Glucose delays seed germination in Arabidopsis thaliana

Dekkers, Bas J.W.; Schuurmans, J. A. M. J.; Smeekens, Sjef

doi:10.1007/s00425-003-1154-9

Cited by 133 publications

(121 citation statements)

References 53 publications

(80 reference statements)

Supporting

Mentioning

112

Contrasting

Unclassified

Order By: Relevance

“…e Echinacea angustifolia DC. (Felippe et al 1970, Dekkers et al 2004, Macchia et al 2001 Os resultados referentes aos efeitos do escuro e da luz branca sobre a germinação de sementes recém-coletadas de L. flilifolia, L. rotundifolia e L. sidoides são apresentados na tabela 4. Sementes de L. filifolia germinaram em percentuais mais elevados no escuro, enquanto que as sementes de L. rotundifolia germinaram mais intensamente em presença de luz e as sementes de L. sidoides germinaram em percentuais muito próximos, independente da condição testada.…”

Section: Resultsunclassified

Floração, germinação e estaquia em espécies de Lippia L. (Verbenaceae)

Pimenta¹,

Fernandes²,

Pereira³

et al. 2007

Rev. bras. Bot.

View full text Add to dashboard Cite

-(Flowering, germination and rooting of cuttings of Lippia L. (Verbenaceae)). Lippia species from Cadeia do Espinhaço (MG, Brazil), were collected and established at the Botanical Experimental Station, Juiz de Fora, MG. The flowering of plants was evaluated in both natural and controlled conditions. Germination test was accomplished with seeds obtained from natural conditions. The rooting of cuttings was evaluated in plants cultivated in the Botanical Experimental Station. The majority of species blossomed either in the dry or in the rainy seasons. Only one species blooms in both seasons. At controlled conditions, the flowering period increased in species that flourish in the summer. Some species presented better germination with fresh collected seeds while others when the seeds were stored, evidencing both viability loss and seed dormancy. GA 3 stimulates the germination in some species, while it inhibited or not influenced on others. Some species germinate better in the darkness, while others under white light. Some of them germinate in the light or in the darkness. Adventitious roots formation in cuttings of wild species was very low and did not vary in response to season variation and auxin concentration. On the other hand, rooting of cuttings of L. alba (Mill.) N.E. Br. varied in response both to season variation and to auxin types and concentration. This is the first report on physiological reproductive aspects of endemic Lippia species from the Cadeia do Espinhaço. The results indicate the possibility to use seeds in the propagation of wild Lippia species and, they also show that reproduction through conventional vegetative propagation techniques presents quite reduced efficiency.

show abstract

Section: Resultsunclassified

Floração, germinação e estaquia em espécies de Lippia L. (Verbenaceae)

Pimenta¹,

Fernandes²,

Pereira³

et al. 2007

Rev. bras. Bot.

View full text Add to dashboard Cite

show abstract

“…The susceptibility of pgn is independent of SA-, ET-, and JAmediated responses, and the mutant shows no altered resistance to virulent or avirulent strains of the bacterial pathogen P. syringae, suggesting the defense function of PGN is restricted to necrotrophic infection. However, the altered germination responses of the pgn mutant to ABA, ACC, Glc, and salt also suggest a critical role for PGN in abiotic stress tolerance (Gazzarrini and McCourt, 2001;Dekkers et al, 2004;Tuteja, 2007). Importantly, the pgn mutant accumulates increased levels of ABA and stress-induced ROS, both of which may underpin its various phenotypes.…”

Section: Discussionmentioning

confidence: 98%

“…The Glc Hypersensitivity of the pgn Mutant Is Partially Restored by ABA Antagonists Increased concentrations of Glc are known to delay Arabidopsis germination, with ABA levels determining the severity of inhibition (Gazzarrini and McCourt, 2001;Dekkers et al, 2004). Many mutants exhibiting enhanced growth on media containing high exogenous sugars are also ABA insensitive, whereas even minimal ABA increases act additively to sugar-mediated seedling growth inhibition (Laby et al, 2000;Gazzarrini and McCourt, 2001;Gibson et al, 2001).…”

Section: Pgn Encodes a Pprp Localized To Mitochondriamentioning

confidence: 99%

The Arabidopsis Mitochondria-Localized Pentatricopeptide Repeat Protein PGN Functions in Defense against Necrotrophic Fungi and Abiotic Stress Tolerance

