Luteolin content and antioxidant activity in micropropagated plants of Poliomintha glabrescens (Gray)

García-Pérez, Enrique; Gutiérrez-Uribe, Janet A.; García‐Lara, Silverio

doi:10.1007/s11240-011-0055-z

Cited by 43 publications

(22 citation statements)

References 37 publications

(49 reference statements)

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“…Similar findings were observed in P. glabrescens, C. pumilum,M. officinalis, and S. corymbosa where higher antioxidant activity correlated with higher total phenolic contents and a higher free radical scavenging activity was recorded (García-pérez et al 2012;Al Khateeb et al 2012;Barros et al 2013;Mahendran & Narmatha Bai 2014).…”

Section: Discussionsupporting

confidence: 84%

An efficient in vitro propagation, antioxidant and antimicrobial activities of Aphyllorchis montana Rchb.f.

Mahendran

2015

Plant Biosystems - An International Journal Dealing with all As

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An in vitro plant regeneration protocol was successfully established in Aphyllorchis montana, a saprophytic achlorophyllous orchid by culturing immature seeds. Among the six basal media evaluated for seed germination, BM-1-Terrestrial Orchid medium was found to be the best followed by Knudson C medium. Half-strength BM-1-Terrestrial Orchid medium was supplemented with different growth regulators either individually or in combinations for multiplication of shoots induction. Of the five cytokinins tested, thidiazuron at 6.8 mM was found to be most effective for multiple shoot induction yielding 17.24^0.27 shoots after 10 weeks of culture. Addition of low concentration of a-Naphthaleneacetic acid (1.3 mM) in MS medium supplemented with the cytokinin thidiazuron (6.8 mM) favored shoot multiplication. The addition of organic additives to the medium containing thidiazuron enhanced the number of regenerated shoots. The plantlets were acclimatized, and the survival rate was 100%. Screening of the antioxidant and antimicrobial activities and estimation of total phenolics and flavonoid content of methanolic extracts of micropropagated plants were also carried out and compared with that of the wild-grown plants. In all the tests, methanolic extract from wild-grown plants showed higher antioxidant, antimicrobial activity, total phenolics and flavonoid content than in vitro propagated plants.

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Section: Discussionsupporting

confidence: 84%

An efficient in vitro propagation, antioxidant and antimicrobial activities of Aphyllorchis montana Rchb.f.

Mahendran

2015

Plant Biosystems - An International Journal Dealing with all As

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“…In general, tissue cultured and cultivated plants are not as therapeutically potent as naturally-grown plants, however this is not always the case (García-Pérez et al 2012;Baskaran et al 2013). In the current study, antibacterial activity was evaluated in in vitro, ex vitro and garden-grown mother plants tissues, however, all the in vitro and ex vitro plant tissues exhibited bioactivity against K. pneumonia.…”

Section: Discussionmentioning

confidence: 99%

In vitro propagation and antibacterial activity in Cotyledon orbiculata: a valuable medicinal plant

Kumari

Baskaran

Staden

2015

Plant Cell Tiss Organ Cult

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An effective in vitro propagation system via organogenesis was developed for Cotyledon orbiculata, a valuable medicinal plant. Plant regeneration and morphogenesis in vitro of C. orbiculata was initiated from leaf explants using various concentrations and combinations of plant growth regulators (PGRs). The highest number of regenerated shoots (20.0 ± 1.64) and shoot length (2.36 ± 0.27 cm) per explant was obtained on Murashige and Skoog (MS) medium supplemented with a combination of 5 lM thidiazuron and 2.0 lM benzyladenine. The regenerated shoots were elongated (6.47 ± 1.02 cm) when transferred to half-strength MS medium. Elongated shoots rooted (39.60 ± 1.27 per shoot) best in a half-strength MS medium containing 10 lM indole-3-butyric acid. Plantlets were successfully acclimatized (100 %) in a vermiculitesoil mixture (1:1 v/v) in the greenhouse. Rooting was also achieved ex vitro during acclimatization in a vermiculitesoil mixture (1:1 v/v) in the greenhouse with good plant growth. Different tissue types from in vitro culture (callus, shoots and plantlets) and leaves from ex vitro plants exhibited antibacterial activity [minimum inhibitory concentration (MIC) from 0.098 to 0.78 mg ml -1 ] against both Gram-positive and Gram-negative bacteria. In vitro and ex vitro developed plant tissues showed antibacterial activity (MIC of 0.195-0.78 mg ml -1 ) specifically against Klebsiella pneumoniae. Despite this, generally the leaf of ex vitro plants gave good activity (MIC of 0.195-0.78 mg ml -1 ) against both Gram-positive and Gram-negative bacteria. The present study indicated that tissue-culture-derived calli, shoots and plantlets, and ex vitro acclimatized leaves influenced therapeutic potential by the PGRs treatment used in the tissue culture system. The in vitro regenerated plants and its tissues of C. orbiculata using this protocol can be utilized as therapeutic agents for traditional medicine and for ex situ conservation.

