Matrix‐induced autologous chondrocyte implantation in sheep: objective assessments including confocal arthroscopy

Jones, Christopher W.; Willers, Craig; Keogh, Angus; Smoliński, Dariusz Jan; Fick, Daniel; Yates, Piers; Kirk, T.B.; Zheng, Minghao

doi:10.1002/jor.20502

Cited by 64 publications

(56 citation statements)

References 32 publications

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“…Sheep have successfully been used for implantation of autologous chondrocytes on a collagen matrix [13] and for chondrocytes and MSCs embedded in gels [10][11][12][14][15][16]. Godoy et al (2014) used sheep to compare stem cells from bone marrow, adipose tissue and synovium [17].…”

Section: Introductionmentioning

confidence: 99%

Perivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous Transplantation in a Model of Articular Cartilage Repair

Hindle

Baily

Khan

et al. 2016

Stem Cells and Development

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Publisher Rights Statement:This is the final peer-reviewed manuscript as accepted for publication General rightsCopyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Take down policyThe University of Edinburgh has made every reasonable effort to ensure that Edinburgh Research Explorer content complies with UK legislation. If you believe that the public display of this file breaches copyright please contact openaccess@ed.ac.uk providing details, and we will remove access to the work immediately and investigate your claim. Immunohistochemistry and fluorescence-activated cell sorting (FACS) were used to ascertain the reactivity of anti-human and anti-ovine antibodies, which were combined and used to identify and isolate pericytes (CD34-CD45-CD146+) and adventitial cells (CD34+CD45-CD146-). The purified cells demonstrated osteogenic, adipogenic and chondrogenic potential in culture.Autologous ovine PSCs (oPSCs) were isolated, cultured and efficiently transfected using a GFP encoding lentivirus. The cells were implanted into articular cartilage defects on the medial femoral condyle using a hydrogel and collagen membranes.Four weeks following implantation the condyle was explanted and confocal laser scanning microscopy demonstrated the presence of oPSCs in the defect repaired with the hydrogel.These data suggest the testability in a large animal of native mesenchymal stem cell autologous grafting, thus avoiding possible biases associated with xenotransplantation.Such a setting will be used in priority for indications in orthopaedics, at first to model articular cartilage repair.

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Section: Introductionmentioning

confidence: 99%

Perivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous Transplantation in a Model of Articular Cartilage Repair

Hindle

Baily

Khan

et al. 2016

Stem Cells and Development

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“…The membrane functions as a barrier for the recovery area and as an adhesion site for cells. Thus, it can support the regeneration of the damaged tissue [95].…”

Section: Commercial Productsmentioning

confidence: 99%

Hydrogels based on collagen and fibrin – frontiers and applications

Schneider-Barthold¹,

Baganz²,

Wilhelmi

et al. 2016

BioNanoMaterials

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Hydrogels are a versatile tool for a multitude of applications in biomedical research and clinical practice. Especially collagen and fibrin hydrogels are distinguished by their excellent biocompatibility, natural capacity for cell adhesion and low immunogenicity. In many ways, collagen and fibrin represent an ideal biomaterial, as they can serve as a scaffold for tissue regeneration and promote the migration of cells, as well as the ingrowth of tissues. On the other hand, pure collagen and fibrin materials are marked by poor mechanical properties and rapid degradation, which limits their use in practice. This paper will review methods of modification of natural collagen and fibrin materials to next-generation materials with enhanced stability. A special focus is placed on biomedical products from fibrin and collagen already on the market. In addition, recent research on the in vivo applications of collagen and fibrin-based materials will be showcased.

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“…In this technique, harvested chondrocytes are cultured on a bio absorbable, porcine Type-I/III collagen membrane before implantation into the defect [42]. More cell distribution, performing the technique arthroscopically, preventing the dedifferentiation of chondrocytes.…”

Section: Second Generation Autologous Chondrocyte Implantationmentioning

confidence: 99%

Talar Osteochondral Defects

Görmeli¹,

Ersoy²,

Görmeli³

2015

Med-Science

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Intra-articular ankle injuries to the talar body is one of the common cause of athletic disability that have been estimated to occur in approximately 6.5% of the ankle sprains and commonly affect the dome of the talus. Patients are commonly referred to the clinics with an acute injury to the ankle or with chronic ankle instability. This article describes the clinical presentation and imaging to diagnose of talar osteochondral defects. The treatment optionsare also reviewed to give consensus on optimal treatment of this condition.

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Matrix‐induced autologous chondrocyte implantation in sheep: objective assessments including confocal arthroscopy

Cited by 64 publications

References 32 publications

Perivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous Transplantation in a Model of Articular Cartilage Repair

Perivascular Mesenchymal Stem Cells in Sheep: Characterization and Autologous Transplantation in a Model of Articular Cartilage Repair

Hydrogels based on collagen and fibrin – frontiers and applications

Talar Osteochondral Defects

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