Alkaloids production and antioxidant properties in <i>Catharanthus roseus</i> (L.) G. Don. shoots and study of alkaloid biosynthesis-related gene expression levels in response to methyl jasmonate and putrescine treatments as eco-friendly elicitors

Khataee, Elham; Karimi, Farah; Razavi, Khadijeh

doi:10.1556/019.70.2019.05

Cited by 16 publications

(8 citation statements)

References 29 publications

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“…Consistent with these findings, an increase has been reported in the accumulation of vindoline in the transgenic Datexpressing plants [12]. Furthermore, MeJA and putrescine treatments have been shown to lead to the up-regulation of Str, Prx1, Dat genes and consequently the production of vincristine and vinblastine alkaloids [35]. In another study, foliar application of SA on the leaves of C. roseus seedlings exposed to drought and salinity stresses increased the transcript level of the Dat and D4h genes [11].…”

Section: Discussionsupporting

confidence: 62%

“…DAT converts deacetylvindoline into vindoline in the TIAs alkaloid pathway [15,6,8]. An increase in the expression level of Dat has been found to result in the accumulation of vinblastine and vincristine alkaloids [12,35]. Inoculation of C. roseus with fungal endophytes led to an increase in the expression of Str, D4h and Dat genes and consequently vindoline content C. roseus increased [30].…”

Section: Discussionmentioning

confidence: 99%

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The expression of Terpenoid Indole Alkaloid (TIAs) pathway genes in Catharanthus roseus in response to salicylic acid treatment

et al. 2020

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Vinblastine and vincristine are two important anti-cancer drugs that are synthesized by the Terpenoid Indole Alkaloids (TIAs) pathway in periwinkle (Catharanthus roseus). The major challenge in the pharmaceutical industry is the low production rate of these alkaloids. TIAs pathway is flexible and is affected by elicitors, such as Salicylic Acid (SA).This study aimed to investigate the expression pattern of some key genes in TIAs pathway under SA treatment. Foliar application of SA (0.01 and 0.1 mM) was used and leaves samples were taken at 0, 12, 18, 24 and 48 hours after the treatment. qRT-PCR was used to investigate the expression pattern of Chorismate mutase (Cm), Tryptophan decarboxylase (Tdc), Geraniol-10-hydroxylase (G10h), Secologanin synthase (Sls), Strictosidine synthase (Str), Desacetoxyvindoline-4-hydroxylase (D4h) and Deacetylvindoline-4-O-acetyltransferase (Dat) genes, following the SA treatment. The results of this experiment showed that transcript levels of Tdc, G10h, Sls, Str, D4h and Dat genes were significantly up-regulated in both SA concentration treatments. Furthermore, the highest transcript levels of Dat was observed after 48 hours of the SA treatments. qRT-PCR results suggests that SA induces transcription of major genes involved in alkaloids biosynthesis in Catharanthus roseus. It can be concluded that up-regulation of Tdc, G10h, Sls, Str, D4h and Dat genes can result in a higher production rate of vinblastine and vincristine alkaloids.

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Section: Discussionsupporting

confidence: 62%

Section: Discussionmentioning

confidence: 99%

The expression of Terpenoid Indole Alkaloid (TIAs) pathway genes in Catharanthus roseus in response to salicylic acid treatment

et al. 2020

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“…Considering the positive correlation between relative expression level of these gene and the accumulation of corresponding alkaloids in C. roseus (Dutta et al, 2005;Goklani et al, 2009;Jaggi et al, 2011 andWang et al 2016), it can be concluded that up-regulation of G10H, DAT, T16H and CrPRX genes under these treatments, especially by P. uorescens, can result in a higher production rate of vinblastine and vincristine alkaloids as nal products in the TIA biosynthetic pathway. As an example, it is shown that an increase in the expression level of DAT has been found to result in the accumulation of vinblastine and vincristine alkaloids (Wang et al, 2012 andKhataee et al, 2019). In this case, metabolite studies were performed in the plant.…”

Section: Discussionmentioning

confidence: 99%

Effect of the Plant Probiotic Bacteria on TIA Biosynthesis Pathway Gene Expression Profiling, Vinblastine and Vincristine Content in the Root of Catharanthus Roseus