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“…For phytochemical and antioxidant assays, the leaf and root of in vitro-grown R. glutinosa were harvested after four weeks of light treatments and extracted with methanol according to Gracia-Perez et al (2012) with slight modifications. Briefly, 0.5 g of plant tissue was homogenized using liquid nitrogen and extracted with 5 mL of 80% methanol with continuous shaking (Model 200FL, Koencon shaking incubator, Hanam City, Korea) for 5 h at 200 rpm.…”

Section: Methodsmentioning

confidence: 99%

“…The total flavonoid content (expressed in quercetin equivalent) was determined by aluminum chloride method outlined by Gracia-Perez et al (2012). Briefly, samples (0.1 mL) were made up to 1 mL with 80% methanol and used for the analysis by adding 1 mL of 2% aluminum chloride solution.…”

Section: Methodsmentioning

confidence: 99%

Blue LED light enhances growth, phytochemical contents, and antioxidant enzyme activities of Rehmannia glutinosa cultured in vitro

Manivannan

Soundararajan

Halimah

et al. 2015

Hortic. Environ. Biotechnol.

201

105

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The objective of the current study is to determine the effect of light quality on enhancement of growth, phytochemicals, antioxidant potential, and antioxidant enzyme activities at in vitro cultures of Rehmannia glutinosa Libosch. In vitro-grown shoot tip explants were cultured on the plant growth regulator (PGR)-free Murashige and Skoog (MS) medium and cultured under a conventional cool white fluorescent light (control), blue light emitting diode (LED) light or red LED light. After four weeks, the growth traits along with total phenol content, total flavonoid content, free radical scavenging activities, and antioxidant enzyme activities were measured. Interestingly, the blue or red LED treatments showed a significant increase in growth parameters compared with the cool white florescent light. In addition, the LED treatments increased the total phenol and flavonoid levels in leaf and root extracts. Furthermore, data on the total antioxidant capacity, reducing power potential, and DPPH radical scavenging capacity also revealed the enhancement of antioxidant capacity under both blue and red LED treatments. Especially, the blue LED treatment significantly increased the antioxidant enzyme activities in both the leaf and root, followed by the red LED treatment. Modulation in the spectral quality particularly by the blue LED induced the antioxidant defense line and was directly correlated with the enhancement of phytochemicals. Therefore, the incorporation of blue or red LED light sources during in vitro propagation of R. glutinosa can be a beneficial way to increase the medicinal values of the plant.Additional key words: antioxidant potential, light emitting diodes, metabolite enhancement, secondary metabolites Hort.

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Luteolin content and antioxidant activity in micropropagated plants of Poliomintha glabrescens (Gray)

Cited by 43 publications

References 37 publications

An efficient in vitro propagation, antioxidant and antimicrobial activities of Aphyllorchis montana Rchb.f.

An efficient in vitro propagation, antioxidant and antimicrobial activities of Aphyllorchis montana Rchb.f.

In vitro propagation and antibacterial activity in Cotyledon orbiculata: a valuable medicinal plant

Blue LED light enhances growth, phytochemical contents, and antioxidant enzyme activities of Rehmannia glutinosa cultured in vitro

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