Media selection for mycelia growth, antifungal activity against wood-degrading fungi, and GC-MS study by Pycnoporus sanguineus

Abstract

Wood-decaying fungi present a serious threat to items made from rubberwood (Hevea brasiliensis). Though conventional chemical control has been a successful method for preserving wood against stain and decay fungi growth, the effects of these chemicals are of concern because they create problems for the environment and public health. Pycnoporus sanguineus (P. sanguineus), is a white-rot fungus that invades wood during its growth, storage, or use, causing decay or other property changes. It was considered in this work as a potential source of bioactive compounds and investigated for its natural antifungal activity using a minimum inhibitory concentration assay against wood-degrading fungi. It was found that media consisting of 10.0 g/L malt extract, yeast extract, dextrose, and maltose, respectively at pH 4.7±0.2 provided the highest biomass production by P. sanguineus. Results showed that the antifungal properties of methanol and water extract of P. sanguineus mycelia and supernatant ranged from MIC values of 0.1 to 5.0 µg/µL. 4H-Pyran-4-one,2,3-dihydro-3,5-dihydroxy-6-methyl- (DDMP) was found to be the major component in the extract of this fungus, based on analysis using gas chromatography – mass spectrometry.

Calligonum polygonoides L. Shrubs Provide Species-Specific Facilitation for the Understory Plants in Coastal Ecosystem

“…In this regard, it was found that flavonoids secreted as plant exudates act as chemical attractants to Rhizobia [76]. Moreover, 4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl, which is determined as a major flavonoid compound in the present study, has been reported as an antifungal agent [77]. Indeed, root exudates act as chemical messengers to regulate the microbial chemotaxis and interactions via a diverse array of molecular networks [78].…”

Physicochemical properties, bioactive components, antioxidant and antimicrobial potentials of some selected honeys from different provinces of Turkey

“…4H-Pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl-(DDMP) is a fraction of flavonoid that was detected in almost all samples except for H3 and H8. It had been reported as antifungal (Teoh et al, 2011).…”

Process simulation-integrated optimization of lignocellulolytic enzyme production

“…(2011) conducted a study to investigate the optimum medium composition for mycelial growth and antifungal activity of P. sanguineus. In this work, they found out that 10 g/l ME, YE, dextrose and maltose supported the highest fungal growth [27]. In the presence of readily available reducing sugar, fungi produce high levels of Lac enzyme [40].…”

“…Table 1 shows the concentration ranges of the medium components used in this study. The components and concentrations were selected as an interpretation of the authors' of the present study based on previous optimization studies on P. sanguineus [27] , Pleurotus ostreatus [28], and Phlebia brevispora [29]. The experiments performed were early exploratory investigations to understand the effect of nitrogen sources on the enzyme production.…”