Low power microwave interaction with phospholipase C and D signal transduction pathways in myogenic cells

Abstract

Ionic channel proteins are possible sites of microwave interaction at the cell membrane level. Patch-clamp data, using single channel and total current recording, indicated that low level microwave fields may modify some functional parameters of the nicotinic acetylcholine receptor in primary chick myotubes, suggesting a possible effect of microwaves on myogenic cells. Here, we investigated the biological relevance of such results, in relation to the possible involvement of intracellular signaling processes. We exposed L6-C5 myogenic cells to low power electromagnetic fields and observed the consequences on hormonal activation of phospholipases C and D. We found that increased inositol phospholipid turnover, induced by acetylcholine and arginine vasopressin activation of phospholipase C, was not modified in microwave irradiated myoblasts or myotubes. Moreover, vasopressin-dependent phospholipase D activation, assessed by measuring the [3H]-free choline release, was not modified by microwave irradiation. Our conclusions suggest that low level microwave fields do not modify signal transduction pathways activated by acetylcholine and vasopressin in L6-C5 myogenic cells.

Derangement of chick embryo retinal differentiation caused by radiofrequency electromagnetic fields

“…Nonthermal effects of EMF have been reported in various studies. These include cell kinetics and proliferation effects (Velizarov et al 1999), effects on genes (Verschaeve et al 2006), signal transduction effects and alterations in membrane structure and function (Donato et al 2004), metabolic effects (Spadaro & Bergstrom 2002), effects associated with free-radical production (Lantow et al 2006), and immunological and carcinogenic effects (Bonhomme-Faivre et al 2003). Low-level radiofrequency exposure causes DNA damage (Aitken et al 2005), influences central nervous system function (Maier 2001), melatonin synthesis (Sukhotina et al 2006), and permeability of the blood brain barrier (Franke et al 2005).…”

“…1999), effects on genes (Verschaeve et al. 2006), signal transduction effects and alterations in membrane structure and function (Donato et al. 2004), metabolic effects (Spadaro & Bergstrom 2002), effects associated with free‐radical production (Lantow et al.…”

Effects of Electromagnetic Field on the Development of Chick Embryo: An In Vivo Study

“…Radio waves also induce NADH oxidase enzyme, which might play a key role in the various cellular adverse effects [36,40]. Various cellular and physiological processes can be affected as a consequence of increased levels of free radicals, including gene expression, cell growth, apoptosis, and release of calcium from intracellular storage sites [32,36,[41][42][43][44][45][46][47][48][49].…”