Mapping Growth Hormone Gene of Body Weight Krui Cattle in Pesisir Barat Regency Lampung, Indonesia

Abstract

Background and Objective: The growth hormone (GH) gene plays a role in meat growth and has been shown to increase the growth rate and carcass composition after being given GH. For this function, this gene is used as a strong candidate for genetic markers for meat growth traits. The research objective was to map the growth hormone (GH) gene of the bodyweight of Krui cattle in the Pesisir Barat Regency. Materials and Methods: This research used 30 blood samples of 30 Krui cattle. The method used was by taking quantitative data and blood samples from adult Krui cattle in Pesisir Barat Regency and then the blood samples were analyzed by DNA isolation method. PCR amplification used was a pair of GH-Forward primers: 5 'ATC CAC ACC CCC TCC ACA CAGT 3' and GH-reverse: 5 'CAT TTT CCA CCC TCC CCT ACA G 3', as well as digestion using the RFLP method at the Laboratory of Animal Breeding and Genetics of Universitas Gadjah Mada, Yogyakarta. Association between genotype and body weight was analyzed descriptively. Results: The results showed that Krui cattle had polymorphic genes with three genotypes found, namely: CC, CT and TT. Cattle with CT genotype had the largest average body weight or meat production compared to those with other genotypes. Conclusion: These results indicated that the GH gene identifier has strong evidence that it can be used as a selection tool with the help of genotypes of body weight traits of Krui meat production in the Pesisir Barat Regency. Krui cattle with CT genotype can be developed further because it has high economic value with high average body weight and meat production.

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