Ahmadzadeh

Keshtkar

Moslemkhany

et al. 2021

Preprint

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Catharanthus roseus is the sole resource of vinblastine and vincristine, which are two of the biggest concerns of TIAs because of their powerful anticancer activities. Increasing the concentration of these alkaloids in various organs of the plant is one of the important goals in C. roseus breeding programs. Plant probiotic bacteria (PBB) act as biotic elicitors and can induce the synthesis of secondary products in plants. The purpose of this research is to study the individual and combined effects of P. fluorescens and A. brasilense on expression of the TIA biosynthetic pathway genes (G10H, DAT, T16H and CrPRX) using qRT-PCR and the content of vinblastine and vincristine alkaloids using HPLC method in roots of C. roseus. P. fluorescens drastically increased the content of vinblastine and vincristine alkaloids, compared to the control in the roots, up to 174 and 589 (µg/g), respectively. According to the molecular analysis, bacterium significantly increased the expression of more genes in the TIA biosynthetic pathway compared to the control. P. fluorescens increased the expression of the final gene of the biosynthetic pathway (CrPRX) 47.9 times compared to the control. Therefore, the findings indicate the coordination of transcriptional and metabolic outcomes. The same result was also observed for A. brasilense. According to the results, it can be concluded that, the seed priming and root of the seedling treatments of probiotic bacteria can be used as a good tool in the enhancement of alkaloid contents in medicinal plants, as it provides an eco-friendly approach.

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“…MAPK has been known to play an important function in plant responses, triggered by ROS, through dynamic gene expression and regulations. It has been reported to be crucial for flavonoid [80] and alkaloid [81] biosynthesis when plants are subjected to stress conditions. Paezi et al [82] reported up-regulation of several genes involved in the biosynthesis of alkaloids, coupled with high expression of Mitogen-Activated Protein Kinase 3 (CrMPK3) gene on Catharanthus roseus after silver exposure.…”

Section: Possible Mechanism Of Agnps In Plant Cellsmentioning

confidence: 99%

Nanotechnology in Plant Metabolite Improvement and in Animal Welfare

et al. 2022

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Plant tissue culture plays an important role in plant biotechnology due to its potential for massive production of improved crop varieties and high yield of important secondary metabolites. Several efforts have been made to ameliorate the effectiveness and production of plant tissue culture, using biotic and abiotic factors. Nowadays, the addition of nanoparticles as elicitors has, for instance, gained worldwide interest because of its success in microbial decontamination and enhancement of secondary metabolites. Nanoparticles are entities in the nanometric dimension range: they possess unique physicochemical properties. Among all nanoparticles, silver-nanoparticles (AgNPs) are well-known for their antimicrobial and hormetic effects, which in appropriate doses, led to the improvement of plant biomass as well as secondary metabolite accumulation. This review is focused on the evaluation of the integration of nanotechnology with plant tissue culture. The highlight is especially conveyed on secondary metabolite enhancement, effects on plant growth and biomass accumulation as well as their possible mechanism of action. In addition, some perspectives of the use of nanomaterials as potential therapeutic agents are also discussed. Thus, the information provided will be a good tool for future research in plant improvement and the large-scale production of important secondary metabolites. Elicitation of silver-nanoparticles, as well as nanomaterials, function as therapeutic agents for animal well-being is expected to play a major role in the process. However, nanosized supramolecular aggregates have received an increased resonance also in other fields of application such as animal welfare. Therefore, the concluding section of this contribution is dedicated to the description and possible potential and usage of different nanoparticles that have been the object of work and expertise also in our laboratories.

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Alkaloids production and antioxidant properties in Catharanthus roseus (L.) G. Don. shoots and study of alkaloid biosynthesis-related gene expression levels in response to methyl jasmonate and putrescine treatments as eco-friendly elicitors

Abstract: Alkaloids production and antioxidant properties in Catharanthus roseus (L.) G. Don. shoots and study of alkaloid biosynthesisrelated gene expression levels in response to methyl jasmonate and putrescine treatments as ecofriendly elicitors. Biol. Fut. 70,[38][39][40][41][42][43][44][45][46]

Cited by 16 publications

References 29 publications

The expression of Terpenoid Indole Alkaloid (TIAs) pathway genes in Catharanthus roseus in response to salicylic acid treatment

The expression of Terpenoid Indole Alkaloid (TIAs) pathway genes in Catharanthus roseus in response to salicylic acid treatment

Effect of the Plant Probiotic Bacteria on TIA Biosynthesis Pathway Gene Expression Profiling, Vinblastine and Vincristine Content in the Root of Catharanthus Roseus

Nanotechnology in Plant Metabolite Improvement and in Animal Welfare

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