Process simulation-integrated optimization of lignocellulolytic enzyme production

Abstract

P. sanguineus growth conditions are optimized for Lac, Bet, and Cmc activities.• A novel workflow integrating experiments and process simulations is offered.• Kriging is used to model time-profile data from activity measurements.• The workflow is demonstrated on a bioethanol production process case study.• Significance of Bet activity favors yeast extract dominated optimal growth medium.

Induction of cellulase production in Trichoderma reesei by a glucose–sophorose mixture as an inducer prepared using stevioside

“…1,2 In recent studies, Trichoderma reesei was the main producer used for commercial lignocellulolytic enzyme preparation; however, the high cost of inducers and low induction efficiency are the major bottlenecks limiting the biorening of lignocellulose. 3,4 Using inducers is essential for cellulase production. The inducers of T. reesei can be roughly divided into two categories: solid inducers and soluble inducers.…”

“…1,2 In recent studies, Trichoderma reesei was the main producer used for commercial lignocellulolytic enzyme preparation; however, the high cost of inducers and low induction efficiency are the major bottlenecks limiting the biorefining of lignocellulose. 3,4…”