Marmoricola korecus sp. nov.

Lee, Soon Dong; Lee, Dong Wan; Ko, Young-Hwan

doi:10.1099/ijs.0.025460-0

Cited by 24 publications

(11 citation statements)

References 18 publications

(27 reference statements)

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“…A bioactive compound, xiamycin D isolated from Streptomyces sp (#HK18) culture inhabiting the topsoil in a Korean solar saltern was tested for antiviral activity against porcine epidemic diarrhea virus (PEDV) and results showed that xiamycin D had the strongest inhibitory effect on PEDV replication (13). Methylelaiophylin, an antiviral compound isolated from Streptomyces melanosporofaciens showed antiviral activity against Newcastle disease virus (NDV) (14).…”

Section: Introductionmentioning

confidence: 99%

Antiviral viral compound fromStreptomyces ghanaensislike strain against white spot syndrome virus (WSSV) of shrimp

Rajkumar¹,

Manimaran

Taju³

et al. 2018

Preprint

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35Actinomycetes isolates collected from different environments were screened for 36 antiviral activity against WSSV. One isolate designated as CAHSH-2 showed antiviral 37 activity against WSSV at the concentration of 0.2 mg per shrimp. The laboratory trial of 38 determining antiviral activity of ethyl acetate extract (EtOAcE) of CAHSH-2 against WSSV 39 was carried out 21 times since 2014. CAHSH-2 isolate which showed antiviral activity was 40 characterized and identified as Streptomyces ghanaensis like strain. Among the five fractions 41 obtained from EtOAcE of potential actinomycetes isolate, F1 was found to have strong 42 antiviral activity. The F1A and F1B sub-fractions from F1 fraction were subjected to GC-MS, 43 FTIR, 1 H and 13 C NMR analyses and, the compounds identified were di-n-octyl phthalate and 44 bis (2-methylheptyl) phthalate, respectively. Among these compounds, di-n-octyl phthalate 45 showed strong antiviral activity against WSSV. Molecular docking studies revealed that di-n-46 octyl phthalate was found to have high binding affinity with VP26 and VP28 proteins of 47 WSSV, whereas the bis (2-methylheptyl) phthalate showed low binding affinity with VP26 48 and VP28. The antiviral activity of EtOAcE of actinomycetes against WSSV was confirmed 49 by PCR, RT-PCR, Western blot and ELISA. The EA extract of active isolate was found to be 50 non-toxic to Artemia, post-larvae and adult Litopenaeus vannamei. 51 Importance 52 53White spot syndrome virus (WSSV) is an important shrimp viral pathogen and 54 responsible for huge economic loss to shrimp culture industry worldwide including India. The 55 global loss due to WSSV has been estimated about USD 10 billion and the loss continues at 56 the same extent even now. Various strategies have been followed to prevent or control 57 diseases of aquatic animals. In spite of various preventive and control strategies, WSSV has 58 been still persisting for more than two decades. No control strategies have so far been evolved 59 to put a break to WSSV. In this situation, an attempt was made in the present work to screen 60 some actinomycetes isolates for antiviral activity against WSSV. Among these isolates, one 61 isolate identified as Streptomyces ghanaensis like isolate CAHSH-2 showed activity against 62 WSSV. This article gives the information about the antiviral compound against WSSV and 63 the mechanism of viral inhibition. 64 65 66

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Section: Introductionmentioning

confidence: 99%

Antiviral viral compound fromStreptomyces ghanaensislike strain against white spot syndrome virus (WSSV) of shrimp

Rajkumar¹,

Manimaran

Taju³

et al. 2018

Preprint

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“…In "Djendli" sebkha, there are three genera: Marmoricola, Phytoactinopolyspora, Streptomyces, and an unclassified actinobacterium (DJ77). Most clones are uncultured (over 70%).The genus Marmoricola comes from several places in the world such as the agricultural (M. pocheoensis) and forest soils (Dastager et al 2008;Lee et al 2016), the marine environment (Maszenan et al 1999), marble (Urzì et al 2000), and volcanic ashes (Lee et al 2011). The genus Phytoactinopolyspora was recently discovered in China.…”

Section: Discussionmentioning

confidence: 99%

Diversity of actinobacteria in the marshes of Ezzemoul and Djendli in northeastern Algeria

