Measurement of phosphate in small samples using capillary electrophoresis with laser-induced luminescence detection

Lischynski, Jennifer R; Goltz, Douglas M.; Craig, Douglas B.

doi:10.1080/10826076.2018.1564326

Journal of Liquid Chromatography & Related Technologies

2018

DOI: 10.1080/10826076.2018.1564326

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Measurement of phosphate in small samples using capillary electrophoresis with laser-induced luminescence detection

Jennifer R Lischynski

Douglas M. Goltz

Douglas B. Craig

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Cited by 3 publications

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“…Curretnly, several analytical methods have been developed for the determination of phosphate in biological samples, including electrochemical, chromatographic, electrokinetic, colorimetric, and fluorometric techniques. Among them, methods based on luminescence can serve as a simple approach for phosphate quantification with particular advantages with respect to other analytical techniques because of their high specificity, separation-free detection characteristic, and robustness against matrix interferences .…”

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confidence: 99%

A Turn-On Luminescence Method for Phosphate Determination Based on Fast Green-Functionalized ZrO₂:Yb,Er@ZrO₂ Core@Shell Upconversion Nanoparticles

et al. 2019

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The development of practical and sensitive tools for detecting phosphate deficiency could facilitate engineering approaches to enhance crop yield and quality in phosphate-stressed environments, reducing the misuse of nonrenewable fertilizers and their consequent ecological impact. Herein, a 975 nm-activated method based on ZrO2:Yb,Er@ZrO2 core@shell upconversion nanoparticles is presented for rapid visualization and determination of the phosphate ions in aqueous solutions and extracts. At optimized thickness, the nondoped ZrO2 shell not only enhances the emission of the ZrO2:Yb,Er but also provides an active surface for the intense interaction with the phosphate group, allowing a “label-free” determination that avoids the use of additional phosphate-recognizing elements like ligands or antibodies. According to the experimental evidence, the optical output of the ZrO2:Yb,Er@ZrO2 nanoparticles specifically matches with the absorption spectrum of the fast green alimentary dye (FG) electrostatically attached to the nanoparticle surface, activating the Förster resonance energy transfer (FRET) and thereby the upconversion luminescence quenching. Upon addition of the phosphate ions and the covalent interaction with the ZrO2:Yb,Er@ZrO2–FG nanocomplex, the FG is gradually removed, displaying a fast and reproducible “turn-on” luminescence which allows measurements in a few minutes. This rapid response is due to the stronger coordination between the ZrO2 shell and the phosphate compared to the FG molecules (−31.97 and −5.99 eV, respectively). The detection method was then effectively modulated in a 20–1000 nM linear response range without interfering effects of commonly coexisting ions, achieving a detection limit up to 15 times lower than that obtained with the conventionally used colorimetric methods.

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mentioning

confidence: 99%

A Turn-On Luminescence Method for Phosphate Determination Based on Fast Green-Functionalized ZrO₂:Yb,Er@ZrO₂ Core@Shell Upconversion Nanoparticles

et al. 2019

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Simple, fast, and simultaneous determination of orthophosphate, pyrophosphate, and tripolyphosphate by capillary electrophoresis with capacitively coupled contactless conductivity detection

Barboza,

Rocha,

de Jesus

2024

Electrophoresis

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This work describes a novel analytical method using capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C4D) for simultaneous, simple, and rapid determination of three inorganic phosphates (orthophosphate, pyrophosphate, and tripolyphosphate) widely used as food additives and in pharmaceutical formulations. A background electrolyte composed of 0.5 mol L–1 acetic acid provided fast separation (around 3.0 min) and good separation efficiency and peak resolution. Linearity in the concentration range of 10–500 mg L–1 was confirmed by the coefficients of determination (R2) higher than 0.99. The limits of detection varied from 0.41 to 0.58 mg L–1. The accuracy of the proposed method was assessed by recovery tests conducted at three concentration levels in tap water samples, food, and personal hygiene products. Recovery values varying from 81% to 118% were achieved, indicating an acceptable accuracy. The proposed CE‐C4D successfully determined the three inorganic phosphates in the analyzed samples.

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Automated online monitoring of lactate and pyruvate in tamoxifen resistant MCF-7 cells using sequential-injection capillary electrophoresis with contactless conductivity detection (SI-CE-C⁴D) and correlation with MCT1 and MCT4 genes expression

Alhusban

Hamadneh

Shallan

et al. 2022

Journal of Liquid Chromatography & Related Technologies

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Measurement of phosphate in small samples using capillary electrophoresis with laser-induced luminescence detection

Cited by 3 publications

References 32 publications

A Turn-On Luminescence Method for Phosphate Determination Based on Fast Green-Functionalized ZrO₂:Yb,Er@ZrO₂ Core@Shell Upconversion Nanoparticles

A Turn-On Luminescence Method for Phosphate Determination Based on Fast Green-Functionalized ZrO₂:Yb,Er@ZrO₂ Core@Shell Upconversion Nanoparticles

Simple, fast, and simultaneous determination of orthophosphate, pyrophosphate, and tripolyphosphate by capillary electrophoresis with capacitively coupled contactless conductivity detection

Automated online monitoring of lactate and pyruvate in tamoxifen resistant MCF-7 cells using sequential-injection capillary electrophoresis with contactless conductivity detection (SI-CE-C⁴D) and correlation with MCT1 and MCT4 genes expression

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Measurement of phosphate in small samples using capillary electrophoresis with laser-induced luminescence detection

Cited by 3 publications

References 32 publications

A Turn-On Luminescence Method for Phosphate Determination Based on Fast Green-Functionalized ZrO2:Yb,Er@ZrO2 Core@Shell Upconversion Nanoparticles

A Turn-On Luminescence Method for Phosphate Determination Based on Fast Green-Functionalized ZrO2:Yb,Er@ZrO2 Core@Shell Upconversion Nanoparticles

Simple, fast, and simultaneous determination of orthophosphate, pyrophosphate, and tripolyphosphate by capillary electrophoresis with capacitively coupled contactless conductivity detection

Automated online monitoring of lactate and pyruvate in tamoxifen resistant MCF-7 cells using sequential-injection capillary electrophoresis with contactless conductivity detection (SI-CE-C4D) and correlation with MCT1 and MCT4 genes expression

Contact Info

Product

Resources

About

A Turn-On Luminescence Method for Phosphate Determination Based on Fast Green-Functionalized ZrO₂:Yb,Er@ZrO₂ Core@Shell Upconversion Nanoparticles

A Turn-On Luminescence Method for Phosphate Determination Based on Fast Green-Functionalized ZrO₂:Yb,Er@ZrO₂ Core@Shell Upconversion Nanoparticles

Automated online monitoring of lactate and pyruvate in tamoxifen resistant MCF-7 cells using sequential-injection capillary electrophoresis with contactless conductivity detection (SI-CE-C⁴D) and correlation with MCT1 and MCT4 genes expression