Measurement of Sterylglycosides by High Performance Liquid Chromatography with 1-Anthroylnitrile Derivatives

Murui, Tateo; Wanaka, Kou

doi:10.1271/bbb.57.614

Cited by 14 publications

(7 citation statements)

References 3 publications

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“…Separation of sterylglycosides, drevatization and HPLC analysis were carried out according to Muri et al 25) and EL-Mallah et al 26) N.B. All results recorded were the mean of three concordant replicates.…”

Section: Free (Fsg) and Acylated (Asg) Sterylglycosidesmentioning

confidence: 99%

Investigation of Liquid Wax Components of Egyptian Jojoba Seeds

El-Mallah

El-Shami

2009

J. Oleo Sci.

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Egyptian jojoba seeds newly cultivated in Ismailia desert in Egypt promoted us to determine its lipid components. Fatty alcohols, fatty acids, wax esters and sterols patterns were determined by capillary GLC whereas, tocopherols profile, isopropenoid alcohols and sterylglycosides were determined by HPLC. The Egyptian seeds are rich in wax esters (55 %) with fatty alcohols C20:1and C22:1 as major components and amounted to 43.0 % and 45.6 % respectively followed by C24:1 and C18:1(9.6 % and 1.3 % respectively). The fatty acids profile showed that C20:1 is the major constituent (60 %) followed by C18:1and C22:1 (14.5 and 11.8 % respectively) whereas C24:1 was present at low concentration amounted to 1.6 %. In addition, the Egyptian jojoba wax contained C18:2 fatty acid at a level of 8.7 %. Wax esters composition showed that the local wax had C42 and C40 esters as major components amounted to 51.1 and 30.1 % respectively. Also, it had C44 and C38 at reasonable amounts (10.0 and 6.3 % respectively). Whereas C36 and C46 were present at lower concentrations amounted to 1.4 and 1.1 respectively. The sterols analysis showed the presence of campe-, stigma-, b-sito-, and isofuco-sterol amounting to 18.4 %, 6.9 %, 68.7 %, and 6.0 % respectively. The tocopherols pattern revealed that the local seed wax contained g-tocopherol as major constituent (79.2 %) followed by a-tocopherol (20.3 %). b-tocopherol as well as dtocopherol were found as minor constituents. The isopropenoid alcohols and the sterylglycosides (free and acylated) were not detected. The wax is proposed to be used in oleo chemistry and cosmetics.

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Section: Free (Fsg) and Acylated (Asg) Sterylglycosidesmentioning

confidence: 99%

Investigation of Liquid Wax Components of Egyptian Jojoba Seeds

El-Mallah

El-Shami

2009

J. Oleo Sci.

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“…The isolation of FSG and ASG from the lipid extract can be done by column chromatography [6,7], or faster and using less solvent, by solid phase extraction (SPE) alone [8][9][10][11], or combined with preparative TLC [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…Alternatively, high performance liquid chromatography with UV detection (254 nm) was applied, after derivatization to 1-anthroylnitriles, to quantify the FSG originally present and those obtained after hydrolysis of the ASG in alkaline medium [14,15]. Murui and Siew reported the quantification of 1-anthroylnitriles of FSG with HPLC-UV and those of ASG with HPLC-fluorescence detection (Ex 370 nm, Em 470 nm) [12,13]. SG and ASG could be determined, without any hydrolysis or derivatization, by TLC with colorimetric detection and quantification (densitometer) [7].…”

Section: Introductionmentioning

confidence: 99%

Identification and Occurrence of Steryl Glucosides in Palm and Soy Biodiesel

Hoed

Zyaykina²,

Greyt³

et al. 2008

J Americ Oil Chem Soc

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A problem of excessive sedimentation was detected in soy and palm biodiesel, preventing the product from complying with requirements on contamination/ filterability. The objective of the study was to determine the nature of the sediment by different analytical techniques and to obtain data on the typical range of its components in industrially produced biodiesel samples. The sediment was investigated and the appearance of haze is linked to the presence of free steryl glucosides (FSG) above a certain concentration. This paper focuses on the original analytical approach, taking into account particular physical properties of FSG. Nuclear magnetic resonance and mass spectrometry were used as fast and reliable identification methods, without the need for a prior hydrolysis of the glucosidic bond. A GC method, including optimised sample preparation, was developed for the quantification of the FSG in biodiesel as well as in filter residues. The FSG concentrations in biodiesel produced by different processes ranged between 55 and 275 mg/kg for palm and from not detectable to 158 mg/kg for soy biodiesel.

