Aim. To evaluate the efficacy of dentifrice containing brazilian red propolis (BRP) in adolescents under orthodontic treatment. Materials and Methods. This is a randomized, double-blind, clinical trial. A total of 92 participants free from caries were randomized into 2 groups; the first received fluoride dentifrice, and the second received fluoride dentifrice incorporated with BRP. The gingival bleeding index (GBI) was recorded, and saliva was collected on the baseline (D0) and after 28 days (D28) for microbiological analysis. Data from GBI and Colony Forming Units (CFU) (log10) were expressed as mean and standard deviation. Results. The two groups reduced GBI significantly, with no difference in the intergroup analysis. In the intragroup analysis, it was observed that G2 (p<0.001) had a significant reduction for Gram-negative bacteria, while there was significance (p<0.001) in the intergroup analysis when compared with G1. For S. mutans bacteria, it was observed that only G2 had a statistically significant reduction (p<0.001), and there was significant reduction (p=0.006) in the intergroup analysis of the G2 group when compared with G1. Conclusions. Dentifrice containing BRP demonstrated better clinical and microbiological activity. Future studies are needed to better identify effects to establish the use of dentifrice containing propolis in biofilm control.
Background: This study evaluated the antimicrobial activity of brazilian red propolis (BRP) dentifrice and compare in vitro. Materials and methods: Strains of S. mutans ATCC UA159 were used in the present study. This study compared the antibacterial effects of BRP extract, BRP containingdentifrice and one antimicrobial commercial dentifrice (Parodontax) against S. mutans. For the evaluation of the antimicrobial activity the microdilution method was used in culture broth. The strain was activated by incubation at 37°C overnight in Brain Heart Infusion (BHI) culture medium, in an anaerobic jar. To the wells of the microplates were added 100μL of BHI broth, 20μL of the substances tested (experimental groups), at concentrations ranging from 100 μL/mL to 0.0488 μL/mL, and 80 μL/mL of the standardized microbial suspension. The microplates were incubated for 24 hr in a bacteriological oven at 37°C. Visual inspection of the colour changes and reading in BioTek microplate reader at 570nm was performed to determine the Minimum Bactericidal Concentration (MBC). Mean values and standard deviations were calculated. ANOVA followed by Dunnett's test was performed; p-value of less than 0.05 was considered significant. Results: BRP extract and BRP containing-dentifrice showed antimicrobial activity against S. mutans up to the concentration of 0.3906 μg/mL. The BRP extract and dentifrice showed bactericidal effect at a concentration of 1.5625, but was able to reduce microbial viability at a concentration of up to 0.3906. Parodontax dentifrice showed antimicrobial activity at all dilutions (p< 0.05), presenting a bactericidal effect in the same concentration of the extract (1.5625). It was concluded that all the groups observed had antimicrobial activity against S. mutans. There was no difference between the propolis extract and the common dentifrice. Conclusion: BRP in pure form and processed in a toothpaste shows some antimicrobial activity against S. mutans but less than a commercial toothpaste containing herbal extracts and sodium bicarbonate.
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