Long-term response on growth, antioxidant enzymes, and secondary metabolites in salicylic acid pre-treated Uncaria tomentosa microplants

Sánchez-Rojo, Silvia; Cerda-Garcı́a-Rojas, Carlos M.; Esparza-Garcı́a, F.; Plasencia, Javier; Poggi‐Varaldo, Héctor M.; Ponce-Noyola, Teresa; Ramos-Valdivia, Ana C.

doi:10.1007/s10529-015-1931-0

Cited by 15 publications

(8 citation statements)

References 34 publications

(36 reference statements)

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“…Salicylic acid, a signaling molecule, has direct or indirect effects on many physiological processes in plants by its interaction with some functional or other signaling molecules (Khan, Prithiviraj & Smith, 2003; Sánchez-Rojo et al, 2015). Regarding P max , immersion of the branch in SA solution may also reproduce in situ measurements.…”

Section: Discussionmentioning

confidence: 99%

Another choice for measuring tree photosynthesisin vitro

Chang-jun

Liu

Chai

et al. 2019

PeerJ

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BackgroundIn the case of tall trees in the field or in rugged terrain where an instrument cannot be placed operationally, beveling is a popular method used to measure in vitro photosynthesis. However, some studies and our own research have shown that net photosynthesis values measured in vitro are generally significantly lower than values measured in situ.MethodsTo develop a more accurate and applicable method for in vitro determination of photosynthesis, we evaluated five different methods for preparing detached tree branches to measure photosynthesis and gas exchange in vitro (beveling, cracking, splitting, girdling, and immersion in salicylic acid solution). Ten common tree-species were used.ResultsBy comparing light response curves and water-status data, we found that (1) it is possible, to some extent, to substitute in vitro measurement of photosynthetic characteristics of tree species for in situ measurement, provided a suitable treatment is employed; (2) the beveling method is likely to underestimate photosynthetic potential of some trees; (3) after cracking application, most detached branches effectively continued to absorb water; and (4) measurements obtained using detached tree-branches processed by the cracking method were closer to those obtained in situ in intact trees; (5) some tree species (Diospyros kaki, Eriobotrya japonica) appeared to be particularly sensitive to the cracking method, and their in-vitro maximum net photosynthesis rate (Pmax) was significantly less than the in-situ value (P < 0.05).DiscussionOur findings provide a methodological support for comprehensive and accurate measurement of plant functional traits. The use of the cracking method contributes to feasibility and reliability of the measurement of photosynthetic parameters in tall trees, thus providing more accurate photosynthetic parameters for the analysis of trade-off strategies at the leaf level.

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Section: Discussionmentioning

confidence: 99%

Another choice for measuring tree photosynthesisin vitro

Chang-jun

Liu

Chai

et al. 2019

PeerJ

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“…roseus alkaloid production by MJ combined with putrescine Protein content and assays of antioxidant enzyme activity Bradford's method was used to consider the protein content. Catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) activities were determined by standard methods as previously described in Sanchez-Rojo et al (2015).…”

Section: Alkaloid Extraction and Analysismentioning

confidence: 99%

Alkaloids production and antioxidant properties in Catharanthus roseus (L.) G. Don. shoots and study of alkaloid biosynthesis-related gene expression levels in response to methyl jasmonate and putrescine treatments as eco-friendly elicitors

2019

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“…It is generally recognized that the production and distribution of the active compounds in medicinal plants is regulated by genetic factors, harvesting time, development environment, physiological factors, and chemical factors. It has been found that exogenous plant hormone, like methyl jasmonate, abscisic acid, and salicylic acid, could affect the accumulation of secondary metabolites in Salvia miltiorrhiza [30], Glycyrrhiza uralensis root [31], and Uncaria tomentosa [32]. Exogenous plant hormone applied in the S. grosvenorii might be an appropriate approach for good production, and Zhang et al demonstrated that methyl jasmonate applied in different development stages of S. grosvenorii fruit significantly promotes the accumulation of squalene, cucurbitadienol, and mogrosides IIE [33].…”

Section: Discussionmentioning

confidence: 99%

Effects of Forchlorfenuron on the Morphology, Metabolite Accumulation, and Transcriptional Responses of Siraitia grosvenorii Fruit

et al. 2019

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Siraitia grosvenorii fruit, called luo-han-guo (LHG), have been used as a traditional Chinese medicine (TCM) and dietary supplements for many years. Mogrosides, the main bioactive ingredients in LHG, are commercially available worldwide as a non-sugar-based and noncaloric sweetener. However, the production cannot meet the increasing market demand because of the low content of mogrosides and the small size of LHG. Therefore, some advanced technologies have been applied for improving the quality of LHG. Forchlorfenuron (CPPU), a plant growth regulator, is widely applied to promote plant yield and the secondary metabolite synthesis. Here, the content of nine mogrosides and three intermediates in LHG that were treated with three different concentrations of CPPU were determined by LC-MS/MS and GC-MS, respectively. The total content of mogrosides in LHG treated with CPPU was not enhanced, and the proportion of some main bioactive ingredients, including mogroside V (MV), were decreased relative to that of the control treatment. Morphological and cytological observations showed CPPU could make an early lignification in fruit epidermal cells, and 5 or 25 mg L−1 CPPU could inhibit LHG growth. The expression levels of 24 key genes in the mogroside biosynthesis pathway were measured and revealed that genes downregulated in upstream, and different expressions of SgUGTs would affect the accumulations and proportions of mogrosides in LHG induced by CPPU. This was the first study that applied CPPU individually on LHG, and assessed effects of CPPU on the morphology, the accumulation of metabolites, and expression profiles of 24 structural genes. The CPPU effects on LHG were undesirable, including development inhibition and the decrease of main mogroside content. These will provide guidance for the rational application of CPPU.

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Long-term response on growth, antioxidant enzymes, and secondary metabolites in salicylic acid pre-treated Uncaria tomentosa microplants

Abstract: Pre-treatment with 1 µM SA of U.tomentosa microplants preserved the survival rate and increased oxindole alkaloids, flavonoids, and phenolic compounds in correlation with H2O2 and peroxidase activity enhancements, offering biotechnological advantages over non-treated microplants.

Cited by 15 publications

References 34 publications

Another choice for measuring tree photosynthesisin vitro

Another choice for measuring tree photosynthesisin vitro

Alkaloids production and antioxidant properties in Catharanthus roseus (L.) G. Don. shoots and study of alkaloid biosynthesis-related gene expression levels in response to methyl jasmonate and putrescine treatments as eco-friendly elicitors

Effects of Forchlorfenuron on the Morphology, Metabolite Accumulation, and Transcriptional Responses of Siraitia grosvenorii Fruit

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