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“…The procedure of separation of free and acylated sterylglycosides from the treated byproducts, their derivatization into sterylglycosides 1-anthroylnitrile (SG-1-AN) ,HPLC analysis and the identification of the constituents were carried out according to Murui and Wanaka, 1993;El-Mallah et al, 1994a and1999b. The free sterylglycosides (FSG) and acylated sterylglycosides(ASG) were separated from the treated by-product by injecting the sample solution (100mg) into a silica gel cartridge (SepPak, Waters) and were eluted with chloroform: methanol (1:1v/v).The mixed compounds were subsequently divided into FSG and ASG with the help of a preparative TLC (Wako 70 plate). Chloroform/methanol /formic acid mixture (90/15/1, v/v/v) was used as the developing solvent (ASG fraction has an Rf of 0.5 whereas the Rf of FSG is 0.3).…”

Section: Free and Acylated Sterylglycosidesmentioning

confidence: 99%

HPLC evaluation of the minor lipid components of by-products resulting from edible oil processing

El-Mallah¹,

Hassanein²,

El-Shami³

2006

Grasas y Aceites

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RESUMEN Evaluación por HPLC de componentes lipídicos minoritarios de subproductos de la refinación de aceites comestiblesEl trabajo realiza una evaluación analítica de algunos subproductos resultantes del la refinación de aceites comestibles. Las muestras procedieron de 4 plantas que aplicaban refinación química. Después de un pretratamiento de las muestras estas se sometieron a un análisis cromatográ-fico para el aislamiento y derivatización de los siguientes componentes minoritarios: esteroles libres y acilados (FS y AS) y esterilglicosidos libres y acilados (FSG y ASG). Sin embargo, los tocoferoles se determinaron directamente a partir de las muestras pretratadas. Todos los componentes menores se analizaron mediante HPLC, utilizando detectores apropiados.Algunos autores han estudiado los esteroles y los esteres de los esteroles en los subproductos de la refinación pero ninguno lo ha hecho hasta ahora con los glicosidos de los esteroles, que son son de gran importancia biológica.Este estudio aporta información sobre subproductos enriquecidos con ciertos componentes que pueden ser una fuente interesante de los mismos. En el trabajo se discute asimismo el papel de las condiciones de refinación sobre la eliminación de dichos componentes de los aceites tratados. Se ha encontrado que los jabones contienen cantidades variables de tocoferoles que oscilan entre 80 y 230 ppm; la cantidad total de AS y FS osciló entre 240 a 4000 mg/100g mientras que la concentración total de FSG y ASG varió entre 1120 a 6375 mg/100g. En caso de desodorización, los destilados contuvieron entre 960 to 7360ppm de tocoferol, mientras que el total de FS y AS se situó entre 1020 y 4160 mg/100g y el de los totales de FSG y ASG entre 395 y 880 mg/100g. PALABRAS-CLAVE: Análisis mediante HPLC -Esteroles acilados -Esteroles libres -Esterolglicosidos -Subproductos -Tocoferoles. SUMMARY HPLC evaluation of the minor lipid components of by-products resulting from edible oil processingAn analytical evaluation of some by-products resulting from edible oil refining processing steps has been carried out. By-product samples were taken from four different local refineries that apply chemical refining technology. Pretreatment of the representative samples of the by-products were done prior to analysis followed by chromatographic isolation and derivatization of the minor components, namely, free and acylated sterol (FS and AS) as well as free and acylated sterylglycosides (FSG and ASG). However, tocopherols were directly determined in the pretreated samples. HPLC, using HPLC evaluation of the minor lipid components of by-products resulting from edible oil processing This study throws light on the by -products enriched with certain minor components to be possibly utilized as sources for such components. Also, the role of the conditions of the refining steps followed in removing these valuable minor components from oils was discussed. It was found that soapstock samples contained various amounts of total tocopherols ranging from 80 to 230ppm; total FS and AS ra...

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Measurement of Sterylglycosides by High Performance Liquid Chromatography with 1-Anthroylnitrile Derivatives

Cited by 14 publications

References 3 publications

Investigation of Liquid Wax Components of Egyptian Jojoba Seeds

Investigation of Liquid Wax Components of Egyptian Jojoba Seeds

Identification and Occurrence of Steryl Glucosides in Palm and Soy Biodiesel

HPLC evaluation of the minor lipid components of by-products resulting from edible oil processing

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