2011

View full text Add to dashboard Cite

Pentatricopeptide repeat (PPR) proteins (PPRPs) are encoded by a large gene family in Arabidopsis (Arabidopsis thaliana), and their functions are largely unknown. The few studied PPRPs are implicated in different developmental processes through their function in RNA metabolism and posttranscriptional regulation in plant organelles. Here, we studied the functions of Arabidopsis PENTATRICOPEPTIDE REPEAT PROTEIN FOR GERMINATION ON NaCl (PGN) in plant defense and abiotic stress responses. Inactivation of PGN results in susceptibility to necrotrophic fungal pathogens as well as hypersensitivity to abscisic acid (ABA), glucose, and salinity. Interestingly, ectopic expression of PGN results in the same phenotypes as the pgn null allele, indicating that a tight regulation of the PGN transcript is required for normal function. Loss of PGN function dramatically enhanced reactive oxygen species accumulation in seedlings in response to salt stress. Inhibition of ABA synthesis and signaling partially alleviates the glucose sensitivity of pgn, suggesting that the mutant accumulates high endogenous ABA. Accordingly, induction of NCED3, encoding the rate-limiting enzyme in stress-induced ABA biosynthesis, is significantly higher in pgn, and the mutant has higher basal ABA levels, which may underlie its phenotypes. The pgn mutant has altered expression of other ABA-related genes as well as mitochondria-associated transcripts, most notably elevated levels of ABI4 and ALTERNATIVE OXIDASE1a, which are known for their roles in retrograde signaling induced by changes in or inhibition of mitochondrial function. These data, coupled with its mitochondrial localization, suggest that PGN functions in regulation of reactive oxygen species homeostasis in mitochondria during abiotic and biotic stress responses, likely through involvement in retrograde signaling.

show abstract

“…However, there is precedent for different sugar concentrations to have variable, and potentially opposite, effects. For example, high concentrations of exogenous sugars have been shown to delay seed germination (Price et al, 2003;Dekkers et al, 2004), whereas low concentrations of sugars have been shown to have a positive effect on seed germination, allowing wild-type seeds to germinate on concentrations of ABA that would otherwise be inhibitory (Garciarrubio et al, 1997;Finkelstein and Lynch, 2000). An alternative explanation for the results of assaying sugar-regulated gene expression is that mutations in SIS3 might not affect the sugar-regulated expression of these genes but might instead affect the expression of these genes via some other mechanism, leading to increased expression of the genes assayed in mutant seeds germinated in the presence of both sorbitol and Glc.…”

Section: Discussionmentioning

confidence: 99%

SUGAR-INSENSITIVE3, a RING E3 Ligase, Is a New Player in Plant Sugar Response

Huang

Pattison

et al. 2010

Plant Physiol.

View full text Add to dashboard Cite

Sugars, such as sucrose and glucose, have been implicated in the regulation of diverse developmental events in plants and other organisms. We isolated an Arabidopsis (Arabidopsis thaliana) mutant, sugar-insensitive3 (sis3), that is resistant to the inhibitory effects of high concentrations of exogenous glucose and sucrose on early seedling development. In contrast to wildtype plants, sis3 mutants develop green, expanded cotyledons and true leaves when sown on medium containing high concentrations (e.g. 270 mM) of sucrose. Unlike some other sugar response mutants, sis3 exhibits wild-type responses to the inhibitory effects of abscisic acid and paclobutrazol, a gibberellic acid biosynthesis inhibitor, on seed germination. Map-based cloning revealed that SIS3 encodes a RING finger protein. Complementation of the sis3-2 mutant with a genomic SIS3 clone restored sugar sensitivity of sis3-2, confirming the identity of the SIS3 gene. Biochemical analyses demonstrated that SIS3 is functional in an in vitro ubiquitination assay and that the RING motif is sufficient for its activity. Our results indicate that SIS3 encodes a ubiquitin E3 ligase that is a positive regulator of sugar signaling during early seedling development.Almost all living organisms rely on the products of plant photosynthesis for sustenance, either directly or indirectly. Carbohydrates, the major photosynthates, provide both energy and carbon skeletons for fungi, plants, and animals. In addition, sugars, such as Suc and Glc, function as signaling molecules to regulate plant growth, development, gene expression, and metabolic processes. Sugar response pathways are integrated with other signaling pathways, such as those for light, phytohormones, stress, and nitrogen (Dijkwel et al

show abstract

Glucose delays seed germination in Arabidopsis thaliana

Cited by 133 publications

References 53 publications

Floração, germinação e estaquia em espécies de Lippia L. (Verbenaceae)

Floração, germinação e estaquia em espécies de Lippia L. (Verbenaceae)

The Arabidopsis Mitochondria-Localized Pentatricopeptide Repeat Protein PGN Functions in Defense against Necrotrophic Fungi and Abiotic Stress Tolerance

SUGAR-INSENSITIVE3, a RING E3 Ligase, Is a New Player in Plant Sugar Response

Contact Info

Product

Resources

About