Mechanisms of seed ageing under different storage conditions for Vigna radiata (L.) Wilczek: lipid peroxidation, sugar hydrolysis, Maillard reactions and their relationship to glass state transition

Abstract

Two primary biochemical reactions in seed ageing (lipid peroxidation and non-enzymatic protein glycosylation with reducing sugars) have been studied under different seed water contents and storage temperatures, and the role of the glassy state in retarding biochemical deterioration examined. The viability loss of Vigna radiata seeds during storage is associated with Maillard reactions; however, the contribution of primary biochemical reactions varies under different storage conditions. Biochemical deterioration and viability loss are greatly retarded in seeds stored below a high critical temperature (approximately 40 degrees C above glass transition temperature). This high critical temperature corresponds to the cross-over temperature (T(c)) of glass transition where molecular dynamics changes from a solid-like system to a normal liquid system. The data show that seed ageing slows down significantly, even before seed tissue enters into the glassy state.

Glucose delays seed germination in Arabidopsis thaliana

“…Nagaraj et al 1996). In plants the Maillard reactions products are thought to influence seed colour during aging and are associated with seed viability loss depending on storage conditions (Taylor et al 2000;Murthy et al 2003). The germination delay observed could be due to such events but this is unlikely since the random process of glycoprotein formation would affect cellular processes in general.…”

Physiological-biochemical parameters and characteristics of seed coat structure in lupin seeds subjected to long storage at different temperatures

“…Nevertheless, even in seeds of high longevity, degenerative processes do occur during long storage, and they include lipid oxidation, membrane and DNA impairments, degradation of proteins and decrease of enzyme activities (Murthy et al 2003). Ageing-related reduction of enzyme activity was observed in enzymes as essential as dehydrogenases of the Krebs cycle, cytochrom c oxidase, and malate and glutamate dehydrogenases.…”

Production of reactive oxygen species in Dalbergia nigra seeds under thermal stress

“…Moreover, according to Gill and Tuteja (2010) (Figures 3 and 4). In this case, the presence of ROSs may arise from non-enzymatic reactions, such as lipid peroxidation and the Amadori and Maillard reactions since the enzyme activity of the antioxidant system is probably reduced in this condition (Murthy et al, 2003). Pukacka and Ratajczak (2005) also found hydrogen peroxide production in dry Fagus sylvatica seeds.…”

“…The high moisture content of the seeds associated with the increase in the hydrogen peroxide content compromised the germination process. Okane et al (1996) observed that H 2 O 2 production in calluses of Arabidopsis thaliana plants was greater at 4 °C than at 23 °C. Similar results were found by Sun et al (2010), who observed accumulation of H 2 O 2 in leaf tissue under low temperature conditions in Nicotiana tabacum seedlings.…”