is still not well accepted in routine laboratories for food analysis. Researching in data bases, it is easy to find several electrophoretic methods to determine different groups of analytes and sometimes they are compared in terms of sensitivity, selectivity, precision and applicability with other separation techniques. Although these papers frequently prove the potential of this methodology in spiked samples, it is not common to find a discussion of the well-known complexity of the matrices to extract analytes from the sample and/or to study the interferences in the target analytes. Summarizing, the majority of CE scientific papers focus primarily on the effects upon the separation of the analytes while ignoring their behavior if these analytes are presented in real samples.

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MEKC combined with SPE and sample stacking for multiple analysis of pesticides in water samples at the ng/L level

Cited by 29 publications

References 31 publications

Multiple pesticide analysis in wine by MEKC combined with solid‐phase microextraction and sample stacking

Multiple pesticide analysis in wine by MEKC combined with solid‐phase microextraction and sample stacking

Recent advances in the application of capillary electromigration methods for food analysis

Thirty years of capillary electrophoresis in food analysis laboratories: Potential applications

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