Hydrogels based on collagen and fibrin – frontiers and applications

Schneider-Barthold, Caroline; Baganz, Sabrina; Wilhelmi, Mathias; Scheper, Thomas; Pepelanova, Iliyana

doi:10.1515/bnm-2015-0025

Cited by 47 publications

(25 citation statements)

References 94 publications

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“…To conclude this first part, it is important to point out some common features and major differences between these two kinds of hydrogels that would impact not only the conditions of preparation of mixed systems but also allow to anticipate some of their structural or biological features [ 108 ]. A first point to emphasize is related to the fact that in the case of type I collagen, the usual form used for hydrogel formation is the tropocollagen molecule, i.e., a form that has already been converted from the inactive procollagen state by cleavage of some terminal peptides.…”

Section: A Short Presentation Of Type I Collagen and Fibrinmentioning

confidence: 99%

Type I Collagen-Fibrin Mixed Hydrogels: Preparation, Properties and Biomedical Applications

Coradin

Wang

Law

et al. 2020

Gels

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Type I collagen and fibrin are two essential proteins in tissue regeneration and have been widely used for the design of biomaterials. While they both form hydrogels via fibrillogenesis, they have distinct biochemical features, structural properties and biological functions which make their combination of high interest. A number of protocols to obtain such mixed gels have been described in the literature that differ in the sequence of mixing/addition of the various reagents. Experimental and modelling studies have suggested that such co-gels consist of an interpenetrated structure where the two proteins networks have local interactions only. Evidences have been accumulated that immobilized cells respond not only to the overall structure of the co-gels but can also exhibit responses specific to each of the proteins. Among the many biomedical applications of such type I collagen-fibrin mixed gels, those requiring the co-culture of two cell types with distinct affinity for these proteins, such as vascularization of tissue engineering constructs, appear particularly promising.

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Section: A Short Presentation Of Type I Collagen and Fibrinmentioning

confidence: 99%

Type I Collagen-Fibrin Mixed Hydrogels: Preparation, Properties and Biomedical Applications

Coradin

Wang

Law

et al. 2020

Gels

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“…Для контроля над процессом деградации фибрина используют два подхода: дополнительная поперечная сшивка нитей фибрина и применение ингибиторов фибринолиза [31]. Дополнительная поперечная сшивка нитей фибрина достигается с помощью XIII фактора, который кроме латерального ковалентного связывания между γ-и α-цепями фибрина осуществляет перекрестное сшивание с фибрином таких молекул как α2-антиплазмин, TAFI (активируемый тромбином ингибитор фибринолиза), PAI-2 (ингибитор активации плазминогена 2 типа), что стабилизирует фибриновую сеть и повышает устойчивость сшитого фибрина к фибринолизу [32,33].…”

Section: естественная и контролируемая биодеградация фибринаunclassified

Fibrin – a promising material for vascular tissue engineering

Матвеева

Khanova

Антонова

et al. 2020

RJTAO

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This review looks at the use of fibrin in vascular tissue engineering (VTE). Autologous fibrin is one of the most affordable biopolymers because it can be obtained from peripheral blood by simple techniques. A description and comparative analysis of the methods and approaches for producing fibrin gel is provided. The ability of fibrin to promote cell attachment and migration, survival and angiogenesis, to accumulate growth factors and release them in a controlled manner, are unique and extremely useful in VTE. Fibrin gels can serve as a three-dimensional matrix molded in different sizes and shapes to be applied in a variety of ways, including as a scaffold, coating, or impregnation material. Fibrin’s high porosity and biodegradability allows controllable release of growth factors, yet fibrinolysis must be tightly regulated to avoid side effects. We discuss the main methods of regulating the rate of fibrinolysis, as well as possible side effects of such exposure. Low mechanical strength is the main limitation in using fibrin as a scaffold for vascular tissue engineering. Possible options for increasing the strength properties of fibrin matrix and evaluating their effectiveness are presented. We propose that unique biocompatibility and ideal biodegradation profile of fibrin justify its use as a scaffold material for developing an ideal fully autologous small-diameter tissue-engineered vascular graft.

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“…Collagen is distinguished being a highly biocompatible cellular growth supporter and is noncytotoxic 44 . Though collagen has been extensively used in the clinic and tissue engineering for various applications 45 , its high degradation degree, mechanical instability, and thermosensitivity have led researchers to add other polymers or cross-linkers to achieve more desirable features 40 , 46 . Therefore, in this study, we chose a cross-linking approach to improve hydrogel stability while preserving the utility of collagen hydrogel as an appropriate three-dimensional (3D) structure for enhancing cell proliferation and differentiation.…”

Section: Introductionmentioning

confidence: 99%

Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel

Mahmoodi

Hassannejad

et al. 2021

Sci Rep

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Spinal cord regeneration is limited due to various obstacles and complex pathophysiological events after injury. Combination therapy is one approach that recently garnered attention for spinal cord injury (SCI) recovery. A composite of three-dimensional (3D) collagen hydrogel containing epothilone B (EpoB)-loaded polycaprolactone (PCL) microspheres (2.5 ng/mg, 10 ng/mg, and 40 ng/mg EpoB/PCL) were fabricated and optimized to improve motor neuron (MN) differentiation efficacy of human endometrial stem cells (hEnSCs). The microspheres were characterized using liquid chromatography-mass/mass spectrometry (LC-mas/mas) to assess the drug release and scanning electron microscope (SEM) for morphological assessment. hEnSCs were isolated, then characterized by flow cytometry, and seeded on the optimized 3D composite. Based on cell morphology and proliferation, cross-linked collagen hydrogels with and without 2.5 ng/mg EpoB loaded PCL microspheres were selected as the optimized formulations to compare the effect of EpoB release on MN differentiation. After differentiation, the expression of MN markers was estimated by real-time PCR and immunofluorescence (IF). The collagen hydrogel containing the EpoB group had the highest HB9 and ISL-1 expression and the longest neurite elongation. Providing a 3D permissive environment with EpoB, significantly improves MN-like cell differentiation and maturation of hEnSCs and is a promising approach to replace lost neurons after SCI.

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Hydrogels based on collagen and fibrin – frontiers and applications

Cited by 47 publications

References 94 publications

Type I Collagen-Fibrin Mixed Hydrogels: Preparation, Properties and Biomedical Applications

Type I Collagen-Fibrin Mixed Hydrogels: Preparation, Properties and Biomedical Applications

Fibrin – a promising material for vascular tissue engineering

Improving motor neuron-like cell differentiation of hEnSCs by the combination of epothilone B loaded PCL microspheres in optimized 3D collagen hydrogel

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