sensing principle of FETs is the adsorption of charged analytes on the sensor surface, which results in a change of electrical potential to induce a detectable signal in the form of current change. A new approach for enhanced sensitivity is suggested based on the single trap phenomena [2] and demonstrated in nanowire devices with a liquid gate filled with different biochemical liquids including phosphate buffered saline (PBS). The actual biochemical detection is often in a solution system where the solid-liquid interface responds to the complex multiion environment. To expand the sensor applications and verify signal reliability, it is essential to study the effect of ion concentrations in biochemical solutions on the sensor-solution interface, especially for small analyte concentrations.To study the composition of the ion effect at the sensor-solution interface and the electrical signal response of the device to different ions is of great significance for the expansion of the sensor detection principles and the verification of signal registration reliability, especially for small concentrations of biochemical solutions.Nanostructures have already been confirmed to be an ultrasensitive platform to study the monovalent ion system (e.g. KCl and NaCl) and the divalent ion system in nanofluidic ionic transport. [3] Charge inversion phenomena can be observed during the sensing measurement, which means that more counterions can be found inside the electric double layer than are needed to compensate for the surface charge. Though this phenomenon was reported a century ago, recent research suggests that the ion-ion correlation is the likely origin of charge inversion. [4] The site-binding mode (SBM) mechanism [5] of inorganic salt ions on the oxide surface was previously reported. [6] The semiempirical Nikolsky theory is also used to estimate the selectivity of sensors against interfering ions. [7] The FET sensing ability typically requires the assistance of a given surface coating to promote the selectivity of ion sensing. [8] Sivakumarasamy [9] et al. developed a silicon nanotransistor with sizes scaled down to ≈25 nm to exploit ion-specific surface interaction without requiring a selective organic layer. They tested this method with blood serum containing Na + , K + , Ca 2+ , and Mg 2+ . A lack of understanding of ion-surface interaction on pure solid semiconductor materials gives rise to many inconsistencies in the same sensors for different biochemical sensing systems.High-quality, liquid-gated two-layer (TL) nanowire (NW) field-effect transistor (FET) structures are fabricated and studied in different operation regimes and with different concentrations of MgCl 2 solutions. The space-charge-limited current (SCLC) effect is sensitive to surface phenomena and is registered at the drain-source voltage (V DS ) above 0.14 V, and analyzed using several methods including noise spectroscopy. Noise spectra reflect the clear transformation from 1/f to 1/ (f in power 1.5) behavior, confirming the SCLC effect, which is sensit...