Recent advances in the application of capillary electromigration methods for food analysis

Cifuentes, Alejandro

doi:10.1002/elps.200500474

Cited by 150 publications

(96 citation statements)

References 277 publications

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“…All results including LOD, linear ranges and RSD for fluorescent dyes, carbohydrates and L-alanine were listed in Table 1. Its performance in RBI detection was better than those mentioned in the former report [38], while its fluorescent sensitivity was comparable to the achievable sensitivity by conventional LIF systems [28]. Even if merely considering its accomplishment to compromise high sensitivity and universal analysis, the reported CE detection scheme is of certain significance.…”

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confidence: 74%

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Simultaneous LIF and retro‐reflected beam interference detection for CE

et al. 2008

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This Short Communication describes a novel optical detection method for CE, based on the combination of LIF detection and retro-reflected beam interference detection. By the use of a side-illuminated laser beam, an on-column multifunctional detection for CE has been developed, and some key elements in the scheme have been optimized. In addition, two miscellaneous samples including fluorescent dyes, carbohydrates and amino acids have been determined to evaluate its performance. Without any additional pretreatment, sufficient LOD and linear ranges have been achieved for most analytes. Its performance in retro-reflected beam interference detection is better than those mentioned in a former report, and its fluorescent sensitivity is comparable with the achievable sensitivity by conventional LIF systems, all of which together combine high sensitivity and universal analysis to a certain extent.

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confidence: 74%

“…Unfortunately, most analytes need to be derived for their non-fluorescent property under conventional LIF detection [28,29]. For RI detection, being of universal and quantitative analysis, its comprehensive application is still far away because of its unsatisfactory sensitivity and complicated optical train [30][31][32][33][34][35].…”

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confidence: 99%

Simultaneous LIF and retro‐reflected beam interference detection for CE

et al. 2008

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“…Derivatization is a modification of analytes to give suitable structural features to increase the detection sensitivity and to make the inclusion and interaction of the derivatized amino acids with CDs possible [10,11]. Derivatization procedures by CE can be classified in three different modes based on the analytical stage where derivatization takes place: before (pre-capillary), during (in-capillary), or after (post-capillary) the electrophoretic separation.…”

Section: Introductionmentioning

confidence: 99%

Development of an in‐capillary derivatization method by CE for the determination of chiral amino acids in dietary supplements and wines

et al. 2009

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A fast in-capillary derivatization method by CE with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate was developed for the first time for the determination of amino acid enantiomers (arginine, lysine, and ornithine) in dietary supplements and wines. Because of the initial current problems due to the formation of precipitates into the capillary during the derivatization reaction, a washing step with an organic solvent as DMSO between injections was necessary. Different approaches were also investigated to enhance the sensitivity of detection. A derivatization procedure, where plugs of ACN, derivatizing agent (10 mM 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate), and sample in borate (1:1 v/v) were injected in tandem (2, 3, and 6 s, respectively, at 50 mbar), was selected because it enabled to obtain the most sensitive and reproducible results. Appropriate analytical characteristics (linearity, LOD and LOQ, precision, absence of matrix interferences, and accuracy) were obtained for this method. Finally, the optimized method was successfully applied to the determination of the enantiomers of arginine, lysine, and ornithine in food samples of different complexities (dietary supplements and wines).

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“…Among these, methods based on liquid chromatography 8,9 and enzymatic determination 10 are the most highly demanded, although methods based on Capillary Electrophoresis (CE) have also been proposed [11][12][13] . Several aspects make capillary electrophoresis a highly adequate technique to obtain simple and fast methods for glutathione determination, such as good reproducibility, simplicity of procedure, short analytical time, low injection volume, and low cost 14,15 . If we go through proposed methods based on capillary zone electrophoresis (CZE), a similarity is observed in the process of analyte separation, where the background analyte is usually constituted by borate in the 0.05-0.3 M concentration range, with pH values near its pK a 16,17 , BGE concentration and pH, together with capillary length and voltage applied having the strongest effect on the time required for analyte separation.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Glutathione and Glutathione Disulfide in an Acid Extract of Plant Shoot and Root by Capillary Electrophoresis

Mendoza

Garrido

Riveros

et al. 2008

J. Chil. Chem. Soc.

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This study describes the fast and simultaneous determination of glutathione and glutathione disulfide by Capillary Zone Electrophoresis in plant extracts of shoot and root of tomato plants. Frequent use of acidic precipitation of protein generates an acidic matrix of strength and pH that may cause changes in the method sensitivity, comigration of species or changes in the equilibria that relate both species in cells or fluids. In this study, the resulting acidic matrix was previously treated with the same background electrolyte to prevent comigration and to improve signal resolution. Optimization of some parameters of the technique allowed the determination of both analytes in less than three minutes. The optimized method showed good reproducibility and linearity, with correlation coefficients above 0.999 and detection limits below 3 µM for both peptides. Analyte recovery in the process was in the 88-104% range. The concentration found in tomato plants hydroponically grown in the absence of stress factors was in the 51-100 nmol g -1 range, fresh weight for GSH and 5-32 nmol g -1 range, fresh weight for GSSG.

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Recent advances in the application of capillary electromigration methods for food analysis

Cited by 150 publications

References 277 publications

Simultaneous LIF and retro‐reflected beam interference detection for CE

Simultaneous LIF and retro‐reflected beam interference detection for CE

Development of an in‐capillary derivatization method by CE for the determination of chiral amino acids in dietary supplements and wines

Simultaneous Determination of Glutathione and Glutathione Disulfide in an Acid Extract of Plant Shoot and Root by Capillary Electrophoresis

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