Automated online monitoring of lactate and pyruvate in tamoxifen resistant MCF-7 cells using sequential-injection capillary electrophoresis with contactless conductivity detection (SI-CE-C⁴D) and correlation with MCT1 and MCT4 genes expression

Glutathione and Xanthine Metabolic Changes in Tamoxifen Resistant Breast Cancer Cell Lines are Mediated by Down-Regulation of GSS and XDH and Correlated to Poor Prognosis

“…MCF-7 cell lines (ATCC, USA) were cultured in RPMI 1640 media (EuroClone S.p.A., Italy) supplemented with 1% penicillin-streptomycin, 1% L-Glutamine, and 10% fetal bovine serum (FBS) and incubated in a 37 °C incubator under 5% CO2 atmosphere. Three tamoxifen-resistant MCF7 cell lines were developed using 3 different tamoxifen concentration approaches as previously described 21 - 23 . The first approach involved treating the cells with increasing tamoxifen concentrations starting with 100 nM until a concentration of 50 µM was reached.…”

Application of capillary electrophoresis with capacitively contactless conductivity detection for biomedical analysis

“…Therefore, the determination formate in blood and urinary oxalate and citrate is very important for diagnosis and management of diseases. CE-C 4 D has been demonstrated as a satisfactory method for the determination of various types of small organic ions, such as oxalate, citrate, [77], lactate [78][79][80], lactate and pyruvate [81,82], formate [83][84][85], oxalate, formate, and glycolate [86], and fluoroacetate [87]. The analyses of organic acids such as γ-hydroxybutyric acid in urine and serum [88], valproic acid (VPA) [89], organic acids in urine [90], uric acid in plasma and human urine [91], Ghydroxybutyric acid, ibotenic acid, and psilocybin in saliva [92], and urinary ethylmalonic acid [93] employing CE-C 4 D were also reported.…”

“…The analysis of lactate formation in culture cell using sequential injection‐CE (SI–CE) with C 4 D) was presented by Alhusban et al. [81]. Optimized BGE consisted of 25 m m (Tris), 35 m m cyclohexyl‐2‐aminoethanesulfonic acid (CHES) with 0.02% polyethyleneimine at pH 8.65 was used to separate lactate in the cell media.…”

Rapid and sensitive HPLC–MS/MS method for the quantification of dopamine, GABA, serotonin, glutamine and glutamate in rat brain regions after exposure to tobacco cigarettes

“…Several techniques have been used for the measurement of neurotransmitters in brain tissues. For example, capillary electrophoresis provides high resolution power (Alhusban et al, 2017; Alhusban, Hamadneh, Albustanji, & Shallan, 2022; Alhusban, Hamadneh, Shallan, & Tarawneh, 2022; Hamadneh et al, 2021) and has been applied successfully for the quantification of several neurotransmitters in brain tissues (Bowser & Kennedy, 2001; Denoroy et al, 2008; Hua Li et al, 2010; Hua Li & Yan, 2010; Sauvinet et al, 2003). However, capillary electrophoresis has low sensitivity as one of its limitations.…”