Mango cv. 'Rataul No. 12' is one of Pakistan's finest mango cultivars. It produces small to mediumsized fruits that are flavorful, fragrant, and well-suited for preserving. Assessment of the pre-harvest amino acids application on the postharvest life of mangoes cv. 'Rataul No. 12' was done. The effect of amino acids on mango cultivar 'Rataul No. 12' with the objective of studying and improving fruit retention, yield, or qualitative and quantitative changes in physio-chemical characteristics during ripening. The impact of spraying amino acids on fruit retention at various fruiting stages i.e., pea, marble, egg, at matures stages as well as yield at the end of harvesting as compared to the control. Increasing amino acid percentages (Promise 6.55%, Flagon 10.00%, and Izabion 62.55%) also increased the number of fruits at the fully mature stage i.e., 1.43%, 1.82%, and 2.20%, respectively. As compared to control, there were statistically significant differences. The increased the percentage of amino acids also increased the yield, Izabi on was 105.9kg/plant, whereas Promise and Flagon showed 96.65kg/plant and 95.86%, but both were non-significantly different. Fruit ripening caused changes in those factors, which were noted. Spraying amino acids had no appreciable impact on the physical features of fruit during storage, such as weight loss, fruit colour, hardness, length, and lenticel burn. Fruit fly punctures were nonsignificant among the different chemicals as compared to the control. The maximum number was in control, i.e., 3.06±0.48 and the minimum was in Promise®, followed by Flagon® and Izabion®, i.e., 0.45±0.21, 0.72±0.15 and 0.44±0.02, respectively. Whereas biochemical parameters, total soluble solids, pH, titratable acidity, vitamin C, rag weight, juice and stone weight were also non-significantly affected by amino acid spraying. The amino acids are essential for increasing the fruit retention and output of the fruit tree. Because amino acids are essential for the majority of biological processes, enhanced quantity and export quality should be utilized.