<p>Caspase Recruitment Domain-Containing Protein 9 Expression is a Novel Prognostic Factor for Lung Adenocarcinoma</p>

Abstract

Purpose Caspase recruitment domain-containing protein 9 (CARD9) is expressed at high levels in bone marrow cells and has a crucial role in innate immunity. Current studies indicate that CARD9 also plays a key role in tumor progression, but there are few reports on the role of CARD9 in lung cancer. The aim of this study was to clarify the role of CARD9 in lung adenocarcinoma. Patients and Methods Lung adenocarcinoma tumor samples from 74 patients who underwent complete resection at Kobe University Hospital from January 2014 to December 2014 were analyzed by immunohistochemistry. The role of CARD9 in cancer cells was analyzed using lung cancer cell lines treated with CARD9 siRNA. Results High expression of CARD9 was observed in 32.4% of tumors, and compared to low expression of CARD9, high expression was associated with poorer overall survival (P = 0.0365). Univariate and multivariate analyses showed that high expression of CARD9 was an independent prognostic factor. Knockdown of CARD9 in lung adenocarcinoma cells inhibited proliferation but did not increase apoptosis. In addition, CARD9 activated the NF-κB pathway in a lung adenocarcinoma cell line. Conclusion CARD9 was shown to be an independent prognostic factor of poor outcome for lung cancer and may represent a molecular target for treatment.

CARD9 as a potential therapeutic target in lung cancer

“…Pan and co-workers also identified CARD9 as a potential prognostic indicator in lung cancer [30]. Contrary to previous findings [1,13], CARD9 expression was reduced in lung cancer and exhibited anti-tumor activity in this study. A total of 94 cases diagnosed with non-small cell lung cancer were studied in this report and CARD9 expression, at both protein and mRNA levels, indicated a clear decrease in cancer tissue in comparison with adjacent normal tissues.…”

“…In this study, the investigators also showed that CARD9, in lung cancer cell lines, had a critical role in facilitating the progression of lung cancer [13]. CARD9 was upregulated in various lung cancer cell lines including PC9, A549 and II18.…”

“…Miwa and colleagues reported that CARD9 served as a prognostic marker of poor outcome in lung cancer patients [13]. In this investigation, a total of 74 lung cancer patients diagnosed with adenocarcinoma were enrolled.…”

“…Interestingly, CARD9 is highly expressed in MDSCs and exhibits a central role in regulating innate immunity [1]. Recently, it was reported that CARD9 function in MDSCs might drive a novel mechanism of lung cancer development [1,12,13]. This review focuses on our current understanding of CARD9 function in lung cancer and discusses CARD9 clinical significance, its molecular mechanism of action, as well as its potential as a therapeutic target.…”

Mycobiota and C-Type Lectin Receptors in Cancers: Know thy Neighbors

“…The role of CARD9 in lung cancer is also controversial. Miwa et al (2020) found that CARD9 was expressed in the cytoplasm of tumor cells. The high expression was associated with poorer OS compared to the low expression of CARD9 ( Miwa et al, 2020 ).…”