Mechanistic evaluation of anti-arthritic activity of β-methylphenylalanine in experimental rats

Ren, Shi-Xiang; Zhang, Bo; Lin, Yuan; Ma, Desi; Li, Huan

doi:10.1016/j.biopha.2019.108730

Cited by 9 publications

(7 citation statements)

References 28 publications

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“…It is well known that CFA-evoked arthritis is similar to human arthritis clinical characteristics such as periarticular tissue, inflammation response, and oxidative stress. 19 In the present study, the intradermal administration of CFA resulted in paw edema, significant decreases in the nociceptive threshold, and high arthritis scores, indicating the rat’s arthritis was successfully established. Besides, we were first to demonstrate that SJF could relieve arthritis symptoms via inhibiting the inflammation response and oxidative stress in CFA-induced arthritis rats.…”

Section: Discussionsupporting

confidence: 54%

Styphnolobium japonicum (L.) Schott Flower Extract Alleviates Oxidative Stress and Inflammatory Factors in the Adjuvant-Induced Arthritis Rat Model

Liu¹,

Su²

2021

JPR

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Introduction This research was to evaluate the beneficial effects of Styphnolobium japonicum (L.) Schott flower extract (SJF) on the adjuvant-induced arthritis rat model. Methods Arthritis was evoked by injection of complete Freund’s adjuvant (CFA) in the hind paw. SJF (150 or 300 mg/kg/day) or Celecoxib (5 mg/kg/day) were administered intragastrically from the 0th day to the 28th day. The arthritis symptoms (paw edema, arthritic scores, mechanical hyperalgesia, and thermal hyperalgesia), inflammation biomarkers (RT and CRP), related enzymes (MMP1 and MMP13), oxidative stress markers (CAT, SOD, GPx, and MDA), and inflammatory cytokines (IL-6, IFN-γ, TNF-α, and IL-1β) of SJF-treated CFA rats were evaluated. Results CFA rats exhibited severe arthritis symptoms, increased oxidative stress, and inflammatory cytokines. Interestingly, SJF treatment relieving arthritis symptoms and restored body weight gain compared with those in the CFA group. SJF treatment decreased the levels of CRP, RF, MMP1, and MMP13 in the CFA group. Besides, SJF treatment increased the activities of antioxidant enzymes and decreased the MDA content and inflammatory cytokines compared with those in the CFA group. Moreover, SJF could increase the mRNA expression of GPx-1 and CAT and inhibit the mRNA expression of IL-6 and TNF-α in the ankle tissue of CFA rats.

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Section: Discussionsupporting

confidence: 54%

Styphnolobium japonicum (L.) Schott Flower Extract Alleviates Oxidative Stress and Inflammatory Factors in the Adjuvant-Induced Arthritis Rat Model

Liu¹,

Su²

2021

JPR

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“…Previous literatures have indicated that the increased serum levels of PGE 2 and MMP-3 attributed to inflammatory response and oxidative stress in RA (Bae et al 2003;Ren et al 2019b). An increased serum MMP-3 level is a sign of cartilage destruction in the early RA (Yamanaka et al 2000).…”

Section: Discussionmentioning

confidence: 96%

“…2003; Ren et al. 2019b). An increased serum MMP-3 level is a sign of cartilage destruction in the early RA (Yamanaka et al.…”

Section: Discussionmentioning

confidence: 99%

Coix lachryma-jobi extract ameliorates inflammation and oxidative stress in a complete Freund’s adjuvant-induced rheumatoid arthritis model