Smati

Kitouni

2019

European Journal of Ecology

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The main purpose of this research is to study the microbial diversity of actinobacteria, living in "Ezzemoul" and "Djendli" sebkhas soils. These salt lakes are situated in the east of Algeria and they are microbiologically underexploited. Such unexplored ecological niches have been considered by many authors as sources of novel actinobacteria and bioactive molecules. Actinobacteria play an important role in safeguarding the environment by improving plant growth through nitrogen fixation, biodegradation, and bioremediation. Therefore, studying the diversity and distribution of actinobacteria in such special environments is important for determining the ecological and biotechnological roles of these microorganisms. In this article, we focused on the occurrence and the diversity of actinobacteria from sebkhas using two techniques: cultural and culture-independent (molecular cloning). The latter are based on phylogenetic analysis of the 16S rDNA gene. Thus, the cultural method allowed us to obtain 62 isolates: 40 from the "Ezzemoul" site and 22 from the "Djendli" site. These isolates tolerate mainly 2, 5, and 10% sodium chloride (NaCl) and belong to the genera Nocardiopsis, Streptomyces, and Rhodococcus. Moreover, the molecular cloning gave us 39 clones. Twenty-four clone sequences from "Ezzemoul" site are affiliated to the genera Demequina, Plantactinospora, Friedmanniella, and Mycobacterium. Also, 15 clone sequences from "Djendli" site are related to the genera Marmoricola, Phytoactinopolyspora, Streptomyces, and to an unclassified actinobacterial clone. Some sequences from both sites are related to uncultured clones. In addition to the data provided by the cultural method, molecular cloning allowed us to have additional information about the unknown actinobacteria, uncultured ones as well as on the genera that exist in both sites. So, the cultural method is complementary to the culture-independent one, and their combination revealed an important diversity in targeted saline environments. Furthermore, all new isolated strains that tolerate 10% NaCl may have a very interesting biotechnological potential in the future. ABSTRACT Actinobacteria; sebkha; culture; cloning; 16S rRNA gene; phylogenetic biodiversity KEYWORDS

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“…the polar lipid profile of strain MUSC 201 T contained a phosphoglycolipid and a glycolipid that was not detected in any of the other genera. For the fatty acids profile, strain MUSC 201 T was significantly different from Prauser, 1976;O'Donnell et al, 1982;Collins et al, 1989;Tamura & Yokota, 1994;Park et al, 1999;Dastager et al, 2009;Cho et al, 2010); 3, Aeromicrobium (Miller et al, 1991;Tamura & Yokota, 1994;Kim et al, 2010); 4, Marmoricola (Urzì et al, 2000;Dastager et al, 2008;Lee et al, 2011). DPG, diphosphatidylglycerol; GL, glycolipid; PG, phosphatidylglycerol; PGL, phosphoglycolipid; PI, phosphatidylinositol; PIM, phosphatidylinositol mannoside; PC, phosphatidylcholine; PL, unknown phospholipid.…”

Section: Strain Musc 201mentioning

confidence: 99%

Mumia flava gen. nov., sp. nov., an actinobacterium of the family Nocardioidaceae

Lee¹,

Zainal

Azman³

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Mumia flava gen. nov., sp. nov., an actinobacterium of the family Nocardioidaceae T formed a distinct clade in the family Nocardioidaceae and was most closely related to the members of the genera Nocardioides (16S rRNA gene sequence similarity, 91.9-95.1 %), Aeromicrobium (92.7-94.6 %), Marmoricola (92.5-93.1 %) and Kribbella (91.5-92.4 %). The cells of this strain were irregular coccoid to short rod shaped. The peptidoglycan contained LL-diaminopimelic acid as diagnostic diamino acid and the peptidoglycan type was A3c. The peptidoglycan cell wall contained LL-diaminopimelic acid, glycine, glutamic acid and alanine in a molar ratio of 1.5 : 0.9 : 1.0 : 1.5. The cell-wall sugars were galactose and rhamnose. The predominant menaquinone was MK-9(H 4 ). The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphoglycolipid, glycolipid and four unknown phospholipids. The major cellular fatty acids were C 18 : 1 v9c (30.8 %), C 16 : 0 (24.1 %), and 10-methyl C 18 : 0 (13.9 %). The DNA G+C content was 72.0±0.1 mol%. On the basis of phylogenetic and phenotypic differences from members of the genera of the family Nocardioidaceae, a novel genus and species, Mumia flava gen. nov., sp. nov. are proposed.

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Marmoricola korecus sp. nov.

Cited by 24 publications

References 18 publications

Antiviral viral compound fromStreptomyces ghanaensislike strain against white spot syndrome virus (WSSV) of shrimp

Antiviral viral compound fromStreptomyces ghanaensislike strain against white spot syndrome virus (WSSV) of shrimp

Diversity of actinobacteria in the marshes of Ezzemoul and Djendli in northeastern Algeria

Mumia flava gen. nov., sp. nov., an actinobacterium of the family Nocardioidaceae

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