In vitro propagation and antibacterial activity in Cotyledon orbiculata: a valuable medicinal plant

Abstract

An effective in vitro propagation system via organogenesis was developed for Cotyledon orbiculata, a valuable medicinal plant. Plant regeneration and morphogenesis in vitro of C. orbiculata was initiated from leaf explants using various concentrations and combinations of plant growth regulators (PGRs). The highest number of regenerated shoots (20.0 ± 1.64) and shoot length (2.36 ± 0.27 cm) per explant was obtained on Murashige and Skoog (MS) medium supplemented with a combination of 5 lM thidiazuron and 2.0 lM benzyladenine. The regenerated shoots were elongated (6.47 ± 1.02 cm) when transferred to half-strength MS medium. Elongated shoots rooted (39.60 ± 1.27 per shoot) best in a half-strength MS medium containing 10 lM indole-3-butyric acid. Plantlets were successfully acclimatized (100 %) in a vermiculitesoil mixture (1:1 v/v) in the greenhouse. Rooting was also achieved ex vitro during acclimatization in a vermiculitesoil mixture (1:1 v/v) in the greenhouse with good plant growth. Different tissue types from in vitro culture (callus, shoots and plantlets) and leaves from ex vitro plants exhibited antibacterial activity [minimum inhibitory concentration (MIC) from 0.098 to 0.78 mg ml -1 ] against both Gram-positive and Gram-negative bacteria. In vitro and ex vitro developed plant tissues showed antibacterial activity (MIC of 0.195-0.78 mg ml -1 ) specifically against Klebsiella pneumoniae. Despite this, generally the leaf of ex vitro plants gave good activity (MIC of 0.195-0.78 mg ml -1 ) against both Gram-positive and Gram-negative bacteria. The present study indicated that tissue-culture-derived calli, shoots and plantlets, and ex vitro acclimatized leaves influenced therapeutic potential by the PGRs treatment used in the tissue culture system. The in vitro regenerated plants and its tissues of C. orbiculata using this protocol can be utilized as therapeutic agents for traditional medicine and for ex situ conservation.

In Vitro Propagation via Organogenesis and Formation of Globular Bodies of Salvia Plebeia: A Valuable Medicinal Plant

“…In this study, the shoots were rooted in the same treatments. Similar ndings have been reported for other plant species (Kumari et al 2016;Kitamura et al 2002; Kordi et al 2013). A Direct adventitious bud induction from shoot tips on MS medium containing 0.1 mg/L NAA (or IAA) and…”

Improved micropropagation of Bacopa monnieri (L.) Wettst. (Plantaginaceae) and antimicrobial activity of in vitro and ex vitro raised plants against multidrug-resistant clinical isolates of urinary tract infecting (UTI) and respiratory tract infecting (RTI) bacteria

“…With a similar approach, a comparative study was conducted by few researchers in recent past [41][42][43][44]. Some of them reported the in vitro plants exhibited more antimicrobial activity as compared to ex vitro plants [42,43], whereas the majority of them reported ex vitro plants are superior or more or less comparable as compare to in vitro plants [41,44]. The in vitro plants, producing secondary metabolites at a primary stage of growth, provides an opportunity for fast production of pharmacologically important compounds that can be utilized for medicinal purposes [41].…”

Establishment of a Rapid and Efficient Micropropagation System for Succulent Plant Haworthia turgida Haw.

“…Adventitious shoot proliferation in many succulent plants relies on the crosstalk between auxins and cytokinins (Amoo et al, 2009;Kitamura et al, 2002;Solís et al, 2013;Zhao et al, 2013). The leaf explants of Cotyledon orbiculata form adventitious shoots from calli on the medium supplemented with combinations of PGRs, among which BA with NAA, and TDZ with NAA were the optimal combinations (Kumari et al, 2016). In the present study, multiple adventitious shoots were induced from calli on MS medium using various concentrations of BA and 2,4-D (Table 3).…”

“…Consequently, micropropagation has been considered as an attractive way of propagating Haworthia genus plants. This method facilitates the propagation of plants without seasonal and environment limitations and in a large scale, which are important factors in the breeding plants for ornamental and landscaping purposes (Kitamura et al, 2002;Kumari et al, 2016;Preil et al, 1988). Plantlets have been successfully induced in several Haworthia species using explants from inflorescences (Kaul and Sabharwal, 1972;Majumdar, 1970a;Ogihara, 1979;Ogihara and Tsunewaki, 1978), ovary walls (Majumdar, 1970b), and leaves (Beyl and Sharma, 1983;Rogers, 1993b).…”

“…Growth characteristics in response to various auxins and cytokinins, such as NAA, 2,4-D, and BA (Kaul and Sabharwal, 1972;Ogihara, 1979;Ogihara and Tsunewaki, 1978), have been examined in some Haworthia species. However, none of these studies have used TDZ in in vitro propagation, although it is an efficient PGR and is widely used for rapid propagation of plant tissues (Fatima and Anis, 2011;Kitamura et al, 2002;Kumari et al, 2016;Murthy et al, 1998). To the best of our propagation knowledge, there has also been no published study on in vitro propagation of H. turgida through the leaf explants either.…”