Zhang

Shi³

et al. 2019

Pharmaceutical Biology

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Context: Adlay seed [Job’s tears, Coix lachryma-jobi L. var. ma-yuen Stapf (Poaceae)] is a Traditional Chinese Medicine, which has been investigated to treat inflammatory diseases and rheumatism.Objective: This study evaluates the ameliorative effects of adlay seed extract (ASE) in a complete Freund’s adjuvant (CFA)-induced rheumatoid arthritis (RA) rats.Materials and methods: The RA Sprague–Dawley rat model was induced and randomly divided into six groups with or without ASE treatment (50, 100 or 200 mg/kg). After 28 d administration, the symptoms, biochemical parameters and molecular mechanisms were investigated.Results: The values of paw oedema, PGE2 and MMP-3 decreased from 1.46 ± 0.04 to 0.66 ± 0.07 cm3, from 126.2 ± 11.48 to 79.71 ± 6.8 pg/mL and from 142.7 ± 8.36 to 86.51 ± 5.95 ng/mL, respectively; the values of body weight increased from 177.25 ± 5.94 to 205 ± 6.52 g in HASE group. In addition, treatment of ASE reduced the levels of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6, MCP-1), and increased the activities of antioxidant enzyme (GSH-Px, SOD, and CAT). Furthermore, ASE could suppress the mRNA expression of COX-2 and CHI3L1 and improve the mRNA expression of CAT and GPx-1 in ankle tissues of RA rats.Discussion and conclusions: For the first time, our results indicated ASE exerts anti-RA effects via inhibiting pro-inflammatory factors and alleviating oxidative stress. Our finding sheds light on the research and development of anti-RA functional foods from adlay seed.

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“…Therefore, the levels of these antioxidant enzymes are crucial in determining the antioxidant activity of a therapeutic agent. FCA results in oxidative damage and increases stress in untreated arthritic rats/positive control (Ren et al., 2019). Secondary plant metabolites are helpful in the reduction of oxidative stress by improving antioxidant enzyme levels (Li et al., 2018).…”

Section: Discussionmentioning

confidence: 99%

Probing the antioxidant potential of Juglans regia (walnut) against arthritis‐induced oxidative stress in Sprague Dawley rats

Javed

Rakha

Butt

et al. 2022

Journal of Food Biochemistry

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Oxidative stress is the underlying cause of various chronic diseases and can contribute to its progression. Plant‐based therapeutics are effective in the prevention of various chronic diseases through the management of underlying causes. Walnuts owing to their rich phytochemistry possess antioxidative potential. In this context, the present research was designed to explore the therapeutic potential of walnut against arthritis‐induced oxidative stress in rat model. Purposely, 50 Sprague Dawley rats were separated into five groups of 10 rats each. The rats were categorized as G0 (negative control), G1 (positive control), G2 (methotrexate), G3 (walnut feed), and G4 (walnut extract). Walnut feed and extract significantly reduced oxidative stress by improving the antioxidant enzymes in arthritic rats. Total oxidative stress was reduced by 41.44% and 21.52% in G3 while 52.81% and 36.76% in G4 when compared with G1 and G2, respectively. Antioxidant enzyme levels in serum were significantly improved after the walnut‐based interventions. Evidently, superoxide dismutase level improved by 74.5% in G3 and 83.40% in G4, while catalase level increased by 51.99% in G3 and 61.34% in G4, respectively, when compared with G1. Liver function biomarkers, that is, ALP and AST, were decreased in G3 and G4 when compared with G1 and G2. The histopathological examination illustrated the promising role of walnut‐based interventions in conserving the structural integrity of hepatic and renal tissues. Meanwhile, gene expression analysis revealed that walnut treatments protected from oxidative damage by the downregulation of Dual‐oxidases expression. Conclusively, walnut feed and extract might serve as potent antioxidant intervention with no potential side effects. Practical applications The present work was aimed to evaluate the efficacy of walnut‐based interventions against arthritis‐induced oxidative stress. The walnut‐based interventions positively modulated the antioxidant enzymes, liver, and kidney functions, while downregulating the gene associated with oxidative stress in animal model. Consequently, the current findings suggest wider applications of walnuts to avoid free radical induced damage during arthritis; however, human intervention studies may be carried out to further understand the mechanism of their bioefficacy.

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Mechanistic evaluation of anti-arthritic activity of β-methylphenylalanine in experimental rats

Cited by 9 publications

References 28 publications

Styphnolobium japonicum (L.) Schott Flower Extract Alleviates Oxidative Stress and Inflammatory Factors in the Adjuvant-Induced Arthritis Rat Model

Styphnolobium japonicum (L.) Schott Flower Extract Alleviates Oxidative Stress and Inflammatory Factors in the Adjuvant-Induced Arthritis Rat Model

Coix lachryma-jobi extract ameliorates inflammation and oxidative stress in a complete Freund’s adjuvant-induced rheumatoid arthritis model

Probing the antioxidant potential of Juglans regia (walnut) against arthritis‐induced oxidative stress in Sprague Dawley rats

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