Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent

Abstract

BackgroundPropolis is a natural substance produced by bees and is known to have antimicrobial activity. Our aim was to evaluate the antimicrobial effect of micellar nanocomposites loaded with an ethyl acetate extract of Brazilian red propolis as a cavity cleaning agent and its influence on the color and microtensile bond strength (μTBS) of the dentin/resin interface.MethodsAn ultra-performance liquid chromatography coupled with a diode array detector (UPLC-DAD) assay was used to determine the flavonoids and isoflavones present in an ethyl acetate extract of Brazilian red propolis (EARP) and micellar nanocomposites loaded with EARP (MNRP). The antimicrobial activity of EARP and MNRP was tested against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. One of the following experimental treatments was applied to etched dentin (phosphoric acid, 15 s): 5 μL of MNRP (RP3, 0.3%; RP6, 0.6%; or RP1, 1.0% w/v), placebo, and 2% chlorhexidine digluconate. Single Bond adhesive (3 M/ESPE) was applied and a 4-mm-thick resin crown (Z350XT, 3 M/ESPE) was built up. After 24 h, the teeth were sectioned into sticks for the μTBS test and scanning electron microscopy. Spectrophotometry according to the CIE L*a*b* chromatic space was used to evaluate the color. Data were analyzed using one-way ANOVA and the Tukey test or Kruskal-Wallis test and the same test for pairwise comparisons between the means (P < 0.05).ResultsThe UPLC-DAD assay identified the flavonoids liquiritigenin, pinobanksin, pinocembrin, and isoliquiritigenin and the isoflavonoids daidzein, formononetin, and biochanin A in the EARP and micellar nanocomposites. EARP and MNRP presented antimicrobial activity against the cariogenic bacteria Streptococcus mutans and Lactobacillus acidophilus, and for Candida albicans. ΔE values varied from 2.31 to 3.67 (P = 0.457). The mean μTBS for RP1 was significantly lower than for the other groups (P < 0.001). Dentin treated with RP1 showed the shortest resin tags followed by RP6 and RP3.ConclusionsThe EARP and (MNRP) showed antimicrobial activity for the main agents causing dental caries (Streptococcus mutans and Lactobacillus acidophilus) and for Candida albicans. MNRP at concentrations of 0.3 and 0.6% used as a cavity cleaner do not compromise the aesthetics or μTBS of the dentin/resin interface.

Advances in Biosynthesis, Pharmacology, and Pharmacokinetics of Pinocembrin, a Promising Natural Small-Molecule Drug

“…Pinocembrin may enhance the activities of hexokinase and pyruvate kinase via the Akt/mTOR signaling pathway, thereby improving insulin resistance [63]. In daily life, given its antibacterial and antioxidant activities, pinocembrin can be used as mouth cleaning agent and sunscreen [7,64].…”

“…Biosynthesis plays an important role in the synthesis of pinocembrin owing to its high yield and low production cost. In terms of pharmacological effects, pinocembrin can exhibit anti-inflammatory [5], anti-oxidant [6], antibacterial [7] and neuroprotective activities [8]. Research has shown that pinocembrin exhibits a positive effect on the treatment of ischemic stroke.…”

Clinical and Microbiological Evaluation of Brazilian Red Propolis Containing-Dentifrice in Orthodontic Patients: A Randomized Clinical Trial

“…BRP has potent antimicrobial activity even at the concentration of 0.1%, with antimicrobial and anti-inflammatory activity proven in vitro and in vivo. is is due to the high concentration of flavonoids and phenolic compounds [14,16]. e group treated with propolis at 1% concentration in this study presented better clinical and microbiological results when compared with commercial toothpaste.…”

“…Several studies have been conducted on propolis in several areas such as Medicine, Dentistry, and Chemistry. In Dentistry, studies point to promising results in Endodontics, Cariology, Surgery, Preventive Dentistry, and Periodontics [16].…”

Red propolis hydroalcoholic extract inhibits the formation of Candida albicans biofilms on denture surface

“…The biofilm model in this study validates its effect for decontamination of dental prostheses, without contact with the oral mucosa. It is still necessary to carry out new studies using different concentrations of the product and with a longer exposure time to evaluate the effect on the color change on the surfaces of dental prostheses ( 29 ).…”