Coix lachryma-jobi extract ameliorates inflammation and oxidative stress in a complete Freund’s adjuvant-induced rheumatoid arthritis model

Abstract

Context: Adlay seed [Job’s tears, Coix lachryma-jobi L. var. ma-yuen Stapf (Poaceae)] is a Traditional Chinese Medicine, which has been investigated to treat inflammatory diseases and rheumatism.Objective: This study evaluates the ameliorative effects of adlay seed extract (ASE) in a complete Freund’s adjuvant (CFA)-induced rheumatoid arthritis (RA) rats.Materials and methods: The RA Sprague–Dawley rat model was induced and randomly divided into six groups with or without ASE treatment (50, 100 or 200 mg/kg). After 28 d administration, the symptoms, biochemical parameters and molecular mechanisms were investigated.Results: The values of paw oedema, PGE2 and MMP-3 decreased from 1.46 ± 0.04 to 0.66 ± 0.07 cm3, from 126.2 ± 11.48 to 79.71 ± 6.8 pg/mL and from 142.7 ± 8.36 to 86.51 ± 5.95 ng/mL, respectively; the values of body weight increased from 177.25 ± 5.94 to 205 ± 6.52 g in HASE group. In addition, treatment of ASE reduced the levels of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6, MCP-1), and increased the activities of antioxidant enzyme (GSH-Px, SOD, and CAT). Furthermore, ASE could suppress the mRNA expression of COX-2 and CHI3L1 and improve the mRNA expression of CAT and GPx-1 in ankle tissues of RA rats.Discussion and conclusions: For the first time, our results indicated ASE exerts anti-RA effects via inhibiting pro-inflammatory factors and alleviating oxidative stress. Our finding sheds light on the research and development of anti-RA functional foods from adlay seed.

Icariin enhances cell survival in lipopolysaccharide‑induced synoviocytes by suppressing ferroptosis via the Xc‑/GPX4 axis

“…2B), demonstrating that ICA could attenuate the iron deposit in LPS-induced synoviocytes. GPX, an antioxidant enzyme (35), was also detected in the present study. GPX levels were significantly reduced by LPS compared with the control group.…”

Chemical Composition of Pterospermum heterophyllum Root and its Anti-Arthritis Effect on Adjuvant-Induced Arthritis in Rats via Modulation of Inflammatory Responses

“…Although there are many anti-RA drugs in clinic, such as immunosuppressants, biological agents, DMARDs, steroidal drugs, and NSAIDs, most of them are associated with long-term adverse effects and costs (Li et al, 2019b;Dai et al, 2020;Zhu et al, 2020). In addition, the CFA-induced arthritis (AIA) model and the collagen-induced arthritis model are two typical preclinical experimental animal models of RA, and the former is a classic, easy-to-measure, short duration, reliable and reproducible test animal model, which is extensively used for the preclinical evaluation of anti-RA drugs since its pathological and morphological characteristics were similar to those of human RA Pan et al, 2017;Voon et al, 2017;Zhang et al, 2019;Saleem et al, 2020). The roots of P. heterophyllum have been widely used to treat RA as a vital TCM for centuries (Editorial Committee of Traditional Chinese Medicine 1999;, but their anti-RA effect and chemical profiling have not been reported so far.…”

Screening and Verification of Key Proteins in Patients with Rheumatoid Arthritis and Intervention of Traditional Chinese Medicine

“…Hs-CRP and ESR were commonly used in the laboratory to diagnose the activity of rheumatic diseases, and can be used as indicators to monitor the activity of diseases and evaluate the treatment stability. Association rules show that compared with single use Chinese traditional medicine group, XFC combined traditional Chinese medicine on IL-11, IL-17, PD-L2, hs-CRP, anti-CCP have obvious correlation, showed that after the combined traditional Chinese medicine can significantly improve the indicators, and the mechanism may be related to the pharmacological effects of XFC, semen coicis extract exerts anti-RA effects via inhibiting pro-inflammatory factors and alleviating oxidative stress [28];The active components of radix astragali can strengthened humoral and cellular immunity and improved the regulation of human immunity [29].…”